A5100380343- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- HER2/EGFR in Cancer Research
- Carbohydrate Chemistry and Synthesis
- Natural product bioactivities and synthesis
- Bioactive Natural Diterpenoids Research
- Protein purification and stability
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Proteoglycans and glycosaminoglycans research
- T-cell and B-cell Immunology
- Phytochemical compounds biological activities
- Ion-surface interactions and analysis
- Immune Cell Function and Interaction
- Infant Nutrition and Health
- Virus-based gene therapy research
- Chronic Lymphocytic Leukemia Research
- Nuclear Materials and Properties
- Fusion materials and technologies
- CAR-T cell therapy research
- Helicobacter pylori-related gastroenterology studies
- Breastfeeding Practices and Influences
- Viral Infectious Diseases and Gene Expression in Insects
- Genomics and Phylogenetic Studies
Sun Yat-sen University
2025
Chinese Academy of Sciences
2015-2024
Northwest A&F University
2024
Xijing Hospital
2024
Air Force Medical University
2024
National Clinical Research Center for Digestive Diseases
2024
Institute of Biophysics
2015-2023
China International Science and Technology Cooperation
2023
Children's Hospital of Chongqing Medical University
2023
Chongqing Medical University
2023
The mechanisms that promote the generation of new coronary vasculature during cardiac homeostasis and after injury remain a fundamental clinically important area study in cardiovascular field. Recently, it was reported mesenchymal-to-endothelial transition (MEndoT) contributes to substantial numbers endothelial cells myocardial infarction. Therefore, MEndoT has been proposed as paradigm mediating neovascularization is considered promising therapeutic target regeneration. Here, we show...
Significance Bunyaviruses are emerging zoonotic pathogens of public-health concern. Lack structures for proteins on the viral membrane (“envelope”) surface limits understanding entry. We describe atomic-level globular “head” envelope protein, glycoprotein N (Gn), from two members, severe fever with thrombocytopenia syndrome virus (SFTSV) and Rift Valley (RVFV), Phleboviruses genus in bunyavirus family, a structure SFTSV Gn bound neutralizing antibody Fab. The results show folded define...
Glycosylation is a topic of intense current interest in the development biopharmaceuticals because it related to drug safety and efficacy. This work describes results an interlaboratory study on glycosylation Primary Sample (PS) NISTmAb, monoclonal antibody reference material. Seventy-six laboratories from industry, university, research, government, hospital sectors Europe, North America, Asia, Australia submitted total 103 reports glycan distributions. The principal objective this was...
Abstract The ternary structure that combines fullerene and nonfullerene acceptors in a photoactive layer is demonstrated as an effective approach for boosting the power conversion efficiencies (PCEs) of organic solar cells (OSCs). Here, highly efficient OSCs comprising wide‐bandgap polymer donor (PBT1‐C), narrow‐bandgap acceptor (IT‐2F), typical derivative (PC 71 BM) are reported. It found addition PC BM into PBT1‐C:IT‐2F blend not only increases device efficiency up to 12.2%, but also...
Changes in IgG glycosylation, as a novel pathological feature, are observed various autoimmune diseases (AIDs). The glycosylation patterns of play critical role regulating the biological function and stability involved pathophysiology many AIDs. However, intracellular regulatory mechanisms underlying effects disturbances cytokines on poorly understood. Thus, we investigated elevated AIDs within B cells.First, established controlled primary culture system vitro to differentiate human CD19+...
Aberrant protein glycosylation has been shown to be associated with disease progression and can potentially useful as a biomarker if disease-specific identified. However, high-throughput quantitative analysis of derived from clinical specimens presents technical challenges due the typically high complexity biological samples. In this study, mass spectrometry-based analytical method was developed measure different glycosylated forms glycoproteins complex samples by coupling glycopeptide...
Aberrant glycosylation is a fundamental characteristic of progression diseases such as cancer. Therefore, characterization patterns proteins from disease tissues may identify changes specific to the development and improve diagnostic performance. Thus, analysis strategies with sufficient sensitivity for evaluation in clinical specimens are needed. Here, we describe an analytical strategy detection verification patterns. It based on two-phase platform including pattern discovery phase using...
