A5047633562- Glycosylation and Glycoproteins Research
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Monoclonal and Polyclonal Antibodies Research
- Machine Learning in Bioinformatics
- Galectins and Cancer Biology
- Genomics and Phylogenetic Studies
- Carbohydrate Chemistry and Synthesis
- Peptidase Inhibition and Analysis
- Bioinformatics and Genomic Networks
- RNA and protein synthesis mechanisms
- Advanced Chemical Sensor Technologies
- Enzyme Structure and Function
- Advanced Glycation End Products research
- Protease and Inhibitor Mechanisms
- Lipid metabolism and biosynthesis
- Ubiquitin and proteasome pathways
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Ginseng Biological Effects and Applications
- Autophagy in Disease and Therapy
- Microbial Metabolic Engineering and Bioproduction
- Protein purification and stability
Korea Basic Science Institute
2013-2022
Chungnam National University
2012-2021
IONICS Mass Spectrometry (Canada)
2015
Yonsei University
2002-2012
Hannam University
2010
Korea Research Institute of Bioscience and Biotechnology
1997-2010
Korea Institute of Science and Technology
2010
Korea University of Science and Technology
2010
Kyungpook National University
2010
Waters (United States)
2010
Abstract Disorders of autophagy, a key regulator cellular homeostasis, cause number human diseases. Due to the role autophagy in metabolic dysregulation, there is need identify regulators as therapeutic targets. To address this need, we conducted an phenotype-based screen and identified natural compound kaempferide (Kaem) enhancer. Kaem promoted through translocation transcription factor EB (TFEB) without MTOR perturbation, suggesting it safe for administration. Moreover, accelerated lipid...
Abstract Glycoproteomics is a powerful yet analytically challenging research tool. Software packages aiding the interpretation of complex glycopeptide tandem mass spectra have appeared, but their relative performance remains untested. Conducted through HUPO Human Initiative, this community study, comprising both developers and users glycoproteomics software, evaluates solutions for system-wide analysis. The same spectrometry based datasets from human serum were shared with participants team...
Abstract Proteomic analysis of Pseudomonas putida KT2440 cultured in monocyclic aromatic compounds was performed using 2‐DE/MS and cleavable isotope‐coded affinity tag (ICAT) to determine whether proteins involved compound degradation pathways were altered as predicted by genomic (Jiménez et al. , Environ Microbiol. 2002, 4 824–841). Eighty unique identified or MS/MS from P. the presence six different organic compounds. Benzoate dioxygenase (BenA, BenD) catechol 1,2‐dioxygenase (CatA)...
The objective of the international Chromosome-Centric Human Proteome Project (C-HPP) is to map and annotate all proteins encoded by genes on each human chromosome. C-HPP consortium was established organize a collaborative network among research teams responsible for protein mapping individual chromosomes identify compelling biological genetic mechanisms influencing colocated their products. aims foster development proteome analysis integration findings from related molecular -omics...
Glycosylation is a topic of intense current interest in the development biopharmaceuticals because it related to drug safety and efficacy. This work describes results an interlaboratory study on glycosylation Primary Sample (PS) NISTmAb, monoclonal antibody reference material. Seventy-six laboratories from industry, university, research, government, hospital sectors Europe, North America, Asia, Australia submitted total 103 reports glycan distributions. The principal objective this was...
Human glycoproteins exhibit enormous heterogeneity at each N-glycosite, but few studies have attempted to globally characterize the site-specific structural features. We developed Integrated GlycoProteome Analyzer (I-GPA) including mapping system for complex N-glycoproteomes, which combines methods tandem mass spectrometry with a database search and algorithmic suite. Using an N-glycopeptide that we constructed, created novel scoring algorithms decoy glycopeptides, where 95 N-glycopeptides...
Significance Deciphering molecular mechanisms at the glycome level in mammalian brain remains a missing piece of puzzle neuroscience due to intrinsic complexity glycosylation and lack analytical tools. Here, we uncovered variation diversity expression human mouse samples according spatial temporal differences. We further constructed comprehensive synthesis map using glycans structurally elucidated by LC-MS/MS found strong evidence on conservation developmental divergence prefrontal cortex...
Human plasma is the most clinically valuable specimen, containing not only a dynamic concentration range of protein components, but also several groups high-abundance proteins that seriously interfere with detection low-abundance potential biomarker proteins. To establish high-throughput method for efficient depletion and subsequent fractionation, prior to molecular analysis proteins, we explored how coupled immunoaffinity columns, commercially available as multiple affinity removal columns...
The various components of crude oil were structurally resolved using an atmospheric-pressure solids analysis probe (ASAP) coupled with ion mobility mass spectrometry (IM-MS). An ASAP source was used to broadly fractionate compounds according their boiling points, thereby simplifying the resulting spectra for easier data interpretation. m/z-mobility plots obtained by IM-MS could be find structural relationship between molecules. That demonstrated from a homologous series compounds, differing...
