A5042785102- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Estrogen and related hormone effects
- Receptor Mechanisms and Signaling
- Histone Deacetylase Inhibitors Research
- Protein Kinase Regulation and GTPase Signaling
- Metabolism, Diabetes, and Cancer
- Coccidia and coccidiosis research
- Pluripotent Stem Cells Research
- Protein Degradation and Inhibitors
- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Viral Infectious Diseases and Gene Expression in Insects
- Ubiquitin and proteasome pathways
- Sperm and Testicular Function
- Kruppel-like factors research
- Cancer-related gene regulation
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Chromosomal and Genetic Variations
- Platelet Disorders and Treatments
- Pancreatic function and diabetes
- RNA Research and Splicing
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
Inserm
2012-2022
Université Grenoble Alpes
2011-2022
Centre National de la Recherche Scientifique
2016-2022
Institut pour l'avancée des biosciences
2011-2022
Institut de Biosciences et Biotechnologies
2017-2018
Université Joseph Fourier
2010-2017
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
1994-2010
CEA Grenoble
1994-2010
Institut de Recherches en Technologies et Sciences pour le Vivant
2010
Centre de Recherche Saint-Antoine
1993
Recently discovered histone lysine acylation marks increase the functional diversity of nucleosomes well beyond acetylation. Here, we focus on butyrylation in context sperm cell differentiation. Specifically, investigate H4 5 and 8 at gene promoters where acetylation guides binding Brdt, a bromodomain-containing protein, thereby mediating stage-specific expression programs post-meiotic chromatin reorganization. Genome-wide mapping data show that highly active Brdt-bound systematically harbor...
The conversion of male germ cell chromatin to a nucleoprotamine structure is fundamental the life cycle, yet underlying molecular details remain obscure. Here we show that an essential step genome-wide incorporation TH2B, histone H2B variant hitherto unknown function. Using mouse models in which TH2B depleted or C-terminally modified, directs final transformation dissociating nucleosomes into protamine-packed structures. Depletion induces compensatory mechanisms permit removal by...
Protein kinase CK2 is a ubiquitous protein implicated in proliferation and cell survival. Its regulatory beta subunit, CK2beta, which encoded by single gene mammals, has been suspected of regulating other kinases. In this work, we show that knockout the CK2beta mice leads to postimplantation lethality. Mutant embryos were reduced size at embryonic day 6.5 (E6.5). They did not exhibit signs apoptosis but proliferation. resorbed E7.5. vitro, CK2beta(-/-) morula development stopped after...
Vascular endothelial–cadherin (VE–cadherin) is exclusively expressed in endothelial cells and strictly located at cell-to-cell junctions. As the other members of cadherin family, VE–cadherin able to mediate a homotypic type cellular interaction Ca 2+ -dependent manner. In mouse embryo, transcripts are detected earliest stages vascular development. To ascertain if expression required for assembly into structures, we generated -negative embryonic stem ( −/− ES cells) by gene targeting examined...
Nuclear protein in testis (Nut) is a universal oncogenic driver the highly aggressive NUT midline carcinoma, whose physiological function male germ cells has been unclear. Here we show that expression of Nut normally restricted to post-meiotic spermatogenic cells, where its presence triggers p300-dependent genome-wide histone H4 hyperacetylation, which essential for completion histone-to-protamine exchange. Accordingly, inactivation induces sterility with spermatogenesis arrest at...
The chicken lysozyme gene can be induced in oviduct cells by four classes of steroid hormones, including glucocorticosteroids and progestins. glucocorticosteroid receptor rat liver the progesterone rabbit uterus both bind, although with different relative affinities, to two sites promoter region located, respectively, between 50 80 160 200 base pairs upstream transcription start point. Now we show that purified binding unit (Mr 110,000, or so-called B subunit) generates a DNase I protection...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOligomeric structure of molybdate-stabilized, nontransformed 8S progesterone receptor from chicken oviduct cytosolJack Michel Renoir, Thierry Buchou, Jan Mester, Christine Radanyi, and Etienne Emile BaulieuCite this: Biochemistry 1984, 23, 25, 6016–6023Publication Date (Print):December 1, 1984Publication History Published online1 May 2002Published inissue 1 December 1984https://doi.org/10.1021/bi00320a018Request reuse permissionsArticle...
A 110kDa component of the chick oviduct progesterone receptor (PR) has been purified to homogeneity according electrophoretic criteria and specific activity (assuming one progestagen-binding site/110kDa). The procedure involved affinity chromatography 0.3 M-KCl-prepared cytosol, followed by DEAE-Sephacel (elution at 0.2 M-KCl). final yield was about 12% in terms binding activity. Properties indicate that it is identical with ‘B’ subunit described previously [Stokes radius approximately 6.1...
Genetic programs that govern neural stem/progenitor cell (NSC) proliferation and differentiation are dependent on extracellular cues a network of transcription factors, which can be regulated posttranslationally by phosphorylation. However, little is known about the kinase-dependent pathways regulating NSC maintenance oligodendrocyte development. We used conditional knockout approach to target murine regulatory subunit (beta) protein kinase casein 2 (CK2beta) in embryonic progenitors. Loss...
Protamines confer a compact structure to the genome of male gametes. Here, we find that somatic cells can be remodeled by transient expression protamine 1 (Prm1). Ectopically expressed Prm1 forms scattered foci in nuclei fibroblasts, which coalescence into spermatid-like structures, concomitant with loss histones and reprogramming barrier, H3 lysine 9 methylation. Protaminized injected enucleated oocytes efficiently underwent maternal histone TH2B exchange developed normal blastocyst stage...
Altering the biochemical makeup of chromatin by incorporation histone variants during development represents a key mechanism in regulating gene expression. The variant H2A.B, H2A.B.3 mice, appeared late evolution and is most highly expressed testis. In mouse, it encoded three different genes. H2A.B expression spatially temporally regulated spermatogenesis being haploid round spermatid stage. Active genes gain where directly interacts with polymerase II RNA processing factors within splicing...
The release of Agrin by motoneurons activates the muscle-specific receptor tyrosine kinase (MuSK) as main organizer subsynaptic specializations at neuromuscular junction. MuSK downstream signaling is largely undefined. Here we show that protein CK2 interacts and colocalizes with post-synaptic specializations. We observed CK2-mediated phosphorylation serine residues within insert (KI) MuSK. Inhibition or knockdown CK2, exchange phosphorylatable serines alanines KI MuSK, impaired acetylcholine...
Nontransformed 8S progesterone receptor (8S-PR) was purified by hormone-specific affinity chromatography from rabbit uterine low-salt cytosol containing 20 mM molybdate. In the eluate obtained with radioactive progestin, sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) showed presence of several bands, including three that corresponded to approximately 90-, 120-, and 85-kDa proteins. None these proteins found in column when molybdate-containing chromatographed nonradioactive...
Effects of salt, heat, and ATP on the rate sedimentation chick oviduct progesterone receptor (PR) were examined under various conditions. Cytosol [3H]PR complex (PRc) sedimented as an 8S molecule in 10-35% glycerol or 5- 20% sucrose gradients. Incubation cytosol containing [3H]PRc at either 23 0 C with 0.3 M KC1 5-10 mM for 1-4 h resulted appearance a slower migrating form approximately 4S complete concomitant disappearance PR form. This transformation was inhibited by molybdate paralleled...