A5101569400- Glycosylation and Glycoproteins Research
- Proteoglycans and glycosaminoglycans research
- Carbohydrate Chemistry and Synthesis
- Monoclonal and Polyclonal Antibodies Research
- Natural product bioactivities and synthesis
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Biological Activity of Diterpenoids and Biflavonoids
- Microbial Metabolites in Food Biotechnology
- Platelet Disorders and Treatments
- Endoplasmic Reticulum Stress and Disease
- Toxin Mechanisms and Immunotoxins
- Bacterial Genetics and Biotechnology
- Phytochemistry and Biological Activities
- Bioactive natural compounds
- Advanced biosensing and bioanalysis techniques
- Redox biology and oxidative stress
- SARS-CoV-2 detection and testing
- Heat shock proteins research
- RNA and protein synthesis mechanisms
- Analytical chemistry methods development
- Selenium in Biological Systems
- Infant Nutrition and Health
- CRISPR and Genetic Engineering
- Mycotoxins in Agriculture and Food
Tianjin Medical University
2023-2025
New England Biolabs (United States)
2017-2024
Gansu Academy of Sciences
2011-2024
Gansu University of Traditional Chinese Medicine
2022
Academy of Medical Sciences
2022
China Agricultural University
2019
Gansu Provincial Hospital
2019
Gansu Provincial Hospital of TCM
2015
Boston University
2009-2013
Huazhong Agricultural University
2013
Glycosylation is a topic of intense current interest in the development biopharmaceuticals because it related to drug safety and efficacy. This work describes results an interlaboratory study on glycosylation Primary Sample (PS) NISTmAb, monoclonal antibody reference material. Seventy-six laboratories from industry, university, research, government, hospital sectors Europe, North America, Asia, Australia submitted total 103 reports glycan distributions. The principal objective this was...
The functions of heparan sulfate (HS) depend on the expression structural domains that interact with protein partners. Glycosaminoglycans (GAGs) exhibit a high degree polydispersity in their composition, chain length, sulfation, acetylation, and epimerization patterns. It is essential for understanding GAG biochemistry to produce detailed information as function spatial temporal factors biological systems. Toward this end, we developed set procedures extract GAGs from various rat organ...
A key challenge to investigations into the functional roles of glycosaminoglycans (GAGs) in biological systems is difficulty achieving sensitive, stable, and reproducible mass spectrometric analysis. GAGs are linear carbohydrates with domains that vary extent sulfation, acetylation, uronic acid epimerization. It particular importance determine spatial temporal variations GAG domain structures tissues. In order analyze from tissue, it useful couple MS an on-line separation system. The...
Abstract Rhizobia in legume root nodules fix nitrogen symbiosomes, organelle-like structures which a membrane from the host plant surrounds symbiotic bacteria. However, components that transport plant-synthesized lipids to symbiosome remain unknown. This study identified and functionally characterized Chinese milk vetch (Astragalus sinicus) lipid transfer protein AsE246, is specifically expressed nodules. It was found AsE246 can bind vitro. More importantly, digalactosyldiacylglycerol vivo....
Characterization of mucin-type O-glycans linked to serine/threonine glycoproteins is technically challenging, in part, due a lack effective enzymatic tools that enable their analysis. Recently, several O-glycan-specific endoproteases can cleave the protein adjacent appended glycan have been described. Despite significant progress understanding biochemistry these enzymes, known O-glycoproteases specificity constraints, such as inefficient cleavage bearing sialylated O-glycans, high...
Reactive oxygen species exacerbate nonalcoholic steatohepatitis (NASH) by oxidizing macromolecules; yet how they promote NASH remains poorly understood. Here, we show that peroxidase activity of global hepatic peroxiredoxin (PRDX) is significantly decreased in NASH, and palmitic acid (PA) binds to PRDX1 inhibits its activity. Using three genetic models, demonstrate protects against male mice. Mechanistically, suppresses STAT signaling mitochondrial function scavenging hydrogen peroxide,...
Abstract A method for selective and comprehensive enrichment of N-linked glycopeptides was developed to facilitate detection micro-heterogeneity N-glycosylation. The takes advantage the inherent properties Fbs1, which functions within ubiquitin-mediated degradation system recognize common core pentasaccharide motif (Man3GlcNAc2) glycoproteins. We show that Fbs1 is able bind diverse types glycomolecules; however, wild-type preferentially binds high-mannose-containing glycans. identified...
Heparan sulfate (HS) serves as a cell-surface co-receptor for growth factors, morphogens, and chemokines. These HS protein binding events depend on the fine structure distribution of domains along an chain. A given domain can vary in terms uronic acid epimer, N- O-sulfate, N-acetate content. The most highly sulfated regions chains, N-sulfated (NS) domains, play prominent roles binding. We have analyzed oligosaccharides from various mammalian sources provide evidence that NS residing at...
Glycosaminoglycans ( GAG s) vary widely in disaccharide and oligosaccharide content a tissue‐specific manner. Nonetheless, there are common structural features, such as the presence of highly sulfated non‐reducing end domains on heparan sulfate HS ) chains. Less clear patterns expression s specific cell types. Leukocytes known to express primarily chondroitin CS type. However, little is regarding properties structures chains, their variability among normal subjects, changes structure...