High mobility group box 1 protein (HMGB1), a nuclear protein, can be translocated to the cytoplasm and secreted in colon cancer cells. However, diagnostic significance of HMGB1 has not been evaluated colorectal carcinomas. For this purpose, we have screened expression secretion 10 cell lines control line found that was detected culture medium. To evaluate value HMGB1, performed an enzyme-linked immunosorbent assay measure levels compared them carcinoembryonic antigen (CEA) serum samples 219...
Posttranslational acetylation of proteins regulates many diverse functions, including DNA recognition, protein−protein interaction, and protein stability. The identification enzymes that regulate has revealed broader use this modification than was previously suspected. In study, we describe a method for identifying at lysine residues by analysis digested using HPLC/ESI-MS with new modification-specific marker ion. Collision-induced dissociation capillary or nano-LC/ESI-TOF-MS used to obtain...
New crown-appended cholesterol-based organogelators 1-3, which have one or two cholesterol skeletons as a chiral aggregate-forming site, amino groups an acidic proton binding and crown moiety cation were synthesized, the gelation ability was evaluated in organic solvents. These gelators could gelatinize several solvents under 1.0 wt %, indicating that 1-3 act versatile gelator of various We observed CD spectra acetic acid propionic gels to characterize aggregation mode organogel system. In...
Variations in glycosylation levels or the glycoprofile of a certain glycoprotein tumor-related sera have been widely reported and can be used as means differentiation. However, quantitative mass analysis glycoproteins is difficult because their high structural complexity low sensitivity glycopeptides. Therefore, more powerful technologies are required for discovery these potential biomarkers. Tissue inhibitor metalloproteinase 1 (TIMP1), typically present at concentration serum, known to...
N-Acetylglucosaminyltransferase-V (GnT-V) has been reported to be up-regulated in invasive/metastatic cancer cells, but a comprehensive understanding of how the transferase correlates with potential is not currently available. Through glycomics approach, we identified 30 proteins, including tissue inhibitor metalloproteinase-1 (TIMP-1), as target protein for GnT-V human colon cell WiDr. TIMP-1 was aberrantly glycosylated characterized by addition beta1,6-N-acetylglucosamine,...
Albumin, an abundant plasma protein with multifunctional properties, is mainly synthesized in the liver. Albumin has been implicated Alzheimer's disease (AD) since it can bind to and transport amyloid beta (Abeta), causative agent of AD; albumin also a potent inhibitor Abeta polymerization. Despite evidence non-hepatic transcription many tissues including kidney pancreas, synthesis at level rarely confirmed. In pilot phase study Human Brain Proteome Project, we found that microglial cells...
Abstract Alzheimer’s disease (AD) is the most common form of dementia; however, mechanisms and biomarkers remain unclear. Here, we examined hippocampal CA4 dentate gyrus subfields, which are less studied in context AD pathology, post-mortem control tissue to identify possible biomarkers. We performed mass spectrometry-based proteomic analysis combined with label-free quantification for identification differentially expressed proteins. identified 4,328 proteins, 113 showed more than 2-fold...
The drug FK506 (tacrolimus, fujimycin) exerts its immunosuppressive effects by regulating the nuclear factor of activated T-cell (NFAT) family transcription factors. However, also exhibits neuroprotective effects, but direct target proteins that mediate these have not been determined. To identify responsible for FK506's affinity responsive stability (DARTS) method was performed using label-free FK506, and LC–MS/MS analysis FK506-treated proteome performed. Using DARTS analyses in combination...
Acinetobacter baumannii is a Gram-negative, nonmotile aerobic bacterium that has emerged as an important nosocomial pathogen. Multidrug-resistant (MDR) A. difficult to treat with antibiotics, and treatment failure in infected patients of great concern clinical settings. To investigate proteome regulation under antibiotic stress conditions, quantitative membrane proteomic analyses MDR strain cultured subminimal inhibitory concentrations tetracycline imipenem were performed using combination...
An important goal of the Human Proteome Organization (HUPO) Chromosome-centric Project (C-HPP) is to correctly define number canonical proteins encoded by their cognate open reading frames on each chromosome in human genome. When identified with high confidence protein evidence (PE), such are termed PE1 online database resource, neXtProt. However, that have not been unequivocally at level but other suggestive existence (PE2–4) missing (MPs). The MPs has reduced from 5511 2012 2186 2018...
Glycoprotein conformations are complex and heterogeneous. Currently, site-specific characterization of glycopeptides is a challenge. We sought to establish an efficient method N-glycoprotein using mass spectrometry (MS). Using alpha-1-acid glycoprotein (AGP) as model N-glycoprotein, we identified its tryptic N-glycopeptides examined the data reproducibility in seven laboratories running different LC-MS/MS platforms. used three test samples one blind sample evaluate instrument performance...