A5059502052- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Receptor Mechanisms and Signaling
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Pharmacological Effects and Assays
- Metal-Catalyzed Oxygenation Mechanisms
- Liver Disease Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Asthma and respiratory diseases
- Viral Infections and Vectors
- Herpesvirus Infections and Treatments
- Diet, Metabolism, and Disease
- Diet and metabolism studies
- Animal Disease Management and Epidemiology
- Influenza Virus Research Studies
- Cancer-related molecular mechanisms research
- Plant Virus Research Studies
- Virology and Viral Diseases
- SARS-CoV-2 and COVID-19 Research
- RNA modifications and cancer
- Cassava research and cyanide
- Metabolomics and Mass Spectrometry Studies
- MicroRNA in disease regulation
- Mass Spectrometry Techniques and Applications
Southwest Medical University
2022-2025
Zhejiang University
2008-2024
Shenyang Pharmaceutical University
2018-2024
China Ocean Shipping (China)
2023
Pfizer (United States)
2015-2022
Ministry of Agriculture and Rural Affairs
2018-2022
TEDA International Cardiovascular Hospital
2021
Tianjin Geothermal Exploration, Development and Design Institute (China)
2019
Nanjing Agricultural University
2015-2018
Pennsylvania State University
2004-2016
In a conventional class I ribonucleotide reductase (RNR), diiron(II/II) cofactor in the R2 subunit reacts with oxygen to produce diiron(III/IV) intermediate, which generates stable tyrosyl radical (Y⚫). The Y⚫ reversibly oxidizes cysteine residue R1 cysteinyl (C⚫), abstracts 3′-hydrogen of substrate initiate its reduction. RNR from Chlamydia trachomatis lacks Y⚫, and it had been proposed that complex directly C⚫ R1. By enzyme activity measurements spectroscopic methods, we show this actually...
Pseudorabies virus (PRV) causes Aujeszky's disease in pigs and can be transmitted to other mammals, including humans. In the current study, we systematically studied interspecies transmission evolutionary history of PRV.We performed comprehensive analysis on phylodynamics, selection, structural biology summarize phylogenetic adaptive evolution PRV based all available full-length major glycoprotein sequences.PRV divided into 2 main clades with frequent interclade intraclade recombination....
Abstract Porcine circovirus 3 (PCV3) is a novel virus associated with acute PDNS (porcine dermatitis and nephropathy syndrome)‐like clinical signs identified by metagenomic sequencing from swine. Its high occurrence may pose potential threat to the swine industry worldwide. The processes resulting in emergence spread of PCV3 remain poorly understood. Herein, possible origin, genotypes, evolutionary dynamics based on available genomic sequences are determined. closest ancestor found be within...
The enzyme isopenicillin N synthase (IPNS) installs the β-lactam and thiazolidine rings of penicillin core into linear tripeptide l-δ-aminoadipoyl-l-Cys-d-Val (ACV) on pathways to a number important antibacterial drugs. A classic set enzymological crystallographic studies by Baldwin co-workers established that this overall four-electron oxidation occurs sequence two oxidative cyclizations, with ring being installed first second. Each phase requires cleavage an aliphatic C–H bond substrate:...
Abstract The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has shown once again that (CoV) in animals are potential sources for epidemics humans. Porcine deltacoronavirus (PDCoV) is an emerging enteropathogen swine with a worldwide distribution. Here, we implemented and described approach to analyze the epidemiology PDCoV following its pig population. We performed integrated analysis full genome sequence data from 21 newly sequenced viruses, along comprehensive...
