A5100324049- Epigenetics and DNA Methylation
- RNA modifications and cancer
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- Autophagy in Disease and Therapy
- Cancer Cells and Metastasis
- Circular RNAs in diseases
- RNA Research and Splicing
- Liver Disease Diagnosis and Treatment
- Advanced Proteomics Techniques and Applications
- Immunotherapy and Immune Responses
- Prostate Cancer Treatment and Research
- DNA Repair Mechanisms
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Chromatography
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Peptidase Inhibition and Analysis
- Histone Deacetylase Inhibitors Research
Shanghai Jiao Tong University
2016-2025
Tianjin Medical University
2016-2025
Harbin Medical University
2021-2025
Nankai University
2010-2025
Songjiang District Central Hospital
2025
Fudan University
2012-2025
Collaborative Innovation Center of Chemical Science and Engineering Tianjin
2025
Beijing Shijitan Hospital
2024-2025
Capital Medical University
2020-2025
Sun Yat-sen University
2011-2025
Previous studies on the pneumonia outbreak caused by 2019 novel coronavirus disease (COVID-19) were mainly based information from adult populations. Limited data are available for children with COVID-19, especially infected infants.We report a 55-day-old case COVID-19 confirmed in China and describe identification, diagnosis, clinical course, treatment of patient, including progression day 7 to 11 illness.This highlights that can also present multiple organ damage rapid changes.When managing...
The conversion of male germ cell chromatin to a nucleoprotamine structure is fundamental the life cycle, yet underlying molecular details remain obscure. Here we show that an essential step genome-wide incorporation TH2B, histone H2B variant hitherto unknown function. Using mouse models in which TH2B depleted or C-terminally modified, directs final transformation dissociating nucleosomes into protamine-packed structures. Depletion induces compensatory mechanisms permit removal by...
The histone demethylase PHF8 has been implicated in multiple pathological disorders, including X-linked mental retardation and tumorigenesis. However, it is not clear how the abundance function of are regulated. Here, we report that physically associates with deubiquitinase USP7. Specifically, demonstrated USP7 promotes deubiquitination stabilization PHF8, leading to upregulation a group genes, cyclin A2, critical for cell growth proliferation. USP7-encoding gene was also transcriptionally...
Abstract Programmed cell death protein-1 (PD-1)/programmed ligand-1 (PD-L1) interaction plays a crucial role in tumor-associated immune escape. Here, we verify that triple-negative breast cancer (TNBC) has higher PD-L1 expression than other subtypes. We then discover nucleophosmin (NPM1) binds to promoter specifically TNBC cells and activates transcription, thus inhibiting T activity vitro vivo. Furthermore, demonstrate PARP1 suppresses transcription through its with the nucleic acid binding...
Cell plasticity and neuroendocrine differentiation in prostate lung adenocarcinomas are one of the major reasons for therapeutic resistance to targeted therapy. Whether how metabolic changes contribute this adenocarcinoma-to-neuroendocrine cell fate transition remains largely unclear. Here we show that or cancer cells possess mostly fragmented mitochondria with low membrane potential rely on glycolysis energy metabolism. We further an important role determinant Numb mitochondrial quality...
Lysine lactylation (Kla) links metabolism and gene regulation plays a key role in multiple biological processes. However, the regulatory mechanism functional consequence of Kla remain to be explored. Here, we report that HBO1 functions as lysine lactyltransferase regulate transcription. We show catalyzes addition vitro intracellularly, E508 is site for activity HBO1. Quantitative proteomic analysis further reveals 95 endogenous sites targeted by HBO1, with majority located on histones. Using...
Protein lysine acetylation plays a key role in regulating chromatin dynamics, gene expression and metabolic pathways eukaryotes, and, thus, contributes to diverse cellular processes like transcription, cell cycle regulation, apoptosis. Although recent evidence suggests that acetylated proteins impact broadly functions prokaryotes, the substrates localization of this modification remain widely unknown due limitations analytical methods. Comprehensive identification protein is major bottleneck...
A GC-MS method was developed for the determination of various flavonoids and phenolic benzoic acids in human plasma. The procedure involved extraction with ethyl acetate, followed by derivatization compounds BSTFA (N,O-bis(trimethylsilyl) trifluoroacetamide) + TMCS (trimethylchlorosilane) reagent. trimethylsilyl derivatives formed were separated quantitated using GC-MS. Twenty have been well spiked plasma without any interference. average recovery 79.3%. Several such as o-hydroxybenzoic,...
Genome-wide association study (GWAS) is nowadays widely used to identify genes involved in human complex disease. The standard GWAS analysis examines SNPs/genes independently and identifies only a number of the most significant SNPs. It ignores combined effect weaker SNPs/genes, which leads difficulties explore biological function mechanism from systems point view. Although gene set enrichment (GSEA) has been introduced overcome these limitations by identifying correlation between...
Abstract Cancer-testis ( CT ) genes represent the similarity between processes of spermatogenesis and tumorigenesis. It is possible that their selective expression pattern can help identify driver in cancer. In this study, we integrate transcriptomics data from multiple databases systematically 876 new 19 cancer types. We explore relationship with testis-specific regulatory elements. propose extremely highly expressed (EECTGs) are potential drivers activated through epigenetic mechanisms....
Abstract Proto-oncogene Akt plays essential roles in cell proliferation and tumorigenesis. Full activation of is regulated by phosphorylation, ubiquitination, acetylation. Here we report that SUMOylation a novel mechanism for its activation. Systematically analyzing the role lysine residues revealed K276, which located consensus motif, Ectopic or endogenous Akt1 could be modified SUMOylation. RNA interference–mediated silencing UBC9 reduced SUMOylation, was promoted SUMO E3 ligase PIAS1...
Significance The ability of printing single-cell arrays with high precision and efficiency, resolution, multiple cell types, maintenance viability function is essential for heterogeneity measurement. It still hard current methods to completely satisfy the above requirements. We report a unique live-cell technique, Block-Cell-Printing, that allows convenient, precise, multiplexed, high-throughput functional arrays. Block-Cell-Printing has minimum turnaround time 0.5 h, maximum resolution 5...
Abstract IFNγ is conventionally recognized as an inflammatory cytokine that plays a central role in antitumor immunity. Although it has been used clinically to treat variety of malignancies, low levels the tumor microenvironment (TME) increase risk metastasis during immunotherapy. Accumulating evidence suggests can induce cancer progression, yet mechanisms underlying controversial development remain unclear. Here, we reveal dose-dependent effect inducing stemness accelerate progression...
Angiogenesis is a complex process regulating endothelial cell (EC) functions. Emerging lines of evidence support that YAP (Yes-associated protein) plays an important role in the angiogenic activity ECs.The objective this study was to specify effect EC on angiogenesis and its underlying mechanisms.In ECs, vascular growth factor reduced phosphorylation time dose dependently increased nuclear accumulation. Using Tie2Cre-mediated transgenic mice, we found promoted postnatal retina tumor tissues....
Rationale: Angiogenesis promotes neurological recovery after stroke and is associated with longer survival of patients. Cerebral angiogenesis tightly controlled by certain microRNAs (miRs), such as the miR-15a/16-1 cluster, among others. However, function cluster in endothelium on postischemic cerebral not known. Objective: To investigate functional significance molecular mechanism endothelial ischemic brain. Methods Results: Endothelial cell-selective conditional knockout (EC-miR-15a/16-1...