Glycosaminoglycans (GAGs) are found in intracellular granules, cell surfaces, and extracellular matrices a spatially temporally regulated fashion, constituting the environment for cells to interact, migrate, proliferate. Through binding with great number of proteins, GAGs regulate many facets biological processes from embryonic development normal physiological functions. have been shown be involved pathologic changes immunological responses including cancer metastasis inflammation. Past...
Peroxiredoxin 1 (PRDX1) is a peroxidase and guards against oxidative stress by scavenging intracellular peroxides, whereas it also has been shown to stimulate inflammatory response functioning as chaperone protein. The potential in vivo link between PRDX1's activity its pro-inflammatory remains elusive. We generated peroxidase-dead PRDX1 variant mice mutating peroxidatic cysteine at 52 (Cys52) serine, here referred PRDX1Cys52Ser. Trx-TrxR-NADPH coupled assay was applied evaluate the of...
Tularemia is a severe infectious disease in humans caused by the Gram-negative bacterium Francisella tularensis (Ft). Because of its low dose, high mortality rate, and threat large-scale dissemination weaponized form, development vaccines immunotherapeutics against Ft essential. lipopolysaccharide (LPS), which contains linear graded-length saccharide component O-antigen (OAg) attached to core oligosaccharide, has been reported as protective antigen. Purification LPS saccharides defined...
Glycosaminoglycans (GAGs) isolated from brittlestars, Echinodermata class Ophiuroidea, were characterized, as part of attempts to understand the evolutionary development these polysaccharides. A population chondroitin sulfate/dermatan sulfate (CS/DS) chains with a high overall degree sulfation and hexuronate epimerization was major GAG found, whereas heparan (HS) below detection level. Enzymatic digestion different lyases revealed exceptionally proportions di- trisulfated CS/DS...
Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus spp. and Penicillium poses threat to food safety. Biodegradation may be promising strategy for reducing the OTA contamination in future. In this study, niger strain W-35 was isolated from cereals studied its ability degrade OTA. Results showed that supernatant of could both vitro commercial feeds after incubation at 37 °C 12 h 78.0 37.0%, respectively. α (OTα) assayed as degradation product HPLC-FLD. Furthermore, an enzyme specific...
Background The Sulfs are a family of endosulfatases that selectively modify the 6O-sulfation state cell-surface heparan sulfate (HS) molecules. serve as modulators cell-signaling events because changes they induce alter cell surface co-receptor functions HS chains. A variety studies have been aimed at understanding how structure, and many these utilize Sulf knockout lines source for used in experiments. However, genetic manipulation has shown to expression levels biosynthetic enzymes, cases...
Exoglycosidases are often used for detailed characterization of glycan structures. Bovine kidney α-fucosidase is commonly to determine the presence core α1-6 fucose on N-glycans, an important modification glycoproteins. Recently, several studies have reported that removal α1-6-linked from N-glycans labeled with reactive N-hydroxysuccinimide carbamate fluorescent labels 6-aminoquinolyl-N-hydroxysuccinimidylcarbamate (AQC) and RapiFluor-MS severely impeded. We report here cloning, expression...
Cedrus deodara (Roxb.) Loud (normally called as deodar), one out of four species in the genus Cedrus, exhibits widely biological activities. The total lignans from pine needles (CTL) were extracted. aim study was to investigate anticancer potential CTL on A549 cell line.We extracted by ethanol and assessed cytotoxicity CCK-8 method. Cell cycle apoptosis detected a FACS Verse Calibur flow cytometry.The means hot refluxing content about 55.77%. By assays, inhibited growth cells dose-dependent...
Antioxidants remove free radicals and inhibit the oxidation of oxygen-sensitive substances, which are great significance in disease prevention food preservative. Therefore, it is to establish a convenient, efficient universal method for screening evaluating antioxidant activity. In this study, Nitrogen-doped Graphene (N-G) with high conductivity Chitosan (CS) good film forming stability were used as electrode substrate materials. And ds-DNA/N-G@CS/GCE electrochemical biosensor rapid...
Objective: To investigate the effects of different prescription compositions traditional Chinese medicine and its extraction methods compound formula extracts on hypoxia tolerance in mice, order to preferably select their preparation methods. Methods: Male BALB/c mice were randomly divided into 6 groups: blank control group, danshen Rhodiola Rosea alcohol-water extract group (Rhodiola rosea, Astragali Radix, Polygonati Rhizoma, Lycii Fructus), water Astragalus (Astragali Fructus) 30 each...
Patrinia scabra Bunge is a well-known herbal medicine for its favorable treatment on inflammatory diseases owing to effective ingredients, in which iridoid glycoside plays an extremely significant role. This article aimed improve the content of total glycosides crude extract through series optimization extraction procedure. Moreover, considering that both pain and inflammation are two correlated responses triggered response injury, irritants or pathogen, investigated anti-inflammatory...
The specificities of glycosaminoglycan (GAG) modification enzymes, particularly sulfotransferases, and the locations concentrations these enzymes in Golgi apparatus give rise to mature GAG polysaccharides that bind protein ligands. We studied substrate sulfotransferases with a stable isotopically labeled donor substrate, 3′-phosphoadenosine-5′-phosphosulfate. sulfate incorporated by vitro sulfation using recombinant was easily distinguished from those previously present on chains mass...