A5004603450- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- PARP inhibition in cancer therapy
- RNA modifications and cancer
- Advanced Biosensing Techniques and Applications
- Cancer Research and Treatments
- Microfluidic and Capillary Electrophoresis Applications
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- SARS-CoV-2 and COVID-19 Research
- Cancer-related Molecular Pathways
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Glaucoma and retinal disorders
- PI3K/AKT/mTOR signaling in cancer
- Microfluidic and Bio-sensing Technologies
- Cancer, Hypoxia, and Metabolism
- Pancreatic function and diabetes
- Cancer Cells and Metastasis
- Renal and related cancers
- Innovative Microfluidic and Catalytic Techniques Innovation
- Protein Kinase Regulation and GTPase Signaling
- NF-κB Signaling Pathways
- Advanced Proteomics Techniques and Applications
- Nutrition, Genetics, and Disease
Chulalongkorn University
2025
Center for Biologics Evaluation and Research
2020-2024
United States Food and Drug Administration
2020-2024
The University of Texas MD Anderson Cancer Center
2012-2021
China Medical University
2010-2018
The University of Texas Health Science Center at Houston
2006-2016
Asia University
2010-2016
University of Houston
2007-2011
McGill University
2010
China Medical University Hospital
2010
Purpose: To explore whether a cross-talk exists between PARP inhibition and PD-L1/PD-1 immune checkpoint axis, determine blockade of potentiates inhibitor (PARPi) in tumor suppression.Experimental Design: Breast cancer cell lines, xenograft tumors, syngeneic tumors treated with PARPi were assessed for PD-L1 expression by immunoblotting, IHC, FACS analyses. The phospho-kinase antibody array screen was used to the underlying mechanism PARPi-induced upregulation. therapeutic efficacy alone, or...
Transfected Chinese hamster ovary cell lines were developed that expressed equivalent numbers of either normal human receptor or had alanine substituted for Lys-1018 in the ATP-binding domain beta subunit. The mutated was processed into subunits and bound insulin but lacked protein tyrosine kinase activity. Five effects assayed: deoxyglucose uptake, S6 activity, endogenous protein-tyrosine phosphorylation, glycogen synthesis, thymidine uptake. In each case, cells bearing receptors...
The two T cell inhibitory receptors PD-1 and TIM-3 are co-expressed during exhausted differentiation, recent evidence suggests that their crosstalk regulates exhaustion immunotherapy efficacy; however, the molecular mechanism is unclear. Here we show contributes to persistence of
We have previously demonstrated that the human insulin receptor, mutated in ATP-binding domain of beta-subunit, is kinase-defective and fails to mediate multiple post-receptor actions stably transfected Chinese hamster ovary cells (Chou, C.-K., Dull, T. J., Russell, D. S., Gherzi, R., Lebwohl, D., Ullrich, A., Rosen, O. M. (1987) J. Biol. Chem. 262, 1842-1847). This study addresses role protein-tyrosine kinase activity insulin-mediated receptor down-regulation. Although mutant proreceptor...
Significance The ability of printing single-cell arrays with high precision and efficiency, resolution, multiple cell types, maintenance viability function is essential for heterogeneity measurement. It still hard current methods to completely satisfy the above requirements. We report a unique live-cell technique, Block-Cell-Printing, that allows convenient, precise, multiplexed, high-throughput functional arrays. Block-Cell-Printing has minimum turnaround time 0.5 h, maximum resolution 5...
Posttranslational modifications to the intracellular domain of EGFR are known regulate functions; however, extracellular and their effects remain relatively unexplored. Here, we determined that methylation at R198 R200 by protein arginine methyltransferase 1 (PRMT1) enhances binding EGF subsequent receptor dimerization signaling activation. In a mouse orthotopic colorectal cancer xenograft model, expression methylation-defective reduced tumor growth. Moreover, increased sustained activation...
Fullerene compounds have avid reactivity with free radicals and are regarded as ‘radical sponges’. The trimalonic acid derivative of fullerene is one the water-soluble that has been synthesized found to be an effective antioxidant both in vivo vitro. Carboxyfullerene shown treatment Gram-positive -negative infections, although its mode action poorly understood. We determined MIC minimal bactericidal concentration carboxyfullerene for 20 isolates, including Staphylococcus spp., Streptococcus...
Abstract: Carboxyfullerene, a water‐soluble carboxylic acid derivative of fullerene, was investigated as protective agent against iron‐induced oxidative stress in the nigrostriatal dopaminergic system anesthetized rats. Intranigral infusion exclusive carboxyfullerene did not increase lipid peroxidation substantia nigra or deplete dopamine content striatum. Infusion ferrous citrate (iron II) induced degeneration system. An well decreases K + ‐evoked overflow and striatum were observed 7 days...
A phosphoprotein phosphatase that catalyzes the dephosphorylation of cyclic adenosine 3':5'-monophosphate (cAMP)-dependent protein kinase from bovine cardiac muscle has been purified to homogeneity by a modification procedure Brandt et al. (Brandt, H., Capulong, Z.L., and Lee, E. Y. C. (1975) J. Biol. Chem. 250, 8038-8044). Treatment enzyme preparation with ethanol during early stages purification results in activation concomitant reduction molecular weight 30,000. The activated Km for...
Acetylation by ARD1 of an inhibitor mammalian target rapamycin may inhibit cancer growth.
Abstract Molecular trafficking within cells, tissues and engineered three-dimensional multicellular models is critical to the understanding of development treatment various diseases including cancer. However, current tracking methods are either confined two dimensions or limited an interrogation depth ∼15 μm. Here we present a method capable quantifying rapid molecular transport dynamics in highly scattering environments at depths up 200 The system has response time 1 ms with temporal...
Abstract Epithelial-to-mesenchymal transition (EMT) is an essential physiologic process that promotes cancer cell migration, invasion, and metastasis. Several lines of evidence from both cellular genetic studies suggest AKT1/PKBα, but not AKT2 or AKT3, serves as a negative regulator EMT breast However, the underlying mechanism by which AKT1 suppresses remains poorly defined. Here, we demonstrate phosphorylation Twist1 required for β-TrCP–mediated ubiquitination degradation. The clinically...
The sensitivity of non-small cell lung cancer (NSCLC) patients to EGFR tyrosine kinase inhibitors (TKIs) is strongly associated with activating mutations. Although not as sensitive harboring these mutations, some wild-type (wtEGFR) remain responsive TKIs, suggesting that the existence unexplored mechanisms renders most wtEGFR-expressing cells insensitive.Here, we show acquired resistance an TKI, gefitinib, elevated expression breast protein (BCRP/ABCG2), which in turn leads gefitinib efflux...
Abstract Derailed transmembrane receptor trafficking could be a hallmark of tumorigenesis and increased tumor invasiveness, but dynamics have not been used to differentiate metastatic cancer cells from less invasive ones. Using single-particle tracking techniques, we developed phenotyping asssay named T ransmembrane Re ceptor D ynamics (TReD), studied the epidermal growth factor (EGFR) in seven breast epithelial cell lines assay (TReD). Here show clear evidence that EGFR diffusivity enlarged...