A5063635262- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Proteoglycans and glycosaminoglycans research
- Advanced Proteomics Techniques and Applications
- Galectins and Cancer Biology
- Mycobacterium research and diagnosis
- Genomics and Phylogenetic Studies
- Monoclonal and Polyclonal Antibodies Research
- Mass Spectrometry Techniques and Applications
- Polysaccharides and Plant Cell Walls
- Trypanosoma species research and implications
- Invertebrate Immune Response Mechanisms
- Enzyme Production and Characterization
- Redox biology and oxidative stress
- Protein Tyrosine Phosphatases
- Immunotherapy and Immune Responses
- Tuberculosis Research and Epidemiology
- Viral gastroenteritis research and epidemiology
- Nitric Oxide and Endothelin Effects
- Transgenic Plants and Applications
- Cancer Research and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Ubiquitin and proteasome pathways
- Escherichia coli research studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
National Taiwan University
2015-2025
Institute of Biological Chemistry, Academia Sinica
2016-2025
National Institutes of Natural Sciences
2021-2023
Academia Sinica
2008-2021
National Yang Ming Chiao Tung University
2017
Shura Council
2015
Knowledge Unlatched (Germany)
2015
Genomics Research Center, Academia Sinica
2006-2013
Imperial College London
1990-2012
National Tsing Hua University
2011-2012
Abstract Extracellular interaction between programmed death ligand-1 (PD-L1) and cell protein-1 (PD-1) leads to tumour-associated immune escape. Here we show that the immunosuppression activity of PD-L1 is stringently modulated by ubiquitination N -glycosylation. We glycogen synthase kinase 3β (GSK3β) interacts with induces phosphorylation-dependent proteasome degradation β-TrCP. In-depth analysis N192, N200 N219 glycosylation suggests antagonizes GSK3β binding. In this regard, only...
Mass spectrometry (MS) of glycoproteins is an emerging field in proteomics, poised to meet the technical demand for elucidation structural complexity and functions oligosaccharide components molecules. Considering divergence mass spectrometric methods employed analysis recent publications, it necessary establish standards demonstrate capabilities. In present study Human Proteome Organisation (HUPO) Disease Glycomics/Proteome Initiative (HGPI), same samples transferrin immunoglobulin-G were...
Protein glycosylation is an important posttranslational process, which regulates protein folding and functional expression. Studies have shown that abnormal in tumor cells affects cancer progression malignancy. In the current study, we identified sialylated proteins using alkynyl sugar probe two different lung cell lines, CL1-0 CL1-5 with distinct invasiveness derived from same parental line. Among proteins, epidermal growth factor receptor (EGFR) was chosen to understand effect of...
Enriched PD-L1 expression in cancer stem-like cells (CSCs) contributes to CSC immune evasion. However, the mechanisms underlying enrichment CSCs remain unclear. Here, we demonstrate that epithelial-mesenchymal transition (EMT) enriches by EMT/β-catenin/STT3/PD-L1 signaling axis, which EMT transcriptionally induces N-glycosyltransferase STT3 through β-catenin, and subsequent STT3-dependent N-glycosylation stabilizes upregulates PD-L1. The axis is also utilized general cell population, but it...
Fertilization in humans is initiated by binding of spermatozoa to a selectin ligand on the egg’s extracellular matrix.
Recent cases of avian influenza H5N1 and the swine-origin 2009 H1N1 have caused a great concern that global disaster like 1918 pandemic may occur again. Viral transmission begins with critical interaction between hemagglutinin (HA) glycoprotein, which is on viral coat influenza, sialic acid (SA) containing glycans, are host cell surface. To elucidate role HA glycosylation in this important interaction, various defined glycoforms were prepared, their binding affinity specificity studied by...
Abstract Glycoproteomics is a powerful yet analytically challenging research tool. Software packages aiding the interpretation of complex glycopeptide tandem mass spectra have appeared, but their relative performance remains untested. Conducted through HUPO Human Initiative, this community study, comprising both developers and users glycoproteomics software, evaluates solutions for system-wide analysis. The same spectrometry based datasets from human serum were shared with participants team...
Most membrane proteins are modified by covalent addition of complex sugars through N- and O-glycosylation. Unlike proteins, glycans do not typically adopt specific secondary structures remain very mobile, shielding potentially large fractions protein surface. High glycan conformational freedom hinders complete structural elucidation glycoproteins. Computer simulations may be used to model glycosylated but require hundreds thousands computing hours on supercomputers, thus limiting routine...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA new interpretation of the structure mycolyl-arabinogalactan complex Mycobacterium tuberculosis as revealed through characterization oligoglycosylalditol fragments by fast-atom bombardment mass spectrometry and 1H nuclear magnetic resonance spectroscopyGurdyal S. Besra, Kay-Hooi Khoo, Michael R. McNeil, Anne Dell, Howard Morris, Patrick J. BrennanCite this: Biochemistry 1995, 34, 13, 4257–4266Publication Date (Print):April 4, 1995Publication...
