A5071278263- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Hydrogels: synthesis, properties, applications
- Additive Manufacturing and 3D Printing Technologies
- Advanced biosensing and bioanalysis techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cardiac Valve Diseases and Treatments
- Cellular Mechanics and Interactions
- Biosensors and Analytical Detection
- Advanced Materials and Mechanics
- Advanced Sensor and Energy Harvesting Materials
- Neuroscience and Neural Engineering
- Microfluidic and Bio-sensing Technologies
- Micro and Nano Robotics
- Multiple Myeloma Research and Treatments
- Mesenchymal stem cell research
- Bone and Dental Protein Studies
- Nanoplatforms for cancer theranostics
- Ubiquitin and proteasome pathways
- Chemical and Physical Properties in Aqueous Solutions
- Wound Healing and Treatments
- Histone Deacetylase Inhibitors Research
- Nanofabrication and Lithography Techniques
University of Massachusetts Lowell
2017-2025
University of Massachusetts Chan Medical School
2020-2025
Brigham and Women's Hospital
2010-2024
Harvard–MIT Division of Health Sciences and Technology
2010-2024
Massachusetts Institute of Technology
2010-2024
Harvard University
2010-2024
Innovation Research Center
2014-2017
Harvard University Press
2012-2013
Iowa State University
2008-2012
Biomimetic hybrid hydrogels have generated broad interest in tissue engineering and regenerative medicine. Hyaluronic acid (HA) gelatin (hydrolyzed collagen) are naturally derived polymers biodegradable under physiological conditions. Moreover, collagen HA major components of the extracellular matrix (ECM) most tissues (e.g., cardiovascular, cartilage, neural). When used as a material, HA-gelatin may enable mimicking ECM native tissues. Although promising biomimetic substrates, their...
To engineer tissue-like structures, cells must organize themselves into three-dimensional (3D) networks that mimic the native tissue microarchitecture. Microfabricated hydrogel substrates provide a potentially useful platform for directing biomimetic architecture in vitro. Here, we present microgrooved methacrylated gelatin hydrogels as suitable to build muscle-like fibrous structures facile and highly reproducible fashion. Microgrooved with two different ridge sizes (50 100 μm) were...
Establishing the 3D microscale organization of cells has numerous practical applications, such as in determining cell fate (e.g., proliferation, migration, differentiation, and apoptosis) making functional tissue constructs. One approach to spatially pattern is by dielectrophoresis (DEP). DEP characteristics that are important for manipulation, high accuracy, speed, scalability, ability handle both adherent non-adherent cells. However, widespread application this method largely restricted...
Cardiac failure is a critical condition that results in life-threatening consequences. Due to limited number of organ donors, tissue engineering has emerged generate functional constructs and provide an alternative mean repair regenerate damaged heart tissues. In this paper, we review the emerging directions associated with cardiac approaches. particular, discuss use hydrogels regeneration hearts. Because their tissue-like biological, chemical mechanical properties, represent potentially...
Due to the increasing demand generate thick and vascularized tissue engineered constructs, novel strategies are currently being developed. An emerging example is generation of oxygen-releasing biomaterials tackle mass transport diffusion limitations within tissue-like constructs. Biomaterials containing oxygen releasing molecules can be fabricated in various forms such as, hybrid thin films, microparticles, or three dimensional (3D) scaffolds. In this perspective, we will summarize reagents...
Microfabrication technology provides a highly versatile platform for engineering hydrogels used in biomedical applications with high-resolution control and injectability. Herein, we present strategy of microfluidics-assisted fabrication photo-cross-linkable gelatin microgels, coupled providing protective silica hydrogel layer on the microgel surface to ultimately generate gelatin-silica core–shell microgels as vitro cell culture injectable tissue constructs. A microfluidic device having...
Development of three dimensional (3D) microenvironments that direct stem cell differentiation into functional types remains a major challenge in the field regenerative medicine. Here, we describe new platform to address this by utilizing robotic microarray spotter for testing fates inside various miniaturized cell-laden gels systematic manner. To demonstrate feasibility our platform, evaluated osteogenic human mesenchymal cells (hMSCs) within combinatorial 3D niches. We were able identify...
Abstract Engineering three-dimensional (3D) tissues in clinically relevant sizes have demonstrated to be an effective solution bridge the gap between organ demand and dearth of compatible donors. A major challenge clinical translation tissue-engineered constructs is lack vasculature support adequate supply oxygen nutrients post-implantation. Previous efforts improve vascularization engineered not been commensurate meeting demands implanted during process homogeneous integration with host....
Tissue engineered heart valves (TEHV) can be useful in the repair of congenital or acquired valvular diseases due to their potential for growth and remodeling. The development biomimetic scaffolds is a major challenge valve tissue engineering. One most important structural characteristics mature leaflets intrinsic anisotropy, which derived from microstructure aligned collagen fibers extracellular matrix (ECM). In present study, directional electrospinning technique used fabricate fibrous...
Hydrogels commonly used in tissue engineering are mechanically soft, thus often display structural weakness. Herein, we introduce a strategy for enhancing the integrity and fracture toughness of cell-laden hydrogels by incorporating three-dimensional (3D) microfabricated scaffold as element. A digital micromirror device projection printing (DMD-PP) system, rapid prototyping technology which employs layer-by-layer stereolithographic approach, was utilized to efficiently fabricate 3D scaffolds...
Oxygen is essential to cell survival and tissue function. Not surprisingly, ischemia resulting from myocardial infarction induces death necrosis. Attempts regenerate with based therapies exacerbate the hypoxic stress by further increasing metabolic burden. In consequence, implanted engineered cardiac tissues suffer hypoxia-induced death. Here, we report on generation of oxygen-generating hydrogels composed calcium peroxide (CPO) laden gelatin methacryloyl (GelMA). CPO-GelMA released...
Recent innovations in bone tissue engineering have introduced biomaterials that generate oxygen to substitute vasculature. This strategy provides the immediate required for viability and graft maturation. Here we demonstrate a novel oxygen-generating scaffold with predictable release kinetics modular material properties. These hydrogel scaffolds were reinforced microparticles comprised of emulsified calcium peroxide (CaO
Engineered skeletal muscle tissues could be useful for applications in tissue engineering, drug screening, and bio-robotics. It is well-known that cells are able to differentiate under electrical stimulation (ES), with an increase myosin production, along the formation of myofibers contractile proteins. In this study, we describe use interdigitated array electrodes as a novel platform electrically stimulate engineered tissues. The resulting were analyzed quantified terms their myotube...