A5018260367- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- BRCA gene mutations in cancer
- Breast Cancer Treatment Studies
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Breast Implant and Reconstruction
- Cancer-related Molecular Pathways
- Health and Medical Studies
- Genetic factors in colorectal cancer
- Ovarian cancer diagnosis and treatment
- Mesenchymal stem cell research
- Social and Demographic Issues in Germany
- Breast Lesions and Carcinomas
- Cancer-related molecular mechanisms research
- Diversity and Career in Medicine
- Circular RNAs in diseases
- DNA Repair Mechanisms
- Musculoskeletal Disorders and Rehabilitation
- Endometrial and Cervical Cancer Treatments
- Cancer therapeutics and mechanisms
- Immunotherapy and Immune Responses
- Cancer Treatment and Pharmacology
- Radiopharmaceutical Chemistry and Applications
- Molecular Biology Techniques and Applications
Heidelberg University
2016-2025
University Hospital Heidelberg
2015-2024
Boise State University
2021
Frauenklinik Heidelberg
2011-2020
Klinik für Frauenheilkunde
2014-2020
University Hospital Schleswig-Holstein
2020
University of Lübeck
2020
National Center for Tumor Diseases
2010-2019
German Cancer Research Center
2012-2019
Wellcome Centre for Integrative Neuroimaging
2019
In recent years, circulating miRNAs have attracted a great deal of attention as promising novel markers for various diseases. Here, we investigated their potential to serve minimally invasive, early detection breast cancer in blood plasma. We profiled extracted from the plasma stage patients (taken at time-point diagnosis) and healthy control individuals using TaqMan low-density arrays (TLDA). Selected candidates identified initial screen were further validated an extended study cohort 207...
Lymphoid and myeloid cells are abundant in the tumor microenvironment, can be quantified by immunohistochemistry shape disease course of human solid tumors. Yet, there is no comprehensive understanding spatial immune infiltration patterns ('topography') across cancer entities various cell types. In this study, we systematically measure topography multiple types 965 histological tissue slides from N = 177 patients a pan-cancer cohort. We provide definition inflamed ('hot'), non-inflamed...
The use of circulating tumor cells (CTC) as a prognostic marker in metastatic breast cancer (MBC) has been well established. However, their efficacy and accuracy are still under scrutiny mainly because methods enrichment identification. We hypothesized that miRNAs can predict the CTC status patients with MBC, tested for same. Furthermore, we aimed at establishing panel capable differentiating MBC cases from healthy controls.Circulating plasma CTC-positive CTC-negative controls, were profiled...
<h3>Purpose</h3> To characterise the prevalence of pathogenic germline mutations in <i>BRCA1</i> and <i>BRCA2</i> families with breast cancer (BC) ovarian (OC) history. <h3>Patients methods</h3> Data from 21 401 were gathered between 1996 2014 a clinical setting German Consortium for Hereditary Breast Ovarian Cancer, comprising full pedigrees status all individual members at time first counselling, <i>BRCA1/2</i> mutation index patient. <h3>Results</h3> The overall was 24.0% (95% CI 23.4% to...
Metastasis is the principal cause of high morbidity and mortality among breast cancer (BC) patients. Identification markers that can be routinely monitored to predict onset metastasis in BC patients prognosis metastatic (MBC) would increase their median survival. In this study, plasma miRNAs 40 MBC were profiled by TaqMan low density arrays with prognostic capacity identified. The candidates validated initially samples 237 subsequently 335 from an independent study cohort Sixteen established...
Over the last few years, circulating microRNAs (miRNAs) have emerged as promising novel and minimally invasive markers for various diseases, including cancer. We already showed that certain miRNAs are deregulated in plasma of breast cancer patients when compared to healthy women. Herein we further explored their potential serve early detection blood plasma. Circulating miR-127-3p, miR-376a miR-652, selected candidates from a miRNA array-based screening, were found be associated with first...
Abstract The immune system can recognize and attack cancer cells, especially those with a high load of mutation-induced neoantigens. Such neoantigens are abundant in DNA mismatch repair (MMR)-deficient, microsatellite-unstable (MSI) cancers. MMR deficiency leads to insertion/deletion (indel) mutations at coding microsatellites (cMS) neoantigen-inducing translational frameshifts. Here, we develop tool quantify frameshift MSI colorectal endometrial cancer. Our results show that mutation...
