A5026607584- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- Colorectal Cancer Treatments and Studies
- Lung Cancer Diagnosis and Treatment
- Head and Neck Cancer Studies
- Genetic factors in colorectal cancer
- Cancer Immunotherapy and Biomarkers
- Pancreatic and Hepatic Oncology Research
- Cancer-related molecular mechanisms research
- Lung Cancer Research Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Chronic Lymphocytic Leukemia Research
- Prostate Cancer Treatment and Research
- Radiomics and Machine Learning in Medical Imaging
- PARP inhibition in cancer therapy
- RNA modifications and cancer
- Prostate Cancer Diagnosis and Treatment
- Cancer Diagnosis and Treatment
- Neuroendocrine Tumor Research Advances
- Epigenetics and DNA Methylation
- BRCA gene mutations in cancer
- Sarcoma Diagnosis and Treatment
- Chromatin Remodeling and Cancer
- Cancer Mechanisms and Therapy
- Advanced Breast Cancer Therapies
University Hospital Heidelberg
2016-2025
Heidelberg University
2016-2025
Deutschen Konsortium für Translationale Krebsforschung
2016-2025
German Center for Lung Research
2018-2025
German Cancer Research Center
2016-2025
National Center for Tumor Diseases
2014-2024
Chirurgische Universitätsklinik Heidelberg
2019-2024
Heidelberg University
2012-2023
Metropolitan University
2023
Bar-Ilan University
2022
Purpose Our aim was to analyze and validate the prognostic impact of novel International Association for Study Lung Cancer (IASLC)/American Thoracic Society (ATS)/European Respiratory (ERS) proposal an architectural classification invasive pulmonary adenocarcinomas (ADCs) across all tumor stages. Patients Methods The pattern a large cohort 500 patients with resected ADCs (stages I IV) retrospectively analyzed in 5% increments classified according their predominant architecture (lepidic,...
Tumor mutational burden (TMB), defined as the number of somatic mutations per megabase interrogated genomic sequence, varies across malignancies. Panel sequencing-based estimates TMB have largely replaced whole-exome sequencing-derived in clinic. Retrospective evidence suggests that can predict efficacy immune checkpoint inhibitors, and data from KEYNOTE-158 led to recent FDA approval pembrolizumab for TMB-high tumor subgroup. Unmet needs include prospective validation cutoffs relationship...
Infection-related diabetes can arise as a result of virus-associated β-cell destruction. Clinical data suggest that the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causing disease 2019 (COVID-19), impairs glucose homoeostasis, but experimental evidence SARS-CoV-2 infect pancreatic tissue has been lacking. In present study, we show infects cells human exocrine and endocrine pancreas ex vivo in vivo. We demonstrate β-cells express viral entry proteins, replicates cultured...
Background Tumor mutational burden (TMB), defined as the number of somatic mutations per megabase interrogated genomic sequence, demonstrates predictive biomarker potential for identification patients with cancer most likely to respond immune checkpoint inhibitors. TMB is optimally calculated by whole exome sequencing (WES), but next-generation targeted panels provide estimates in a time-effective and cost-effective manner. However, differences panel size gene coverage, addition underlying...
Abstract Sarcomas are malignant soft tissue and bone tumours affecting adults, adolescents children. They represent a morphologically heterogeneous class of some entities lack defining histopathological features. Therefore, the diagnosis sarcomas is burdened with high inter-observer variability misclassification rate. Here, we demonstrate classification using machine learning classifier algorithm based on array-generated DNA methylation data. This sarcoma trained dataset 1077 profiles from...
The identification of gene fusions from RNA sequencing data is a routine task in cancer research and precision oncology. However, despite the availability many computational tools, fusion detection remains challenging. Existing methods suffer poor prediction accuracy are computationally demanding. We developed Arriba, novel algorithm with high sensitivity short runtime. When applied to large collection published pancreatic samples ( n = 803), Arriba identified variety driver fusions, which...