To investigate the evolutionary process by which porcine epidemic diarrhea virus (PEDV) in United States hypothetically descended from strains China, we analyzed PEDV-positive samples collected China during January 2012-July 2013. Recombination 2 strain sublineages was likely associated with identification of PEDV
Recent studies suggest that excess dietary fructose contributes to metabolic dysfunction by promoting insulin resistance, de novo lipogenesis (DNL), and hepatic steatosis, thereby increasing the risk of obesity, type 2 diabetes (T2D), non-alcoholic steatohepatitis (NASH), related comorbidities. Whether this is driven calories contained in or whether catabolism itself uniquely pathogenic remains controversial. We sought test a small molecule inhibitor primary metabolizing enzyme...
myo -Inositol oxygenase (MIOX) activates O 2 at a mixed-valent nonheme diiron(II/III) cluster to effect oxidation of its cyclohexan-(1,2,3,4,5,6-hexa)-ol substrate [ -inositol (MI)] by four electrons d -glucuronate. Abstraction hydrogen from C 1 formally (superoxo)diiron(III/III) intermediate was previously proposed. Use deuterium-labeled substrate, 1,2,3,4,5,6-[ H] 6 -MI (D -MI), has now permitted initial characterization the C–H-cleaving intermediate. The MIOX·1,2,3,4,5,6-[ complex reacts...
Acetylation by ARD1 of an inhibitor mammalian target rapamycin may inhibit cancer growth.
Increased fructose consumption and its subsequent metabolism have been implicated in metabolic disorders such as nonalcoholic fatty liver disease steatohepatitis (NAFLD/NASH) insulin resistance. Ketohexokinase (KHK) converts to fructose-1-phosphate (F1P) the first step of cascade. Herein we report discovery a first-in-class KHK inhibitor, PF-06835919 (8), currently phase 2 clinical trials. The 8 was built upon our originally reported, fragment-derived lead 1 recognition an alternative,...
Porcine circovirus 3 (PCV3) was found to be associated with reproductive disease in pigs, and since its first identification the United States, it subsequently spread worldwide, especially China, where might pose a potential threat porcine industry. However, no exhaustive analysis performed understand evolution prospect of codon usage pattern. Here, we deep PCV3. PCV3 sequences were classified into two clades: PCV3a PCV3b, confirmed by principal component analysis. Additionally, degree bias...
Myeloperoxidase (MPO) is a heme peroxidase that catalyzes the production of hypochlorous acid. Clinical evidence suggests causal role for MPO in various autoimmune and inflammatory disorders including vasculitis cardiovascular Parkinson's diseases, implying inhibitors may represent therapeutic treatment option. Herein, we present design, synthesis, preclinical evaluation N1-substituted-6-arylthiouracils as potent selective MPO. Inhibition proceeded time-dependent manner by covalent,...
Branched chain amino acid (BCAA) catabolic defects are implicated to be causal determinates of multiple diseases. This work aimed better understand how enhancing BCAA catabolism affected metabolic homeostasis as well the mechanisms underlying these improvements. The rate limiting step is irreversible decarboxylation by branched ketoacid dehydrogenase (BCKDH) enzyme complex, which post-translationally controlled through phosphorylation BCKDH kinase (BDK). study utilized BT2, a small molecule...
False positives that arise when MS/MS data are used to search protein sequence databases remain a concern in proteomics research. Here, we present five types of false identified aligning sequences spectra by Mascot database searching software. because (1) enzymatic digestion at abnormal sites; (2) misinterpretation charge states; (3) modifications; (4) incorrect assignment the modification site; and (5) use isotopic peaks. We examples, clearly as manual inspection, nevertheless were assigned...
Increased fructose consumption and its subsequent metabolism have been implicated in hepatic steatosis, dyslipidemia, obesity, insulin resistance humans. Since ketohexokinase (KHK) is the principal enzyme responsible for metabolism, identification of a selective KHK inhibitor may help to further elucidate effect inhibition on these metabolic disorders. Until now, studies with small molecules limited due lack viable vivo pharmacological tools. Herein we report discovery 12, potency properties...
Exploring the anti-tumor molecular mechanisms of traditional Chinese medicines has become an important strategy to develop novel drugs in clinic. Several pharmacological studies have reported antioxidant, antibacterial, anti-inflammatory, and effects clove. Previously, we shown that active fraction from clove (AFC) can inhibit growth tumor cells, particularly colon cancer vitro . However, mechanism action regarding anti-colon activity AFC, especially aerobic glycolysis, not been adequately...