Glycosylation is one of the most common protein modifications and involved in many functions glycoproteins. Investigating aberrant glycosylation associated with diseases useful improving disease diagnostics. Due to nontemplate nature glycan biosynthesis, glycans attached glycoproteins are enormously complex; thus, a method for comprehensive analysis from biological or clinical samples needed. Here, we describe novel glycomic using glycoprotein immobilization extraction (GIG). Proteins...
Carcinoembryonic antigen (CEA) is a glycoprotein associated with colorectal cancer (CRC). While the functions of its gene and protein have been fully characterized, post-translational modifications in context CRC development remain undefined.To show correlation between different stages changes glycosylation patterns CEA, we analyzed CEA tumor tissues (CEA-T) paired tumor-adjacent normal (CEA-A) from 53 patients using high-density lectin microarray containing 56 plant lectins.We detected...
Purpose: To generate and characterize a murine GITR ligand fusion protein (mGITRL-FP) designed to maximize valency the potential agonize receptor for cancer immunotherapy.Experimental Design: The EC50 value of mGITRL-FP was compared with an anti-GITR antibody in vitro agonistic cell-based reporter assay. We assessed impact dose, schedule, Fc isotype on antitumor activity T-cell modulation CT26 tumor model. OX40L-FP targeting OX40, B16F10-Luc2 models. Combination antibodies PD-L1, PD-1, or...
The constrained cross-talk between myeloid cells and T in the tumor immune microenvironment (TIME) restricts cancer immunotherapy efficacy, whereas underlying mechanism remains elusive. Parkin, an E3 ubiquitin ligase renowned for mitochondrial quality control, has emerged as a regulator of response. Here, we show that both systemic macrophage-specific ablations Parkin mice lead to attenuated progression prolonged mouse survival. By single-cell RNA-seq flow cytometry, demonstrate deficiency...
IgG glycosylation differs in rheumatoid arthritis (RA) and osteoarthritis (OA), which should contribute to their pathogenesis research diagnosis.
Twelve new diterpenoids based on two rare skeletal types, namely, paralianones A–D (1–4) and pepluanols A–H (5–12), along with five known compounds, were isolated from an acetone extract of Euphorbia peplus. Their structures proposed 1D 2D NMR spectroscopic data analysis. These evaluated for potential anti-inflammatory activity in a lipopolysaccharide-stimulated mouse macrophage cellular model. Compounds 3, 4, 11, 13, 16 displayed moderate inhibitory effects NO inhibition, IC50 values...
Abstract Phytochemical investigation of whole plants Euphorbia pilosa led to the isolation and identification two new daphnane‐diterpenoid glucosides, euphopilosides A B ( 1 2 , resp.), a ent ‐abietane, euphopilolide 3 ), together with eight known compounds. Compounds are first daphnane‐type diterpenoid glycosides. Their structures were elucidated by combination 1D‐ 2D‐NMR, MS analyses, acid hydrolysis. – 9 evaluated for their in vitro cytotoxicities against five human tumor cell lines,...
Glycosylation is one of the most common protein modifications. Each glycoprotein can be glycosylated at multiple glycosites, and each glycosites modified by different glycans. Due to this heterogeneity glycosylation, it has proven difficult study structure-function relationship specific glycans their affected glycoproteins. Here, we report a novel method for rapid quantitative identification glycoproteins containing Lectin affinity isolations are followed chemical immobilization captured...
Aberrant changes of N-glycan modifications on proteins have been linked to various diseases including different cancers, suggesting possible avenue for exploring their etiologies based N-glycomic analysis. Changes in patterns during epithelial ovarian cancer development so far investigated mainly using serum, plasma, ascites, and cell lines. However, N-glycans tumor tissues progression remained largely undefined. To investigate whether correlate with oncogenesis cancer, we profiled from...
Abstract Targeted and enantioselective delivery of chiral diagnostic-probes therapeutics into specific compartments inside cells is utmost importance in the improvement disease detection treatment. The classical DNA ‘light-switch’ ruthenium(II)-polypyridyl complex, [Ru(DIP)2(dppz)]Cl2 (DIP = 4,7-diphenyl-1,10-phenanthroline, dppz dipyridophenazine) has been shown to be accumulated only cytoplasm membrane, but excluded from its intended nuclear target. In this study, cationic...