// Jan Budczies 1,2,6,* , Moritz von Winterfeld 1,* Frederick Klauschen 1 Michael Bockmayr Jochen K. Lennerz 3 Carsten Denkert 1,6 Thomas Wolf 4,6 Arne Warth 4 Manfred Dietel Ioannis Anagnostopoulos Wilko Weichert 4,6,7 Daniel Wittschieber 5 and Albrecht Stenzinger Institute of Pathology, Charité University Hospital, Berlin, Germany 2 German Cancer Research Center (DKFZ), Heidelberg, Massachusetts General Hospital/Harvard Medical School, Department Boston, MA, USA Hospital Legal...
Background Men suspected of having clinically significant prostate cancer (sPC) increasingly undergo MRI. The potential deep learning to provide diagnostic support for human interpretation requires further evaluation. Purpose To compare the performance clinical assessment a system optimized segmentation trained with T2-weighted and diffusion MRI in task detection lesions suspicious sPC. Materials Methods In this retrospective study, sequences from consecutive men examined single 3.0-T...
Leiomyosarcoma (LMS) is an aggressive mesenchymal malignancy with few therapeutic options. The mechanisms underlying LMS development, including clinically actionable genetic vulnerabilities, are largely unknown. Here we show, using whole-exome and transcriptome sequencing, that tumors characterized by substantial mutational heterogeneity, near-universal inactivation of TP53 RB1, widespread DNA copy number alterations chromothripsis, frequent whole-genome duplication. Furthermore, detect...
Biliary tract cancers (BTC) are relatively rare malignant tumours with poor prognosis. It is known from other solid neoplasms that antitumour inflammatory response has an impact on tumour behaviour and patient outcome. The aim of this study was to provide a comprehensive characterisation in human BTC. Tumour-infiltrating T lymphocytes (CD4+, CD8+, Foxp3+), natural killer cells (perforin+), B (CD20+), macrophages (CD68+) as well mast (CD117+) were assessed by immunohistochemistry 375 BTC...
Automated image analysis of cells and tissues has been an active research field in medical informatics for decades but recently attracted increased attention due to developments computer microscopy hardware the awareness that scientific diagnostic pathology require novel approaches perform objective quantitative analyses cellular tissue specimens. Model-based use a priori information on cell shape features obtain segmentation, which may introduce bias favouring detection nuclei only with...
Abstract We used whole-genome and transcriptome sequencing to identify clinically actionable genomic alterations in young adults with pancreatic ductal adenocarcinoma (PDAC). Molecular characterization of 17 patients PDAC enrolled a precision oncology program revealed gene fusions amenable pharmacologic inhibition by small-molecule tyrosine kinase inhibitors all KRAS wild-type (KRASWT) tumors (4 17). These included recurrent NRG1 rearrangements predicted drive development through aberrant...
Method16 January 2020Open Access Source DataTransparent process Automated sample preparation with SP3 for low-input clinical proteomics Torsten Müller German Cancer Research Center (DKFZ), Heidelberg, Germany Medical Faculty, Heidelberg University, Search more papers by this author Mathias Kalxdorf EMBL, Rémi Longuespée Department of Clinical Pharmacology and Pharmacoepidemiology, Daniel N Kazdal orcid.org/0000-0001-8187-3281 Institute Pathology, Albrecht Stenzinger Jeroen Krijgsveld...
Abstract The clinical relevance of comprehensive molecular analysis in rare cancers is not established. We analyzed the profiles and outcomes 1,310 patients (rare cancers, 75.5%) enrolled a prospective observational study by German Cancer Consortium that applies whole-genome/exome RNA sequencing to inform care adults with incurable cancers. On basis 472 single six composite biomarkers, cross-institutional tumor board provided evidence-based management recommendations, including diagnostic...
Lynch syndrome is caused by germline mutations of DNA mismatch repair (MMR) genes. MMR deficiency has long been regarded as a secondary event in the pathogenesis colorectal cancers. Recently, this concept challenged discovery MMR‐deficient crypt foci normal mucosa. We aimed to reconstruct carcinogenesis collecting molecular and histology evidence from adenomas carcinomas. determined frequency mutation carriers immunohistochemistry systematic literature analysis. To trace back pathways...