A5102014041- Influenza Virus Research Studies
- Respiratory viral infections research
- Immune Cell Function and Interaction
- Gastrointestinal Bleeding Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Orbital Angular Momentum in Optics
- Laser-Matter Interactions and Applications
- Virus-based gene therapy research
- Advanced optical system design
- Digital Rights Management and Security
- Plasmonic and Surface Plasmon Research
- Optical Polarization and Ellipsometry
- Near-Field Optical Microscopy
- Advanced X-ray Imaging Techniques
- Pediatric health and respiratory diseases
- Pleural and Pulmonary Diseases
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Mechanical and Optical Resonators
- Galectins and Cancer Biology
- SARS-CoV-2 and COVID-19 Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Gastrointestinal Tumor Research and Treatment
Genomics Research Center, Academia Sinica
2019-2024
Fountain Biopharma (Taiwan)
2024
Research Center for Applied Science, Academia Sinica
2020
Institute of Physics, Academia Sinica
2020
Japan Synchrotron Radiation Research Institute
2020
Institute of Chemistry, Academia Sinica
2020
SPring-8
2020
Hokkaido University
2020
National Yang Ming Chiao Tung University
2020
Hospital for Sick Children
2020
Recent cases of avian influenza H5N1 and the swine-origin 2009 H1N1 have caused a great concern that global disaster like 1918 pandemic may occur again. Viral transmission begins with critical interaction between hemagglutinin (HA) glycoprotein, which is on viral coat influenza, sialic acid (SA) containing glycans, are host cell surface. To elucidate role HA glycosylation in this important interaction, various defined glycoforms were prepared, their binding affinity specificity studied by...
Significance Antibodies are important therapeutic agents and have been used for the treatment of many diseases, including infectious inflammatory cancer. The efficacy an antibody is usually determined not only by selectivity affinity toward target but also Fc-glycan structure interacting with Fc receptors on immune cells. This study describes preparation various antibodies different structures as homogeneous glycoforms investigation their effector activities. During this study, it was...
Significance Influenza epidemics continue to be a threat public health, and the recent human cases of avian viruses H5N1, H7N9, H6N1 in Asia raise possibility new disastrous influenza pandemic. Although an effective universal vaccine that can protect from different subtypes or even both type A B is still far reality, our unique findings, showing monosaccharide glycosylated HA induces broader protection against strains, may lead better design does not require frequent updates annual...
Significance Glycosylation is important for the proper folding and function of glycoproteins. However, glycosylation on viral surface protein may mask conserved epitopes causes poor host immune responses protection against infection. To expose more antigenic sites shielded by glycans, we developed a strategy to remove glycans modification traditional egg-based influenza vaccine production procedure. By means immunization with monoglycosylated split virus vaccine, mice were induced broader...
Hemagglutinin (HA) is the immunodominant protein of influenza virus. We previously showed that mice injected with a monoglycosylated A HA (HA mg ) produced cross-strain-reactive antibodies and were better protected than fully glycosylated fg during lethal dose challenge. employed single B-cell screening platform to isolate cross-protective monoclonal antibody (mAb) 651 from immunized A/Brisbane/59/2007 (H1N1) virus (Bris/07). The mAb recognized head domain broad spectrum HAs groups 1 2...
The spatial resolution of x-ray free-electron laser (XFEL) coherent diffraction imaging is currently limited by the fluence XFELs. Here, we clarify this issue systematically studying with a SPring-8 angstrom compact free electron XFEL on gold nanoparticles size from 10 nm to 80 in water solution. patterns obtained single pulses were quantitatively analyzed using small-angle scattering scheme along computer simulations. results show that detectability Au can be described “master curve” as...
This paper proposes a capsule endoscope (CE) with flexible large field of view (FOV) that imitates the compound eyes insects, like those fly. Thirty-one ball lenses are demonstrated to cover 175° viewing area for constructing lens. The lens used in CE is responsible 40° form image on curve plane. A fiber array applied fit plane improve curvature aberration and deliver into an sensor. Such can share aberrations multiple lenses, keep high relative illumination (RI), make adjustment FOV...
This paper proposes a capsule endoscope (CE), based on color multiplexing, to simultaneously record front and side images. Only one lens associated with an X-cube prism is employed catch the view profiles in CE. Three filters polarizers are placed three sides of prism. When objects locate at X-cube's sides, different colors will be caught through proposed recorded image sensor. The multiplexing CE (CMCE) designed field up 210 deg 180 lp/mm resolution under f-number 2.8 overall length 13.323...
The distributions of resonant frequencies in an astigmatic cavity are theoretically confirmed to be analogously equivalent the quantum energy structures two-dimensional commensurate harmonic oscillators. In first part [Opt. Lett.45, 4096 (2020)OPLEDP0146-959210.1364/OL.399251] this two-part series study, lasing modes were verified reveal a variety vortex array structures. Here, second agree very well with Green's functions that correspond bundle Lissajous figures high-order regime.
The spectral shift amplification effect and polarization-controlled shifts are studied using silver (Ag) metal. factor can be doubled by compared with water. solid Ag metal surface also provides orientation freedom for shifts, benefiting data transmission applications in any direction. liquid water interface only limited direction at the Brewster angle. Besides those advantages, higher reflectivity of reflects intensity, which makes signal easier to detect. switch phenomenon that depends on...
An incomplete Freund’s adjuvant elicited an overt pathogenesis in vaccinated mice following the intranasal challenge of A/California/07/2009 (H1N1) virus despite induction a higher specific antibody titer than other adjuvanted formulations. Aluminum hydroxide adjuvants have not induced any pathogenic signs variety formulations with glycolipids. A glycolipid, α-galactosyl ceramide, improved stimulatory effect distinct on anti-influenza response. In contrast to its synthetic analogue C34 was...
The far-field diffraction characteristics of a time-dependent Gaussian pulse incident on slit with central obstruction are studied both theoretically and numerically. It can be shown that the will cause spectrum to compressed compared without an obstruction. Also, diffracted spectral intensity distributions red/blue shifts maximum presented.
A 64-year-old woman presented with chest tightness and progressive dyspnea for 1-week period. She was diagnosed as invasive ductal carcinoma of breast skin, bone bilateral pulmonary metastasis (cT4dN2M1, stage IV; Figure 1a) 2 months prior to this arrival. initially received weekly paclitaxel (80 mg/m2) first-line palliative chemotherapy. Physical examination revealed diminished breath sound in right lung. Chest radiography found pneumothorax …
Abstract Influenza viruses are respiratory pathogens, which cause seasonal outbreaks and result in more than 250,000 annual deaths worldwide. Vaccine inoculation is the most effective way to prevent virus transmission. Our previous study showed that mice injected with monoglycosylated hemagglutinin (HAmg) protein vaccine designed from influenza A produced cross-stain reactive antibodies were protected better fully glycosylated HA (HAfg) after challenge various at lethal dose. Therefore, we...