A5062338945- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- MicroRNA in disease regulation
- Histone Deacetylase Inhibitors Research
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- vaccines and immunoinformatics approaches
- Chromatin Remodeling and Cancer
- Molecular Biology Techniques and Applications
- Systemic Lupus Erythematosus Research
- CAR-T cell therapy research
- Advanced biosensing and bioanalysis techniques
- Prostate Cancer Treatment and Research
- Prostate Cancer Diagnosis and Treatment
- Plant Toxicity and Pharmacological Properties
- Phagocytosis and Immune Regulation
- T-cell and B-cell Immunology
- HIV Research and Treatment
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- Click Chemistry and Applications
Affimed Therapeutics (Germany)
2024
Heidelberg University
2014-2023
University Hospital Heidelberg
2019-2023
Epigenomics (Germany)
2010-2017
German Cancer Research Center
2014-2017
DKFZ-ZMBH Alliance
2017
Abstract The comparison of DNA methylation patterns across cancer types (pan-cancer methylome analyses) has revealed distinct subgroups tumors that share similar patterns. Integration these data with the wealth information derived from genome profiling studies performed by large international consortia provided novel insights into cellular aberrations contribute to development. There is evidence genetic mutations in epigenetic regulators (such as DNMT3, IDH1/2 or H3.3) mediate patterns,...
Abstract Juvenile myelomonocytic leukemia (JMML) is an aggressive myeloproliferative disorder of early childhood characterized by mutations activating RAS signaling. Established clinical and genetic markers fail to fully recapitulate the biological heterogeneity this disease. Here we report DNA methylome analysis mutation profiling 167 JMML samples. We identify three subgroups with unique molecular characteristics. The high methylation group (HM) somatic PTPN11 poor outcome. low enriched for...
In the last decade, circulating nucleic acids such as microRNAs (miRNAs) and cell-free DNA (cfDNA) have become increasingly important in serving potential novel biomarkers for a variety of human diseases. If are to routinely used diagnostics, difference plasma miRNA cfDNA levels between healthy diseased subjects must exceed pre-analytical analytical variability. Until now, few studies addressed time limitations pre-processing or explored use long-term blood storage tubes, which might need be...
Abstract Background Ovarian cancer (OC) is an aggressive disease, primarily diagnosed in late stages with only 20% of patients surviving more than 5 years after diagnosis. There a pending need to improve current diagnostics and prognostics. Methods In this study, we investigated total circulating cell-free microRNA (cf-miRNA) levels as well panel cf-miRNAs the plasma OC ( n = 100), benign lesions 45) healthy controls 99). Results High correlated unfavourable clinical features were...
Aberrant DNA methylation at specific genetic loci is a key molecular feature of juvenile myelomonocytic leukemia (JMML) with poor prognosis. Using quantitative high-resolution mass spectrometry, we identified RASA4 isoform 2, which maps to chromosome 7 and encodes member the GAP1 family GTPase-activating proteins for small G proteins, as recurrent target isoform-specific hypermethylation in JMML (51% 125 patients analyzed). 2 promoter correlated clinical parameters predicting prognosis...
Abstract Ovarian cancer (OC) is an aggressive disease, primarily diagnosed in late stages with only 20% of patients surviving more than 5 years. Liquid biopsy markers have great potential to improve current diagnostic and prognostic methods. Here, we compared miRNAs DNA methylation matched plasma, whole blood tissues as a surrogate marker for OC. We found that while both cfDNA cf-miRNAs levels were upregulated OC benign lesions or healthy controls, cf-miRNA independent prognosticator...
Abstract Background Aberrant DNA methylation is frequently found in human malignancies including acute myeloid leukemia (AML). While most studies focus on later disease stages, the onset of aberrant events and their dynamics during leukemic progression are largely unknown. Methods We screened genome-wide for CpG island three stages a murine AML model that driven by hypomorphic expression hematopoietic transcription factor PU.1. levels selected genes were correlated with CD34+ cells lineage...
A-kinase anchor protein 12 (AKAP12) is a regulator of kinase A and C signaling, acting downstream RAS. Epigenetic silencing AKAP12 has been demonstrated in different cancer entities this linked to the process tumorigenesis. Here, we used quantitative high-resolution DNA methylation measurement by MassARRAY investigate epigenetic regulation all three promoters (i.e., α, β, γ) within large cohort juvenile myelomonocytic leukemia (JMML) patient samples. The AKAP12α promoter shows...
Juvenile myelomonocytic leukemia (JMML) is a myeloproliferative neoplasm of childhood whose clinical heterogeneity only poorly represented by gene sequence alterations. It was previously shown that aberrant DNA methylation distinct target genes defines more aggressive variant JMML, but few significant targets are known so far. To get broader picture disturbed CpG patterns in we carried out screen 34 candidate 45 patients using quantitative mass spectrometry. Five analyzed showed recurrent...
Abstract Background Although breast cancer (BC) has a high survival rate, relapse events may occur which ultimately lead to aggressive disease. Circulating cell-free microRNAs (cf-miRNAs) are promising minimally invasive biomarker with diagnostic and/or prognostic potential. Unfortunately, there is still no consensus as universal cf-miRNA in BC and been clinical implementation until now. One major limitation the technical variation isolation specific quantification methods. Methods In this...
Abstract Purpose Endometrial carcinoma is the second most common gynecological malignancy. Until today lacking a screening tool. A blood-based biomarker could help address this need. Methods The expression levels of 30 acylcarnitines, 18 amino acids, 6 miRNAs, and 7 DNA methylation sites were measured in blood samples from 331 women (20 EC, 14 benign uterine lesions (benign), 140 breast cancers (BC), 157 controls). Areas under ROC curves (AUC), sensitivity (sens.) specificity (spec.)...
The aim of this study was to determine the allele frequencies genetic variants CCR5delta32, CCR2-64I, and SDF1-3'A (SDF1 801 A), which influence susceptibility HIV-1 infection. We also investigated effect these on general Ecuadoran population a group HIV-infected individuals frequency genetics variants.