Abstract The 19p13.1 breast cancer susceptibility locus is a modifier of risk in BRCA1 mutation carriers and also associated with the ovarian cancer. Here, we investigated variation subtypes, defined by estrogen receptor (ER), progesterone (PR), human epidermal growth factor receptor-2 (HER2) status, using 48,869 cases 49,787 controls from Breast Cancer Association Consortium (BCAC). Variants were not overall or ER-positive but significantly ER-negative [rs8170 OR, 1.10; 95% confidence...
To evaluate virtual touch tissue imaging quantification (VTIQ) as a new elastography method concerning its intra- and interexaminer reliability ability to differentiate benign from malignant breast lesions in comparison combination with ultrasound (US) B-mode reporting data system (BI-RADS) assessment. US VTIQ were performed by two examiners 103 women 104 lesions. Intra- of was assessed. The area under the receiver operating curve (AUC), sensitivity, specificity, positive predictive value...
To prospectively assess circulating tumor cell (CTC) status at baseline (CTCBL) and after one cycle of a new line systemic therapy (CTC1C), changes from CTCBL to CTC1C (CTC kinetics, CTCKIN) for their utility in predicting response, progression-free (PFS) overall survival (OS) metastatic breast cancer (MBC). was determined as negative (-) or positive (+) < 5 ≥ CTCs/7.5 ml blood using CellSearch™ (Veridex). CTCKIN categorized favorable (CTC1C-) unfavorable (CTC1C+). Tumor response be assessed...
In the last decade, circulating nucleic acids such as microRNAs (miRNAs) and cell-free DNA (cfDNA) have become increasingly important in serving potential novel biomarkers for a variety of human diseases. If are to routinely used diagnostics, difference plasma miRNA cfDNA levels between healthy diseased subjects must exceed pre-analytical analytical variability. Until now, few studies addressed time limitations pre-processing or explored use long-term blood storage tubes, which might need be...
Abstract Background Ovarian cancer (OC) is an aggressive disease, primarily diagnosed in late stages with only 20% of patients surviving more than 5 years after diagnosis. There a pending need to improve current diagnostics and prognostics. Methods In this study, we investigated total circulating cell-free microRNA (cf-miRNA) levels as well panel cf-miRNAs the plasma OC ( n = 100), benign lesions 45) healthy controls 99). Results High correlated unfavourable clinical features were...
Abstract Li-Fraumeni syndrome is a cancer predisposition caused by pathogenic TP53 germline variants and associated with high lifelong risk. We analysed the German LFS registry that contains data on 304 individuals. Cancer phenotypes were correlated grouped according to their ability transactivate target genes in yeast assay using traditional (non-functional, partially-functional) novel (clusters A, B, C) classification of into different groups. Partially-functional cluster B or C enriched...
Fibronectin is ubiquitously expressed in the extracellular matrix, and experimental evidence has shown that it modulates blood vessel formation. The relative contribution of local circulating fibronectin to formation vivo remains unknown despite for unexpected roles various diseases. Using transgenic mouse models, we established facilitates growth bone metastases by enhancing maturation. This effect more relevant than produced endothelial cells pericytes, which only exert a small additive on...
Introduction Vaginal intraepithelial neoplasia (VAIN) is a pre-malignant lesion, potentially leading to vaginal cancer. It rare disease, representing less than 1% of all the female genital tract. Similar cervical (CIN), there are three different grades VAIN. VAIN 1 also known as low-grade squamous lesion (LSIL), whereas 2 and 3 both represent high-grade lesions (HSIL). Risk factors for development similar those neoplasia, i.e. promiscuity, starting sexual activity at an early age, tobacco...
Breast cancer (BC) is the leading cause of cancer‐related mortality in women worldwide. Changes DNA methylation peripheral blood could be associated with malignancy at early stage. However, BC‐associated signatures were largely unknown. Here, we performed a genome‐wide screening and identified differentially methylated CpG site cg27091787 hyaluronoglucosaminidase 2 gene ( HYAL2 ) (discovery round 72 BC case 24 controls: p = 2.61 × 10 −9 adjusted for cell‐type proportions). The substantially...
In recent years, metabolites have attracted substantial attention as promising novel biomarkers of various diseases. However, breast cancer plasma metabolite studies are still in their infancy. Here, we investigated the potential to serve minimally invasive, early detection markers primary cancer. We profiled extracted from patients and healthy controls using tandem mass spectrometry (UHPLC‐MS/MS FIA‐MS/MS). Two were found be upregulated, while 16 downregulated compared both training...