Previous studies have demonstrated that the nonreducing termini of lipoarabinomannan (LAM) from Mycobacterium tuberculosis are extensively capped with mannose residues, whereas those a fast growing sp., once thought to be an attenuated strain M. tuberculosis, not. The noncapped LAM, termed AraLAM, is known more potent than mannose-capped LAM (ManLAM) in inducing functions associated macrophage activation. Using combination chemical and enzymatic approaches coupled atom bombardment-mass...
A new class of inositol phosphates containing energy-rich pyrophosphoryl residues has been characterized. D/L-1-Diphosphoinositol pentakisphosphate(s) and D/L-bis-(1,4)-diphosphoinositol tetrakisphosphate(s) are present as soluble ionic species in the cytosol amoebae (Dictyostelium discoideum) at concentrations range 0.05-0.25 mM. These compounds rapidly metabolized intact cells can be synthesized cell lysates from myo-inositol hexakisphosphate presence ATP. Their phosphomonoester groups...
In this study, we used a systems biology approach to investigate changes in the proteome and metabolome of shrimp hemocytes infected by invertebrate virus WSSV (white spot syndrome virus) at viral genome replication stage (12 hpi) late (24 hpi). At 12 hpi, but not 24 there was significant up-regulation markers several metabolic pathways associated with vertebrate Warburg effect (or aerobic glycolysis), including glycolysis, pentose phosphate pathway, nucleotide biosynthesis, glutaminolysis...
Protein <i>S</i>-nitrosylation mediated by cellular nitric oxide (NO) plays a primary role in executing biological functions cGMP-independent NO signaling. Although appears similar to Cys oxidation induced reactive oxygen species, the molecular mechanism and consequence remain unclear. We investigated structural process of protein-tyrosine phosphatase 1B (PTP1B). treated PTP1B with various donors, including <i>S</i>-nitrosothiol reagents compound-releasing radicals, produce site-specific...
The Human Proteome Organisation Disease Glycomics/Proteome Initiative recently coordinated a multi-institutional study that evaluated methodologies are widely used for defining the N-glycan content in glycoproteins. convincingly endorsed mass spectrometry as technique of choice glycomic profiling discovery phase diagnostic research. present reports extension Initiative's activities to an assessment currently O-glycan analysis. Three samples IgA1 isolated from serum patients with multiple...
Immunotherapies targeting programmed cell death protein 1 (PD-1) and ligand (PD-L1) immune checkpoints represent a major breakthrough in cancer treatment. PD-1 is an inhibitory receptor expressed on the surface of activated T cells that dampens T-cell (TCR)/CD28 signaling by engaging with its PD-L1 cells. Despite clinical success blockade using mAbs, most patients do not respond to treatment, underlying regulatory mechanisms remain incompletely defined. Here we show extensively...
// Cheng-Te Hsiao 1, 2, * , Hung-Wei Cheng 3, Chi-Ming Huang 3 Hao-Ru Li Meng-Hsin Ou Jie-Rong Kay-Hooi Khoo 2 Helen Wenshin Yu 4 Yin-Quan Chen Yang-Kao Wang 5 Arthur Chiou 4, 6 and Jean-Cheng Kuo 7 1 Institute of Biochemical Sciences, National Taiwan University, Taipei 10617, Biological Chemistry, Academia Sinica, 115, Biochemistry Molecular Biology, Yang-Ming 11221, Biophotonics Imaging Research Center, Department Cell Biology Anatomy, Kung Tainan 70101, Biophotonics, Proteomics These...
The MIRAGE (minimum information required for a glycomics experiment) initiative was founded in Seattle, WA, November 2011 order to develop guidelines reporting the qualitative and quantitative results obtained by diverse types of analyses, including conditions techniques that were applied prepare glycans analysis generate primary data along with tools parameters used process annotate this data. These must address broad range issues, as are inherently complex generated using methods, mass...
Feline infectious peritonitis virus (FIPV) is an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment. Here we report 3.3-Å cryoelectron microscopy (cryo-EM) structure of the serotype I FIPV spike (S) protein, which responsible for host recognition and viral entry. Mass spectrometry provided site-specific compositions densely distributed high-mannose complex-type N - glycans account 1/4 total molecular mass; most N-glycans could be visualized by cryo-EM....
Significance Glioblastoma multiforme (GBM) is a deadly brain tumor. More than 50% of patients who suffer from GBM die within 15 mo even received all possible medical treatment. In this study we report that the glycolipid stage-specific embryonic antigen-4 (SSEA-4) highly expressed on surface both cells and specimens. We further demonstrate growth tumor inhibited when anti–SSEA-4 antibody administered to experimental mice, suggesting research proof concept for treatment other SSEA-4 + cancers.