A5077473496- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Gene expression and cancer classification
- Chronic Lymphocytic Leukemia Research
- Cancer Cells and Metastasis
- Cancer-related molecular mechanisms research
- Bioinformatics and Genomic Networks
- RNA modifications and cancer
- Acute Myeloid Leukemia Research
- Statistical Methods and Inference
- Cancer Genomics and Diagnostics
- Pregnancy and preeclampsia studies
- RNA Interference and Gene Delivery
- Computational Drug Discovery Methods
- Genetic Associations and Epidemiology
- Genetic factors in colorectal cancer
- Birth, Development, and Health
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Glioma Diagnosis and Treatment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Immunotherapy and Biomarkers
- Cancer-related gene regulation
- Chronic Myeloid Leukemia Treatments
- Statistical Methods in Clinical Trials
- Bayesian Methods and Mixture Models
University of Oslo
2016-2025
Oslo University Hospital
2020-2024
German Cancer Research Center
2010-2023
Heidelberg University
2010-2023
Epigenomics (Germany)
2012-2023
University of Cambridge
2022
Mucolipidosis IV Foundation
2019
DKFZ-ZMBH Alliance
2010-2016
Cancer Research And Biostatistics
2014
The Ohio State University
2012-2013
Purpose To analyze the frequency and prognostic impact of isocitrate dehydrogenase 1 (IDH1) 2 (IDH2) mutations in acute myeloid leukemia (AML). Patients Methods We studied 805 adults (age range, 16 to 60 years) with AML enrolled on German-Austrian Study Group (AMLSG) treatment trials HD98A APL HD95 for exon 4 IDH1 IDH2. were also NPM1, FLT3, MLL, CEBPA mutations. The median follow-up survival was 6.3 years. Results IDH found 129 patients (16.0%) —IDH1 61 (7.6%), IDH2 70 (8.7%). Two had both...
In recent years, circulating miRNAs have attracted a great deal of attention as promising novel markers for various diseases. Here, we investigated their potential to serve minimally invasive, early detection breast cancer in blood plasma. We profiled extracted from the plasma stage patients (taken at time-point diagnosis) and healthy control individuals using TaqMan low-density arrays (TLDA). Selected candidates identified initial screen were further validated an extended study cohort 207...
Biliary tract cancers (BTC) are relatively rare malignant tumours with poor prognosis. It is known from other solid neoplasms that antitumour inflammatory response has an impact on tumour behaviour and patient outcome. The aim of this study was to provide a comprehensive characterisation in human BTC. Tumour-infiltrating T lymphocytes (CD4+, CD8+, Foxp3+), natural killer cells (perforin+), B (CD20+), macrophages (CD68+) as well mast (CD117+) were assessed by immunohistochemistry 375 BTC...
Despite much evidence on epigenetic abnormalities in cancer, it is currently unclear to what extent alterations can be associated with tumors' clonal genetic origins. Here, we show that the prostate intratumor heterogeneity DNA methylation and copy-number patterns explained by a unified evolutionary process. By assaying multiple topographically distinct tumor sites, premalignant lesions, lymph node metastases within five cases of demonstrate both consistently reflect life history tumors....
The use of circulating tumor cells (CTC) as a prognostic marker in metastatic breast cancer (MBC) has been well established. However, their efficacy and accuracy are still under scrutiny mainly because methods enrichment identification. We hypothesized that miRNAs can predict the CTC status patients with MBC, tested for same. Furthermore, we aimed at establishing panel capable differentiating MBC cases from healthy controls.Circulating plasma CTC-positive CTC-negative controls, were profiled...
We assessed the prognostic impact of IDH1 R132 mutations and a known single nucleotide polymorphism (SNP) located in same exon gene patients with cytogenetically normal acute myeloid leukemia (CN-AML) context other markers.IDH1 four was directly sequenced 275 CN-AML from two subsequent AML multicenter treatment trials 120 healthy volunteers. Moreover, NPM1, FLT3, CEBPA, WT1 were analyzed, mRNA expression quantified.IDH1 found 10.9% patients. SNP rs11554137 12% 11.7% had no on prognosis. In...
Non-coding RNAs are much more common than previously thought. However, for the vast majority of non-coding RNAs, cellular function remains enigmatic. The two long RNA (lncRNA) genes DLEU1 and DLEU2 map to a critical region at chromosomal band 13q14.3 that is recurrently deleted in solid tumors hematopoietic malignancies like chronic lymphocytic leukemia (CLL). While no point mutations have been found protein coding candidate 13q14.3, they deregulated malignant cells, suggesting an epigenetic...
