A5088366849- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Circadian rhythm and melatonin
- Histone Deacetylase Inhibitors Research
- CRISPR and Genetic Engineering
- HIV Research and Treatment
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Adipose Tissue and Metabolism
- Hedgehog Signaling Pathway Studies
- Liver Disease Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- Sleep and Wakefulness Research
- Diet, Metabolism, and Disease
- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- Cancer Genomics and Diagnostics
- Sperm and Testicular Function
- Light effects on plants
- Stress Responses and Cortisol
- DNA Repair Mechanisms
- Cancer-related molecular mechanisms research
- Tryptophan and brain disorders
- Dietary Effects on Health
- Molecular Biology Techniques and Applications
Centre National de la Recherche Scientifique
2016-2025
Inserm
2013-2025
Université Grenoble Alpes
2012-2025
University of California, Irvine
2017-2023
Institut pour l'avancée des biosciences
2009-2022
Institut de Biosciences et Biotechnologies
2017
Université Joseph Fourier
2009-2014
Recently discovered histone lysine acylation marks increase the functional diversity of nucleosomes well beyond acetylation. Here, we focus on butyrylation in context sperm cell differentiation. Specifically, investigate H4 5 and 8 at gene promoters where acetylation guides binding Brdt, a bromodomain-containing protein, thereby mediating stage-specific expression programs post-meiotic chromatin reorganization. Genome-wide mapping data show that highly active Brdt-bound systematically harbor...
The conversion of male germ cell chromatin to a nucleoprotamine structure is fundamental the life cycle, yet underlying molecular details remain obscure. Here we show that an essential step genome-wide incorporation TH2B, histone H2B variant hitherto unknown function. Using mouse models in which TH2B depleted or C-terminally modified, directs final transformation dissociating nucleosomes into protamine-packed structures. Depletion induces compensatory mechanisms permit removal by...
Post-translational modifications of proteins have emerged as a major mechanism for regulating gene expression. However, our understanding how histone directly affect chromatin function remains limited. In this study, we investigate acetylation H3 at lysine 64 (H3K64ac), previously uncharacterized on the lateral surface octamer. We show that H3K64ac regulates nucleosome stability and facilitates eviction hence expression in vivo. line with this, demonstrate is enriched vivo transcriptional...
Aging is accompanied by impairments in both circadian rhythmicity and long-term memory. Although it clear that memory performance affected cycling, unknown whether age-related disruption of the clock causes impaired hippocampal Here, we show repressive histone deacetylase HDAC3 restricts memory, synaptic plasticity, experience-induced expression gene Per1 aging hippocampus without affecting rhythmic activity patterns. We also demonstrate critical for formation. Together, our data challenge...
Obstructive sleep apnea (OSA) induces intermittent hypoxia (IH), an independent risk factor for non-alcoholic fatty liver disease (NAFLD). While the molecular links between IH and NAFLD progression are unclear, immune cell-driven inflammation plays a crucial role in pathogenesis. Using lean mice exposed to long-term cohort of OSA patients (n = 71), we conducted comprehensive hepatic transcriptomics, lipidomics, targeted serum proteomics. Significantly, demonstrated that alone can induce NASH...
Binge drinking and chronic exposure to ethanol contribute alcoholic liver diseases (ALDs). A potential link between ALDs circadian disruption has been observed, though how different patterns of alcohol consumption differentially impact hepatic metabolism remains virtually unexplored. Using acute versus feeding, we reveal differential reprogramming the transcriptome in liver. Specifically, rewiring diurnal SREBP transcriptional pathway leads distinct signatures acetyl-CoA that are translated...
Abstract Li-Fraumeni syndrome is a cancer predisposition caused by pathogenic TP53 germline variants and associated with high lifelong risk. We analysed the German LFS registry that contains data on 304 individuals. Cancer phenotypes were correlated grouped according to their ability transactivate target genes in yeast assay using traditional (non-functional, partially-functional) novel (clusters A, B, C) classification of into different groups. Partially-functional cluster B or C enriched...
The molecular basis of post-meiotic male genome reorganization and compaction constitutes one the last black boxes in modern biology. Although successive transitions DNA packaging have been well described, factors driving these near genome-wide reorganizations remain obscure. We used a combination different approaches aiming at discovery critical capable directing reprogramming, which is now shedding new light on nature fundamental mechanisms controlling histone replacement compaction. Here...
Abstract Stem cells, poised to revolutionize current medicine, stand as major workhorses for monitoring changes in cell fate. Characterizing metabolic phenotypes is key monitor differentiating cells transcriptional and epigenetic shifts at a functional level provides non-genetic means control specification. Expanding the arsenal of analytical tools profiling differentiation therefore importance. Here, we describe metabolome whole pluripotent stem (PSCs) using high‐resolution magic angle...
Sleep Apnea Syndrome (SAS) is one of the most common chronic diseases, affecting nearly billion people worldwide. The repetitive occurrence abnormal respiratory events generates cyclical desaturation-reoxygenation sequences known as intermittent hypoxia (IH). Among SAS metabolic sequelae, it has been established by experimental and clinical studies that an independent risk factor for development progression non-alcoholic fatty liver disease (NAFLD). principal goal this study was to decrypt...
Abstract Metabolic syndrome has increased at a worrisome level. Lifestyle changes are not sufficient to prevent and improve the adverse effects of obesity, thus novel interventions necessary. The aim this study was investigate use metabolic outcomes non-pharmacological intervention in high-fat diet (HFD) fed mouse model, capable recapitulating key aspects syndrome. We show that Policaptil Gel Retard remarkable, beneficial on dysfunction caused by consumption HFD. describe mechanism which...
ABSTRACT Li-Fraumeni syndrome (LFS) has recently been redefined as a ‘spectrum’ cancer predisposition disorder to reflect its broad phenotypic heterogeneity. The wide functional gradient associated with different TP53 variants is thought contribute LFS heterogeneity, although it still poorly understood and there an unmet clinical need for risk stratification strategies. Leveraging p53 mutagenesis dataset, we performed unsupervised cluster analysis that revealed five variant clusters unique...
Abstract Advancements in genotyping and sequencing techniques, coupled with the growing magnitude of genome databases, have led to comprehensive investigations into impact genetic variants on human diseases, including cancers. It is commonly acknowledged that homogeneous effects clinical outcomes, i.e., exhibit similar within a certain group but are distinct from those other groups. However, such grouping information typically unknown, identification underlying variation groups crucial for...