A5100417185- Circadian rhythm and melatonin
- RNA modifications and cancer
- Signaling Pathways in Disease
- RNA and protein synthesis mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Spaceflight effects on biology
- Histone Deacetylase Inhibitors Research
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Microbial Metabolic Engineering and Bioproduction
- Peptidase Inhibition and Analysis
- Reproductive System and Pregnancy
- Galectins and Cancer Biology
- HIV Research and Treatment
- Genomics and Chromatin Dynamics
- Wnt/β-catenin signaling in development and cancer
- Nerve injury and regeneration
- Wireless Communication Networks Research
- Adipose Tissue and Metabolism
- Bioinformatics and Genomic Networks
- RNA Research and Splicing
- Neuroscience and Neuropharmacology Research
- Immune Cell Function and Interaction
- Trace Elements in Health
- Extracellular vesicles in disease
City University of Hong Kong
2019-2024
National Cancer Center
2023
Seoul National University
2003-2022
Chonnam National University Hwasun Hospital
2022
Korea University
2022
Johns Hopkins University
2022
Dongshin University
2022
City University of Hong Kong, Shenzhen Research Institute
2020-2021
Korea Basic Science Institute
2016-2021
Chosun University
2009-2020
Significance Circadian clocks are endogenous molecular oscillators that drive daily rhythms of physiology and behavior. The mammalian clock, intrinsic to most cells tissues, is built on a conserved negative feedback loop in which heterodimeric transcription factor, circadian locomotor output cycles kaput (CLOCK)-brain, muscle Arnt-like 1 (BMAL1), drives the its specific inhibitor proteins. This study shows CLOCK-BMAL1 associated with clock-driven, rhythmically expressed coactivator protein,...
Although Daxx (death-associated protein) was first reported to mediate the apoptotic signal from Fas JNK in cytoplasm, other data suggested that is mainly located nucleus as a transcriptional regulator. Here, we demonstrated cellular localization of could be determined by relative concentration proapoptotic kinase, apoptosis signal-regulating kinase 1 (ASK1) using immunofluorescence and reporter assay. ASK1 sequestered cytoplasm inhibited repressive activity transcription. In addition, bound...
Mammalian tRNA synthetases form a macromolecular complex with three nonenzyme factors: p43, p38, and p18. Here we introduced mutation within the mouse p38 gene to understand its functional significance for formation of multi-tRNA synthetase complex. The was completely disintegrated by deficiency p38. In addition, protein levels catalytic activities component enzymes cofactors were severely decreased. A partial truncation polypeptide separated associated components into different subdomains....
The drug FK506 (tacrolimus, fujimycin) exerts its immunosuppressive effects by regulating the nuclear factor of activated T-cell (NFAT) family transcription factors. However, also exhibits neuroprotective effects, but direct target proteins that mediate these have not been determined. To identify responsible for FK506's affinity responsive stability (DARTS) method was performed using label-free FK506, and LC–MS/MS analysis FK506-treated proteome performed. Using DARTS analyses in combination...
<i>Background/Aims:</i> Apoptosis contributes to cyclosporine (CsA)-induced renal cell death. This study tested the effects of CsA-induced endoplasmic reticulum (ER) stress on apoptotic death in an experimental model chronic CsA nephropathy. <i>Methods:</i> (15 mg/kg per day) was given rats for 7 or 28 days. The ER response evaluated with BiP expression, and proapoptotic assessed CHOP caspase 12 expression. structure by transmission electron microscopy, detected TUNEL...
AIMP1/p43 is known as a cytokine working in the control of angiogenesis, inflammation, and wound healing. Here we report its enrichment pancreatic α cells glucagon-like hormonal activity. AIMP1 secreted from pancreas upon glucose starvation. Exogenous infusion increased plasma levels glucose, glucagon, fatty acid, AIMP1-deficient mice showed reduced compared with wild-type under fasting conditions. Thus, plays role homeostasis.
Oligodendrogliopathy, microglial infiltration, and lack of remyelination are detected in the brains patients with multiple sclerosis accompanied by high levels transcription factor p53. In this study, we used cuprizone model demyelination, characterized oligodendrogliopathy to define effect <i>p53</i> inhibition. Myelin preservation, decreased recruitment, gene expression were observed mice lacking or receiving systemic administration p53 inhibitor pifithrin-α, compared untreated controls....
Circadian clocks, generating daily rhythms in biological processes, maintain homeostasis physiology, so clock alterations are considered detrimental. Studies brain pathology support this by reporting abnormal circadian phenotypes patients, but restoring the abnormalities light therapy shows no dramatic effects. Recent studies on glial clocks report complex effects of altered showing their beneficial repairs. However, how neuronal respond to is elusive. This study that BMAL1, a core reduces...
The Notch signaling pathway appears to perform an important function in a wide variety of organisms and cell types. In our present study, we provide evidence that UV irradiation-induced Tip60 proteins reduced Notch1 activity marked degree. Accumulated suppresses transcriptional via the dissociation Notch1-IC-CSL complex. binding between endogenous Notch1-IC radiation-exposed cells was verified this study by coimmunoprecipitation. Interestingly, physical interaction with occurs more profound...
Pin1, a conserved eukaryotic Peptidyl-prolyl cis/trans isomerase, has profound effects on numerous key-signaling molecules, and its deregulation contributes to disease, particularly cancer. Although Pin1-mediated prolyl isomerization is an essential novel regulatory mechanism for protein phosphorylation, little known about the upstream signaling pathway(s) that regulates Pin1 activity. Here, we identify MAP3K-related serine–threonine kinase (the gene encoding COT/Tpl2) as responsible...
The general consensus is that immune cells are exposed to physiological hypoxia in vivo (PhyO2, 2-5% P(O2)). However, functional studies of B hypoxic conditions sparse. Recently, we reported the expression mouse TASK-2, a member pH-sensitive two-pore domain K(+) channels with background activity. In this study, investigated response sustained PhyO2 (sustained [SH], 3% P(O2) for 24 h) WEHI-231 cells. SH induced voltage-independent conductance (SH-K(bg)) and hyperpolarized membrane potential....
Circadian clocks are endogenous oscillators that generate cell-autonomous rhythms govern cellular processes and synchronized by external cues in the local macro- micro-environments. Demyelination, a common brain pathology with variable degrees of recovery, changes microenvironment via damaged myelin activation glial cells. How these microenvironmental affect circadian what consequences is mostly unknown. Here, we show within demyelinating lesions, astrocyte produce Wnt inhibitors SFRP1...