A5027937699- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA Research and Splicing
- Circular RNAs in diseases
- Cancer Immunotherapy and Biomarkers
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Cancer Mechanisms and Therapy
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- Cancer Genomics and Diagnostics
- Immune cells in cancer
- Chemokine receptors and signaling
- Ubiquitin and proteasome pathways
- Cancer Research and Treatments
- Cancer Cells and Metastasis
- Hepatocellular Carcinoma Treatment and Prognosis
- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
- Cancer, Stress, Anesthesia, and Immune Response
- Bone Tissue Engineering Materials
- Cancer-related gene regulation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- interferon and immune responses
Fudan University Shanghai Cancer Center
2016-2025
Shanghai Medical College of Fudan University
2013-2025
Central South University
2016-2025
Xiangya Hospital Central South University
2022-2025
Fudan University
2012-2025
Wenzhou Medical University
2025
University of South China
2025
Shanghai Jiao Tong University
2011-2024
Nanjing University
2024
Henan University Huaihe Hospital and Huaihe Clinical Institute
2023-2024
Abstract Circular RNAs (circRNAs) represent a class of widespread and diverse endogenous that may regulate gene expression in eukaryotes. However, the regulation function human circRNAs remain largely unknown. Here we generate ribosomal-depleted RNA sequencing data from six normal tissues seven cancers, detect at least 27,000 circRNA candidates. Many these are differently expressed between cancerous tissues. We further characterize one abundant derived Exon2 HIPK3 gene, termed circHIPK3. The...
Exosomes, which are nanosized endocytic vesicles that secreted by most cells, contain an abundant cargo of different RNA species can modulate the behavior recipient cells and may be used as circulating biomarkers for diseases. Here, we develop a web-accessible database (http://www.exoRBase.org), exoRBase, is repository circular (circRNA), long non-coding (lncRNA) messenger (mRNA) derived from RNA-seq data analyses human blood exosomes. Experimental validations published literature also...
Apoptosis is critical for embryonic development, tissue homeostasis, and tumorigenesis determined largely by the Bcl-2 family of antiapoptotic prosurvival regulators. Here, we report that glycogen synthase kinase 3 (GSK-3) was required Mcl-1 degradation, identified a novel mechanism proteasome-mediated turnover in which GSK-3beta associates with phosphorylates at one consensus motif ((155)STDG(159)SLPS(163)T; phosphorylation sites are italics), will lead to association E3 ligase beta-TrCP,...
Abstract MicroRNAs (miRNAs) are important gene regulators, which often deregulated in cancers. In this study, the authors analyzed microRNAs profiles of 78 matched cancer/noncanerous liver tissues from HCC patients and 10 normal found that 69 miRNAs were differentially expressed between hepatocellular carcinoma (HCC) corresponding noncancerous (N). Then expressions 8 validated by real time RT PCR. The set could distinctly classify HCC, N (NL). Moreover, some these related to clinical factors...
Abstract Purpose: MicroRNAs (miRNA) have been documented playing a critical role in cancer development and progression. In this study, we investigate the of miR-148a gastric metastasis. Experimental Design: We examined levels 90 samples by qRT-PCR analyzed clinicopathologic significance expression. The cells stably expressing miRNA-148a were for migration invasion assays vitro metastasis vivo; target genes further explored. Results: found that expression was suppressed more than 4-fold...
Extracellular vesicles (EVs) contain a rich cargo of different RNA species with specialized functions and clinical applications. However, the landscape characteristics extracellular vesicle long (exLR) in human blood remain largely unknown.We presented an optimized strategy for exLR sequencing (exLR-seq) plasma. The sample cohort included 159 healthy individuals, 150 patients cancer (5 types), 43 other diseases. Bioinformatics approaches were used to analyze distribution features exLRs....
Cancer cells possess a unique metabolic phenotype that allows them to preferentially utilize glucose through aerobic glycolysis. This phenomenon is referred as the “Warburg effect.” Accumulating evidence suggests microRNAs (miRNAs), class of small noncoding regulatory RNAs, interact with oncogenes/tumor suppressors and induce such reprograming in cancer cells. To systematically study roles miRNAs cells, we developed gain‐of‐function miRNA screen HeLa Subsequent investigation characterized...
Long noncoding RNAs can serve as oncogenes or tumor suppressors in human cancer; however, their biological functions and underlying mechanism hepatocarcinogenesis are largely unknown. Here, we report a novel suppressor long RNA on chromosome 8p12 (termed TSLNC8 ) that is frequently deleted down‐regulated hepatocellular carcinoma (HCC) tissues. The loss of highly associated with the malignant features HCC serves prognostic indicator for patients. significantly suppresses proliferation...
