A5080522351- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Monoclonal and Polyclonal Antibodies Research
- Proteoglycans and glycosaminoglycans research
- Galectins and Cancer Biology
- Extracellular vesicles in disease
- Parasites and Host Interactions
- Parasitic Diseases Research and Treatment
- Mass Spectrometry Techniques and Applications
- Enzyme Structure and Function
- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Immunotherapy and Immune Responses
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- MicroRNA in disease regulation
- Allergic Rhinitis and Sensitization
- Nanoplatforms for cancer theranostics
- Bacterial Identification and Susceptibility Testing
- Ion-surface interactions and analysis
- Chemical Synthesis and Analysis
- RNA Interference and Gene Delivery
- Transgenic Plants and Applications
- Animal health and immunology
- Phytochemical compounds biological activities
CIC biomaGUNE
2015-2025
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2015-2025
Digital Research Alliance of Canada
2020-2025
Boeing (Spain)
2020
Ayuntamiento de San Sebastián
2020
CIC bioGUNE
2014-2015
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2014
Max Planck Institute of Colloids and Interfaces
2014
Centro de Investigaciones Científicas Isla de la Cartuja
2003-2005
Instituto de Investigaciones Químicas
2003-2005
Extracellular vesicles (EVs) are important mediators of cell-cell communication in a broad variety physiological contexts. However, there is ambiguity around the fundamental mechanisms by which these effects transduced, particularly relation to their uptake recipient cells. Multiple modes cellular entry have been suggested and we further explored role glycans as potential determinants uptake, using EVs from murine hepatic cell lines AML12 MLP29 independent yet comparable models. Lectin...
Carbohydrates participate in almost every aspect of biology from protein sorting to modulating cell differentiation and cell–cell interactions. To date, the majority data gathered on glycan expression has been obtained via analysis with either anti-glycan antibodies or lectins. A detailed understanding specificities these reagents is critical carbohydrates biological systems. Glycan microarrays are increasingly used determine binding specificity glycan-binding proteins (GBPs). In this study,...
Glycosylation is a topic of intense current interest in the development biopharmaceuticals because it related to drug safety and efficacy. This work describes results an interlaboratory study on glycosylation Primary Sample (PS) NISTmAb, monoclonal antibody reference material. Seventy-six laboratories from industry, university, research, government, hospital sectors Europe, North America, Asia, Australia submitted total 103 reports glycan distributions. The principal objective this was...
Abstract As cellular‐derived vesicles largely maintain the biomolecule composition of their original tissue, exosomes, which are found in nearly all body fluids, have enormous potential as clinical disease markers. A major bottleneck development exosome‐based diagnostic assays is challenging purification these vesicles; this requires time‐consuming and instrument‐based procedures. We employed lectin arrays to identify lectins probes for affinity‐based isolation exosomes from urinary matrix....
Abstract An effective chemoenzymatic strategy is reported that has allowed the construction, for first time, of a focused microarray synthetic N ‐glycans. Based on modular approaches, variety ‐glycan core structures have been chemically synthesized and covalently immobilized glass surface. The printed were then enzymatically diversified by action three different glycosyltransferases in nanodroplets placed top individual spots printing robot. Conversion was followed lectin binding specific...
The synthesis of a collection 33 xylosylated and core-fucosylated N-glycans found only in nonmammalian organisms such as plants parasitic helminths has been achieved by employing highly convergent chemo-enzymatic approach. influence these core modifications on the interaction with plant lectins, human lectin DC-SIGN (Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Nonintegrin), serum antibodies from schistosome-infected individuals was studied. Core xylosylation markedly...
Two recombinant fucosyltransferases were employed as synthetic tools in the chemoenzymatic synthesis of core fucosylated N-glycan structures. Enzyme substrates rapidly identified by incubating a microarray N-glycans with transferases and detecting presence fucose four lectins one antibody. Selected then enzymatically solution on preparative scale characterized NMR MS. With this approach series α1,3-, α1,6-, difucosylated structures was accomplished very short time high yields, which...
