A5079694973- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Cancer Research and Treatments
- Cancer Immunotherapy and Biomarkers
- MicroRNA in disease regulation
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Extracellular vesicles in disease
- Cancer-related Molecular Pathways
- Colorectal Cancer Treatments and Studies
- Immune cells in cancer
- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Glioma Diagnosis and Treatment
- NF-κB Signaling Pathways
- Epigenetics and DNA Methylation
- HER2/EGFR in Cancer Research
- Single-cell and spatial transcriptomics
- Immunotherapy and Immune Responses
- Estrogen and related hormone effects
- Prostate Cancer Diagnosis and Treatment
- Mathematical Biology Tumor Growth
- Cancer-related molecular mechanisms research
- Cellular Mechanics and Interactions
- Phagocytosis and Immune Regulation
German Cancer Research Center
2014-2025
DKFZ-ZMBH Alliance
2014-2025
Heidelberg University
2014-2025
Heidelberg Institute for Stem Cell Technology and Experimental Medicine
2013-2025
Helmholtz Zentrum München
2020-2023
ETH Zurich
2021-2023
Fondazione IRCCS Istituto Neurologico Carlo Besta
2022-2023
University Hospital of Basel
2019-2020
University of Basel
2019
Digital Research Alliance of Canada
2018
Abstract Circulating tumor cells (CTCs) drive metastasis, the leading cause of death in individuals with breast cancer. Due to their low abundance circulation, robust CTC expansion protocols are urgently needed effectively study disease progression and therapy responses. Here we present establishment long-term CTC-derived organoids from female metastatic Multiomics analysis along preclinical modeling xenografts identified neuregulin 1 (NRG1)–ERBB2 receptor tyrosine kinase 3 ( ERBB3 /HER3)...
Lifelong blood cell production is governed through the poorly understood integration of cell-intrinsic and -extrinsic control hematopoietic stem (HSC) quiescence activation. MicroRNAs (miRNAs) coordinately regulate multiple targets within signaling networks, making them attractive candidate HSC regulators. We report that miR-126, a miRNA expressed in early progenitors, plays pivotal role restraining cell-cycle progression vitro vivo. miR-126 knockdown by using lentiviral sponges increased...
// Irène Baccelli 1 , 2 * Albrecht Stenzinger 3 Vanessa Vogel Berit Maria Pfitzner 4 Corinna Klein Markus Wallwiener 5 Martina Scharpff Massimo Saini Tim Holland-Letz 6 Hans-Peter Sinn Andreas Schneeweiss Carsten Denkert 7 Wilko Weichert Trumpp Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany Divison of Cells Cancer, Deutsches Krebsforschungszentrum (DKFZ), Pathology, University Hospital 224, Charité...
Chemotherapy resistance resulting in incomplete pathologic response is associated with high risk of metastasis and early relapse breast cancer. The aim this study was to identify evaluate biomarkers treatment-resistant tumor cells. We performed a cell surface marker screen triple-negative cancer patient-derived xenograft models treated standard care genotoxic chemotherapy. Global expression profiling used further characterize the identified subpopulations. High sialyl-glycolipid...
The acquisition of mesenchymal traits is considered a hallmark breast cancer progression. However, the functional relevance epithelial-to-mesenchymal transition (EMT) remains controversial and context dependent. Here, we isolate epithelial populations from human metastatic biopsies assess their potential in vivo. Strikingly, progressively decreasing cell adhesion molecule (EPCAM) levels correlate with declining disease propagation. Mechanistically, find that persistent EPCAM expression marks...
Abstract Dynamic polarisation of tumour cells is essential for metastasis. While the role during dedifferentiation and migration well established, metastasising phases detachment has not been investigated. Here we identify characterise a type maintained by single in liquid phase termed single-cell (sc) polarity investigate its We demonstrate that sc an inherent feature from different entities observed circulating patients. Functionally, propose pole directly involved early attachment,...
The status of long-term quiescence and dormancy guarantees the integrity hematopoietic stem cells (HSCs) during adult homeostasis. However molecular mechanisms regulating HSC remain poorly understood. Here we show that cylindromatosis (CYLD), a tumor suppressor gene negative regulator NF-κB signaling with deubiquitinase activity, is highly expressed in label-retaining dormant HSCs (dHSCs). Moreover, Cre-mediated conditional elimination catalytic domain CYLD induced dHSCs to exit abrogated...
Abstract Despite important advances in the treatment of breast cancer, 5-year survival rate for patients with distant metastasis remains less than 30%. Metastasis is a complex, multi-step process beginning local invasion and ending outgrowth systemically disseminated cells into actively proliferating metastases that ultimately cause destruction vital organs. It this last step limits patient and, at same time, least understood mechanistically. Here, we focus on understanding determinants...
Breast tumors and their derived circulating cancer cells express the leukocyte β
Abstract We developed an autologous hematopoietic stem cell-based platform designed to deliver IFNa, by a transcriptional and post-transcriptional control mechanism mediated miRNA target sequences, specifically into the tumor microenvironment (TME) via Tie-2 expressing monocytes (Temferon). As of Feb 2022, 3 escalating doses Temferon (0.5-2.0x106/kg) were tested across 15 newly diagnosed, unmethylated MGMT GBM patients assigned 5 cohorts. Follow-up from surgery is 6–28mo (2–25mo after...