Triple-negative breast cancer (TNBC) is a highly heterogeneous group of cancers, and molecular subtyping necessary to better identify molecular-based therapies. While some classifiers have been established, no one has integrated the expression profiles long noncoding RNAs (lncRNAs) into such criterions. Considering emerging important role lncRNAs in cellular processes, novel classification integrating transcriptome both messenger RNA (mRNA) lncRNA would help us understand heterogeneity TNBC....
Recurrent chromosomal aberrations have led to the discovery of oncogenes or tumour suppressors involved in carcinogenesis. Here we characterized an oncogenic long intergenic non-coding RNA frequent DNA-gain regions hepatocellular carcinoma (HCC), LINC01138 (long located on 1q21.2). The locus is frequently amplified HCC; transcript stabilized by insulin like growth factor-2 mRNA-binding proteins 1/3 (IGF2BP1/IGF2BP3) and associated with malignant features poor outcomes HCC patients. acts as...
Objective Pancreatic ductal adenocarcinoma (PDAC) is difficult to diagnose at resectable stage. Recent studies have suggested that extracellular vesicles (EVs) contain long RNAs. The aim of this study was develop a diagnostic (d-)signature for the detection PDAC based on EV RNA (exLR) profiling. Design We conducted case-control with 501 participants, including 284 patients PDAC, 100 chronic pancreatitis (CP) and 117 healthy subjects. exLR profile plasma samples analysed by sequencing....
Long noncoding RNAs (lncRNAs) have been associated with hepatocellular carcinoma (HCC), but the underlying molecular mechanisms of their specific association hepatocarcinogenesis not fully explored. Methods: miR503HG was identified by microarray and validated real-time PCR. Survival analysis evaluated using Kaplan-Meier method assessed log-rank test. In vitro in vivo assays were preformed to explore biological effects HCC cells. The interaction HNRNPA2B1 RNA pull-down immunoprecipitation....
Human cancer cell lines are fundamental models for research and therapeutic strategy development. However, there is no characterization of circular RNAs (circRNAs) in a large number lines.Here, we apply four circRNA identification algorithms to heuristically characterize the expression landscape circRNAs across ~ 1000 human from CCLE polyA-enriched RNA-seq data. By using integrative analysis experimental approaches, explore landscape, biogenesis, functional consequences, drug response...
MicroRNAs (miRNAs) are small, noncoding RNAs that can act as oncogenes or tumor suppressors in human cancer. Our previous study showed miR-125b was a prognostic indicator for patients with hepatocellular carcinoma (HCC), but its functions and exact mechanisms hepatic carcinogenesis still unknown. Here we demonstrate suppressed HCC cell growth vitro vivo . Moreover, increased p21Cip1/Waf1 expression arrested cycle at G1 to S transition. In addition, inhibited migration invasion. Further...
The pathological relevance and significance of microRNAs (miRNAs) in hepatocarcinogenesis have attracted much attention recent years; however, little is known about the underlying molecular mechanisms through which miRNAs are involved development progression hepatocellular carcinoma (HCC). In this study, we demonstrate that miR-30d frequently up-regulated HCC its expression highly associated with intrahepatic metastasis HCC. Furthermore, enhanced could promote cell migration invasion vitro...
Transforming growth factor-beta (TGF-beta) plays a dual and complex role in human cancer. In this report, we observe specific set of MicroRNAs (miRNAs) changed response to TGF-beta hepatocellular carcinoma (HCC) cells by miRNA microarray screening. A cluster miRNA, miR-23a approximately 27a 24, is induced an early stage Huh-7 cells. Knockdown Smad4, Smad2 or Smad3 expression RNA interference can attenuate the 24 addition, indicating that induction dependent on Smad pathway. We also explore...
Myeloid cell leukemia-1 (Mcl-1), an antiapoptotic Bcl-2 family member, is overexpressed in many types of human cancer and associates with immortalization, malignant transformation, chemoresistance. Glycogen synthase kinase-3beta (GSK-3beta), a key component the Wnt signaling pathway, involved multiple physiologic processes such as protein synthesis, tumorigenesis, apoptosis. Here, we report that expression Mcl-1 was correlated phosphorylated GSK-3beta (p-GSK-3beta) at Ser(9) (an inactivated...
Abstract As the “master” microRNA that is induced by hypoxia, miR-210 involved in multiple processes hypoxia pathway. However, whether mediates hypoxia-induced tumor cell metastasis still remains unclear. Here, we demonstrate frequently up-regulated hepatocellular carcinoma (HCC) samples and promotes migration invasion of HCC cells. Furthermore, can be cells metastasis. We identify vacuole membrane protein 1 (VMP1) as direct functional downstream target miR-210; addition, show its expression...