A5031307446- Aquaculture disease management and microbiota
- Invertebrate Immune Response Mechanisms
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Immune Response and Inflammation
- interferon and immune responses
- Aquaculture Nutrition and Growth
- Animal Disease Management and Epidemiology
- T-cell and B-cell Immunology
- Viral gastroenteritis research and epidemiology
- Environmental Toxicology and Ecotoxicology
- Physiological and biochemical adaptations
- Mosquito-borne diseases and control
- Influenza Virus Research Studies
- Herpesvirus Infections and Treatments
- CRISPR and Genetic Engineering
- Immunotherapy and Immune Responses
- Viral Infections and Vectors
- Inflammasome and immune disorders
- Virology and Viral Diseases
- Sirtuins and Resveratrol in Medicine
- Glutathione Transferases and Polymorphisms
- Nuclear Receptors and Signaling
- Immune Cell Function and Interaction
Zhejiang University
2014-2024
Ministry of Agriculture and Rural Affairs
2022
The large yellow croaker Larimichthys crocea (L. crocea) is one of the most economically important marine fish in China and East Asian countries. It also exhibits peculiar behavioral physiological characteristics, especially sensitive to various environmental stresses, such as hypoxia air exposure. These traits may render L. a good model for investigating response mechanisms stress. To understand molecular genetic underlying adaptation stress, we sequenced assembled genome using bacterial...
Abstract Background Systematic research on fish immunogenetics is indispensable in understanding the origin and evolution of immune systems. This has long been a challenging task because limited number deep sequencing technologies genome backgrounds non-model available. The newly developed Solexa/Illumina RNA-seq Digital gene expression (DGE) are high-throughput approaches powerful tools for genomic studies at transcriptome level. study reports profiling analysis bacteria-challenged...
The long-held paradigm that B cells cannot uptake nonspecific particulate Ags for the initiation of primary adaptive immunity has been challenged by recent discovery teleost have potent phagocytic and microbicidal abilities. This provides preliminary clues primitive might act as initiating APCs in priming immunity. In this study, zebrafish clearly showed a Ag-presenting ability to both soluble bacterial particles prime naive CD4(+) T cell activation. finding demonstrates innate-like nature...
Ubiquitination is an important reversible post-translational modification. Many viruses hijack the host ubiquitin system to enhance self-replication. In present study, we found that Avibirnavirus VP3 protein was ubiquitinated during infection and supported virus replication by ubiquitination. Mass spectrometry mutation analysis showed at residues K73, K135, K158, K193, K219. Virus rescue ubiquitination sites K219 on could abilities of infectious bursal disease (IBDV), K135 essential for...
Abstract Dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN/CD209) has become hot topic in recent studies because of its important roles immune responses and escape. CD209 been well characterized humans several other mammals, but little documentation exists about it lower vertebrates. This is the first report on identification functional characterization a fish DC-SIGN/CD209 molecule. The zebrafish cDNA translates into 343 aa organized three domains structurally conserved among An...
NLRP1 inflammasome is one of the best-characterized inflammasomes in humans and other mammals. However, existence this nonmammalian species remains poorly understood. In study, we report molecular functional identification an homolog, Danio rerio (DrNLRP1) from a zebrafish (D. rerio) model. This DrNLRP1 possesses similar structural architecture to mammalian NLRP1s. It can trigger formation classical for activation inflammatory caspases Caspase [DrCaspase]-A DrCaspase-B) maturation D. IL-1β...
Tripartite motif-containing protein 21 (TRIM21), an E3 ubiquitin ligase, plays a critical role in the host antiviral response. However, mechanism and spectrum of TRIM21 influenza A virus (IAV) remain unclear. Here, we report that inhibits replication various IAV subtypes by targeting matrix 1 (M1) from H3/H5/H9, but not H1 H7 M1. Mechanistically, binds to residue R95 M1 facilitates K48 ubiquitination K242 for proteasome-dependent degradation, leading inhibition H3, H5, H9 replication....
Viruses have developed different strategies to hijack mitophagy facilitate their replication. However, whether and how African swine fever virus (ASFV) regulates are largely unknown. Here, we found that the ASFV-encoded p17 induced mitophagy. Coimmunoprecipitation/mass spectrometry assays identified translocase of outer mitochondrial membrane 70 (TOMM70) as protein interacted with p17. The binding TOMM70 promoted receptor SQSTM1 TOMM70, led engulfment mitochondria by autophagosomes,...
