A5101527432- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Heat shock proteins research
- Galectins and Cancer Biology
- Monoclonal and Polyclonal Antibodies Research
- Infant Nutrition and Health
- Signaling Pathways in Disease
- Toxin Mechanisms and Immunotoxins
- Proteoglycans and glycosaminoglycans research
- Escherichia coli research studies
- Estrogen and related hormone effects
- Viral gastroenteritis research and epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Respiratory viral infections research
- thermodynamics and calorimetric analyses
- Genomics and Phylogenetic Studies
- Reproductive System and Pregnancy
- Enzyme Production and Characterization
- Lysosomal Storage Disorders Research
- Immune Cell Function and Interaction
- Insect and Pesticide Research
- Virus-based gene therapy research
- Parasites and Host Interactions
Emory University
2013-2022
National Research Institute of Brewing
2021
AID Atlanta
2016
Woodruff Health Sciences Center
2016
John Wiley & Sons (United States)
2016
Stony Brook University
2014
University of Gothenburg
2011
Mayo Clinic in Arizona
1988-2008
Rega Institute for Medical Research
2008
KU Leuven
2008
A new minimal medium for enterobacteria has been developed. It supports growth of Escherichia coli and Salmonella typhimurium at rates comparable to those any the traditional media that have high phosphate concentrations, but each macronutrients (phosphate, sulfate, nitrogen) is present a sufficiently low level permit isotopic labeling. Buffering capacity provided by an organic dipolar ion, morpholinopropane sulfonate, which desirable pK (7.2) no apparent inhibitory effect on growth. The...
Human galectins have functionally divergent roles, although most of the members galectin family bind weakly to simple disaccharide lactose (Galbeta1-4Glc). To assess specificity galectin-glycan interactions in more detail, we explored binding several important (Gal-1, Gal-2, and Gal-3) using a dose-response approach toward glycan microarray containing hundreds structurally diverse glycans, compared these results determinants on cells. All three exhibited differences characteristics. On both...
The antigen of a monoclonal antibody that is specific for cells human carcinoma the colon monosialoganglioside as determined by direct binding to thin-layer chromatograms total lipid extracts tissues. Binding detected autoradiography after application iodine-125-labeled F(ab′) 2 rabbit immunoglobulin G antibodies mouse immunoglobulins.
Glycans are critical to every facet of biology and medicine, from viral infections embryogenesis. Tools study glycans rapidly evolving; however, the majority our knowledge is deeply dependent on binding by glycan proteins (e.g., lectins). The specificities lectins, which often naturally isolated proteins, have not been well-defined, making it difficult leverage their full potential for analysis. Herein, we use a combination machine learning algorithms expert annotation define lectin...
Squirrel monkeys have high circulating cortisol to compensate for expression of low-affinity glucocorticoid receptors (GRs). We demonstrated that the FK506-binding immunophilin FKBP51 is elevated in squirrel monkey lymphocytes (SML) and, preliminary studies, shown inhibitory GR binding. In this report, we unequivocal cause resistance SML following ways: 1) FK506 increased binding cytosol from a concentration-dependent manner, an effect reproduced by rapamycin but not cyclosporin A. The...
The assembly of progesterone receptor (PR) heterocomplexes in vitro involves at least eight components the molecular chaperone machinery, and as earlier reports have shown, these proteins exhibit complex, dynamic, but ordered, interactions with one another PR. Using selective hsp90 binding agent geldanamycin (GA), we found that PR can be arrested a previously observed intermediate step. Like mature complexes, complexes contain hsp90, they differ from by presence hsp70, p60, p48 absence...
A cell-free system was used to examine heat shock protein 90 (hsp90)-progesterone receptor (PR) binding at near physiological conditions. Four major findings are presented: 1) hsp90 is required maintain ligand by PR elevated temperatures; 2) hsp70 and shock-related p60 components of an intermediate assembly complex that precedes formation a mature hsp90-PR complex; 3) complexes in steady state assembly/disassembly cycle (t1/2, 5 min); 4) dissociation not accelerated after progesterone...
Immunoaffinity purification of hsp90 from chick oviduct cytosol reveals two major proteins, hsp70 and a 60-kDa protein (p60), copurifying with hsp90. A similar result is obtained when immunoaffinity purified liver brain cytosols, avian fibroblasts, rabbit reticulocyte lysate. This p60 the same previously identified in certain assembly complexes progesterone receptor generated cell-free reconstitution system. Tryptic cyanogen bromide peptide fragments were gel-purified p60, partial N-terminal...
The ability to bind immunosuppressive drugs such as cyclosporin and FK506 defines the immunophilin family of proteins, FK506-binding proteins form FKBP subfamily immunophilins. Some FKBPs, notably FKBP12 (the 12-kDa protein), have defined roles in regulating ion channels or cell signaling, well established structures. Other especially larger ones, participate important biological processes, but their exact structural bases for these are poorly defined. FKBP51 51-kDa FKBP) associates with...
Progesterone receptor complexes were purified from crude cytosol in a rapid, gentle, one-step procedure using anti-receptor monoclonal antibodies covalently attached to an agarose resin. Five nonreceptor proteins specifically co-purified with unactivated avian progesterone receptor; these had molecular masses of approximately 90, 70, 54, 50, and 23 kDa. The 90- 70-kDa have been previously identified as the 90-kDa heat shock protein member family, respectively. 54-, 50-, 23-kDa not described...
Embryo implantation in the uterus is a critical step mammalian reproduction, requiring preparation of receptive to blastocyst implantation. Uterine receptivity, also known as window implantation, lasts for limited period, and it during this period blastocysts normally implant. Ovarian steroid hormones estrogen progesterone (P 4 ) are primary regulators process. The immunophilin FKBP52 serves cochaperone hormone nuclear receptors govern appropriate action target tissues. Here we show role...
In the absence of stress, human heat shock factor 1 (hHSF1) is in its unactivated form. hHSF1 polypeptide a dynamic heterocomplex with Hsp90 and incapable specifically binding DNA. When cells are stressed, assembly disrupted. Unbound homotrimerizes, acquires DNA activity, concentrates nucleus, but remains transcriptionally inactive. A subsequent reaction converts this inactive, trimeric form into active, hyperphosphorylated transcription factor. Subsequent to stressful event, deactivated...
Previous studies on the assembly of progesterone receptor (PR) complexes in vitro have suggested that PR is a dynamic, ordered process involving at least eight nonreceptor proteins. One these proteins, p60, appears transiently during and not component functionally mature complexes. In present study we observe monoclonal antibody specific for p60 can, one hand, inhibit formation containing heat shock protein 90 (hsp90), p23, immunophilins and, other, enhance recovery early hsp70 Hip (p48)....
A cDNA for human FKBP51 has been cloned and sequenced, protein products have expressed in both vitro bacterial systems. The deduced amino acid sequence is 90% identical to sequences of recently described murine proteins 55% the FKBP52. Human mRNA a wide range tissues, peptidylprolyl isomerase activity that inhibited by FK506 but not cyclosporine. same as previously progesterone receptor-associated immunophilin that, similar FKBP52 cyclophilin 40, an Hsp90-binding appears functionally mature...
The protein composition of the avian progesterone receptor was analyzed by immune isolation complexes and gel electrophoresis isolated proteins. Nonactivated cytosol in association with 90-kilodalton (kDa) heat shock protein, hsp90, as has been described previously. A 70-kDa also observed shown Western immunoblotting to react an antibody specific protein. Thus, two receptor-associated proteins are identical, or closely related, When species, B, were separately absence hormone, both obtained...