Although clonal selection by genetic driver aberrations in cancer is well documented, the ability of epigenetic alterations to promote tumor evolution undefined. We used 450k arrays and next-generation sequencing evaluate intratumor heterogeneity DNA methylation chronic lymphocytic leukemia (CLL). CLL cases exhibit vast interpatient differences heterogeneity, with genetically maintaining low up 10% total CpGs a monoallelically methylated state. Increasing correlates advanced subclonal...
Metastasis is the principal cause of high morbidity and mortality among breast cancer (BC) patients. Identification markers that can be routinely monitored to predict onset metastasis in BC patients prognosis metastatic (MBC) would increase their median survival. In this study, plasma miRNAs 40 MBC were profiled by TaqMan low density arrays with prognostic capacity identified. The candidates validated initially samples 237 subsequently 335 from an independent study cohort Sixteen established...
Over the last few years, circulating microRNAs (miRNAs) have emerged as promising novel and minimally invasive markers for various diseases, including cancer. We already showed that certain miRNAs are deregulated in plasma of breast cancer patients when compared to healthy women. Herein we further explored their potential serve early detection blood plasma. Circulating miR-127-3p, miR-376a miR-652, selected candidates from a miRNA array-based screening, were found be associated with first...
Dysregulated microRNA (miRNA) expression contributes to the pathogenesis of hematopoietic malignancies, including chronic lymphocytic leukemia (CLL). However, an understanding mechanisms that cause aberrant miRNA transcriptional control is lacking. In this study, we comprehensively investigated role and extent epigenetic regulation in CLL. Genome-wide profiling conducted on 24 CLL 10 healthy B cell samples revealed global DNA methylation patterns upstream sequences distinguished malignant...
Increased ZAP-70 expression predicts poor prognosis in chronic lymphocytic leukemia (CLL). Current methods for accurately measuring are problematic, preventing widespread application of these tests clinical decision making. We therefore used comprehensive DNA methylation profiling the regulatory region to identify sites important transcriptional control.High-resolution quantitative analysis entire gene regions was conducted on 247 samples from patients with CLL four independent...
The molecular mechanisms underlying the genesis of cholangiocarcinomas (CCs) are poorly understood. Epigenetic changes such as aberrant hypermethylation and subsequent atypical gene expression characteristic features most human cancers. In CC, data regarding global methylation lacking so far. We performed a genome-wide analysis for promoter in CCs. profiled 10 intrahepatic 8 extrahepatic CCs comparison to non-neoplastic biliary tissue specimens, using methyl-CpG immunoprecipitation (MCIp)...
Abstract Purpose: To evaluate the effect of thymidylate synthase (TYMS) and methylenetetrahydrofolate reductase (MTHFR) genotypes on toxicity in patients treated with capecitabine for advanced colorectal cancer to determine these polymorphisms pretreatment levels serum folate plasma homocysteine. Experimental Design: Fifty-four a diagnosis metastatic were fixed-dose capecitabine. Germ line DNA from was genotyped TYMS TSER, TSER*3G>C, 3′-untranslated 6 bp insertion/deletion (3′...
Antibody microarrays have the potential to enable comprehensive proteomic analysis of small amounts sample material. Here, protocols are presented for production, quality assessment, and reproducible application antibody in a two-color mode with an array 1,800 features, representing 810 antibodies that were directed at 741 cancer-related proteins. In addition measures quality, we implemented indicators accuracy significance dual-color detection. Dual-color measurements outperform...
Several studies have documented the variety of post-stroke psychosocial challenges, which are complex, multifaceted, and affect a patient's rehabilitation recovery. Due to consequences these well-being should be considered an important outcome stroke rehabilitation. Thus, valid reliable instrument that is appropriate for population required. The factor structure Norwegian version GHQ-28 has not previously been examined when applied population. purpose this study was explore psychometric...
Abstract Motivation Identification of genomic, molecular and clinical markers prognostic patient survival is important for developing personalized disease prevention, diagnostic treatment approaches. Modern omics technologies have made it possible to investigate the impact at multiple levels, including genomics, epigenomics, transcriptomics, proteomics metabolomics, how these potential risk factors complement characterization outcomes prognosis. However, massive sizes datasets, along with...