Abstract Inflammatory bowel disease (IBD) has long been a worldwide health care problem with persistently increasing incidence. Although its clinical features have well described, etiology and pathogenesis remain unclear. IL-16 is chemoattractant cytokine various effects on cellular activities diseases. However, the involvement of in IBD remains poorly understood. In this study, to our knowledge we report for first time mechanism by which induces intestinal inflammation upregulating...
Peroxiredoxin (Prx) was previously known as a Cys-dependent thioredoxin. However, we unexpectedly observed that Prx1 from the green spotted puffer fish Tetraodon nigroviridis (TnPrx1) able to reduce H2O2 in manner independent of Cys peroxidation and reductants. This study aimed validate novel function for Prx1, delineate biochemical features explore its antioxidant role cells. We have confirmed humans truly possesses catalase (CAT)-like activity is residues reductants, but dependent on iron....
The use of antibiotic resistance genes in plasmids causes potential biosafety and clinical hazards, such as the possibility horizontal spread or rapid emergence multidrug-resistant pathogens. This paper introduces a novel auxotrophy complementation system that allowed host cells to be effectively selected maintained without antibiotics. An Escherichia coli strain carrying defect NAD de novo biosynthesis was constructed by knocking out chromosomal quinolinic acid phosphoribosyltransferase...
Abstract Toll–IL-1R (TIR) family members play crucial roles in a variety of defense, inflammatory, injury, and stress responses. Although they have been widely investigated mammals, little is known about TIRs ancient vertebrates. In this study, we report novel double Ig IL-1R related molecule (DIGIRR) from three model fish (Tetraodon nigroviridis, Gasterosteus aculeatus, Takifugu rubripes), adding previously unknown homolog to the TIR family. This DIGIRR contains two Ig-like domains...
In an effort to reconstitute the NAD+ synthetic pathway in Escherichia coli (E. coli), we produced a set of gene knockout mutants with deficiencies previously well-defined de novo and salvage pathways. Unexpectedly, mutant deficient I could grow M9/nicotinamide medium, which was contradictory proposed classic metabolism E. coli. Such mutagenesis assay suggested presence undefined machinery feed nicotinamide into biosynthesis. We wanted verify whether xanthosine phophorylase (xapA)...
Abstract The establishment of high sensitive detection method for various pathogenic microorganisms remains constantly concerned. In the present study, multi-probe strategy was first systematically investigated followed by establishing a highly TaqMan real-time fluorescent quantitative PCR (qPCR) detecting African swine fever virus (ASFV). Briefly, four probes based on B646L gene ASFV were designed and effects different combinations in single qPCR assay sensitivity investigated. As less as...
Entry of circovirus into the host cell nucleus is essential for viral replication during early stage infection. However, mechanisms by which nucleolar shuttle proteins are used still not well understood. Here, we report a previously unidentified localization signal in capsid protein (Cap), and that hijacks phosphoprotein nucleophosmin-1 (NPM1) to facilitate its replication. Colocalization analysis showed NPM1 translocates from nucleolus nucleoplasm cytoplasm Coimmunoprecipitation glutathione...
Abstract The transport of circovirus capsid protein into nucleus is essential for viral replication in infected cell. However, the role nucleolar shuttle proteins during porcine 3 (PCV3 Cap) import still not understood. Here, we report a previously unidentified localization signal (NoLS) PCV3 Cap, which hijacks phosphoprotein nucleophosmin-1 (NPM1) to facilitate Cap. NoLS Cap and serine-48 residue N-terminal oligomerization domain NPM1 are Cap/NPM1 interaction. In addition, charge property...
Calreticulin (CRT), a well conserved endoplasmic reticulum-resident protein for Ca2+-binding, is widely expressed in multicellular eukaryotes. CRT plays key role many cellular processes, including Ca2+ homeostasis. To address the of underlying homeostasis alternation during mineralization cycle mud crab (Scylla paramamosain), we cloned full-length cDNA gene (SpCRT) from S. paramamosain, and its contains all signature domains CRT. Tissue distribution showed SpCRT transcript was far more...
Virus-host interaction is a tug of war between pathogenesis and immunity, followed by either activating the host immune defense system to eliminate virus or manipulating control mechanisms survive facilitate propagation. Comprehensive knowledge interactions viral proteins might provide hints for developing novel antiviral strategies. To gain more detailed with porcine circovirus type 2 capsid protein, we employed coimmunoprecipitation combined liquid chromatography mass spectrometry approach...