Here, we describe a strategy for the rapid preparation of pure positional isomers complex N-glycans to complement an existing array comprising larger number and smaller glycan structures. The expanded was then employed study context-dependent binding structural fragments by monoclonal antibodies C-type lectins. A partial enzymatic elongation semiprotected core structures combined with protecting-group-aided separation preparative HPLC. This methodology, which avoids laborious chemical...
The dendritic cell-specific intracellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) is an important receptor of the immune system. Besides its role as pathogen recognition (PRR), it also interacts with endogenous glycoproteins through specific self-glycan epitopes, like LeX. However, this lectin represents a paradigmatic case glycan binding promiscuity, and has been shown to recognize antigens α1−α2 linked fucose, such histo blood group antigens, similar affinities Herein, we have...
Active arrays: Complex lipid-tagged oligosaccharides, including large multiantennary species, can be efficiently immobilized on self-assembled monolayers of alkyl mercaptans (see picture). These arrays used to follow the action a galactosyltransferase (GalT) and hydrolase. The utility system for selective trapping identification lectin from complex mixture was also demonstrated.
By combining electrophoretic protein separation with lectin-array-based glycan profiling into a single experiment, we have developed high-throughput method for the rapid analysis of glycosylation in biofluids. Fluorescently tagged proteins are separated by SDS-PAGE and transferred diffusion to microscope slide covered multiple copies 20 different lectins, where they trapped specific carbohydrate interactions while retaining their relative locations on gel. A fluorescence scan then provides...
Methods for the absolute quantification of glycans are needed in glycoproteomics, during development and production biopharmaceuticals clinical analysis glycan disease markers. Here we present a strategy chemo-enzymatic synthesis 13C labeled N-glycan libraries provide an example their use as internal standards profiling mAb by matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry. A synthetic biantennary precursor was 13C-labeled on all four amino sugar...
Due to their electrical conductivity and optical transparency, slides coated with a thin layer of indium tin oxide (ITO) are the standard substrate for protein imaging mass spectrometry on tissue samples by MALDI-TOF MS. We have now studied rf magnetron sputtering deposition parameters prepare ITO films glass substrates required nanometric surface structure use in matrix-free metabolites small-molecule drugs, without affecting transparency classical histology. The custom-made surfaces were...
Glycans play a key role as recognition elements in the communication of cells and other organisms. Thus, analysis carbohydrate-protein interactions has gained significant importance. In particular, nuclear magnetic resonance (NMR) techniques are considered powerful tools to detect relevant features interaction between sugars their natural receptors. Here, we present results obtained study on molecular different mannose-containing glycans by Pisum sativum agglutinin. NMR experiments supported...
The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). A better understanding of its immunogenicity can be important for development improved diagnostics, therapeutics, and vaccines. Here, we report longitudinal analysis three patients with moderate (#1) mild disease (#2 #3). Antibody serum responses were analyzed using spike glycoprotein enzyme linked immunosorbent assay (ELISA), full-proteome peptide, glycan microarrays. ELISA...
The specific interaction of lectins with carbohydrates and glycoconjugates grants these proteins a distinct ability to decode the glycocode. Essential for various biological processes in all organisms, this carbohydrate-binding activity also establishes as valuable tools fields such glycomics, medicine, biotechnology. Considering that discovery novel unique binding profiles is particularly relevant, study investigated specificity two extracted from Bauhinia seeds toward simple sugars,...
A novel ester type linker which upon cleavage releases the glycans as carbamate protected aminoglycosides was successfully employed in sequential assembly of L-idose and azido glucose monosaccharide building blocks to heparan sulfate precursors.
Glycan arrays have been established as the premier technical platform for assessing specificity of carbohydrate binding proteins, an important step in functional glycomics research. Access to large libraries well-characterized oligosaccharides remains a major bottleneck glycan array research, and this is particularly true glycosaminoglycans (GAGs), class linear sulfated polysaccharides which are present on most animal cells. Solid-supported synthesis potentially powerful tool accelerated...