Sebastian Lange
A5039122987- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Gastric Cancer Management and Outcomes
- Neuroendocrine Tumor Research Advances
- Cancer Immunotherapy and Biomarkers
- Esophageal Cancer Research and Treatment
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Cancer Cells and Metastasis
- Hepatitis B Virus Studies
- Lung Cancer Treatments and Mutations
- Lung Cancer Research Studies
- Helicobacter pylori-related gastroenterology studies
- Colorectal Cancer Surgical Treatments
- Liver Disease Diagnosis and Treatment
- Colorectal Cancer Treatments and Studies
- vaccines and immunoinformatics approaches
- Neonatal Respiratory Health Research
- Renal cell carcinoma treatment
- Mathematical Biology Tumor Growth
- Colorectal Cancer Screening and Detection
- Cancer-related Molecular Pathways
- Virology and Viral Diseases
Technical University of Munich
2015-2025
Klinikum rechts der Isar
2015-2024
Heidelberg University
2024
German Cancer Research Center
2024
Molecular Oncology (United States)
2021-2022
Deutschen Konsortium für Translationale Krebsforschung
2022
Translational Research in Oncology
2021
München Klinik
2018
University Children's Hospital Tübingen
2013
Universitätsklinikum Tübingen
2011-2012
Here, we show CRISPR/Cas9-based targeted somatic multiplex-mutagenesis and its application for high-throughput analysis of gene function in mice. Using hepatic single guide RNA (sgRNA) delivery, large sets to induce hepatocellular carcinoma (HCC) intrahepatic cholangiocarcinoma (ICC). We observed Darwinian selection target genes, which suppress tumorigenesis the respective cellular/tissue context, such as Pten or Cdkn2a, conversely found low frequency Brca1/2 alterations, explaining...
Mouse transgenesis has provided fundamental insights into pancreatic cancer, but is limited by the long duration of allele/model generation. Here we show transfection-based multiplexed delivery CRISPR/Cas9 to pancreas adult mice, allowing simultaneous editing multiple gene sets in individual cells. We use method induce cancer and exploit mutational signatures for phylogenetic tracking metastatic disease. Our results demonstrate that CRISPR/Cas9-multiplexing enables key applications, such as...
Abstract Systemic pan-tumor analyses may reveal the significance of common features implicated in cancer immunogenicity and patient survival. Here, we provide a comprehensive multi-omics data set for 32 patients across 25 tumor types proteogenomic-based discovery neoantigens. By using an optimized computational approach, discover large number tumor-specific tumor-associated antigens. To create pipeline identification neoantigens our cohort, combine DNA RNA sequencing with MS-based...
(ATM) is the most frequently mutated DNA damage response gene, involved in homologous recombination (HR), pancreatic ductal adenocarcinoma (PDAC).
Abstract Dynamic polarisation of tumour cells is essential for metastasis. While the role during dedifferentiation and migration well established, metastasising phases detachment has not been investigated. Here we identify characterise a type maintained by single in liquid phase termed single-cell (sc) polarity investigate its We demonstrate that sc an inherent feature from different entities observed circulating patients. Functionally, propose pole directly involved early attachment,...
Abstract Evasion of programmed cell death represents a critical form oncogene addiction in cancer cells. Understanding the molecular mechanisms underpinning survival despite oncogenic stress could provide basis for potential therapeutic interventions. Here we explore role pro-survival genes integrity during clonal evolution non-small lung (NSCLC). We identify gains MCL-1 at high frequency multiple independent NSCLC cohorts, occurring both clonally and subclonally. Clonal loss functional TP53...
Report4 February 2022Open Access Source DataTransparent process Identification of treatment-induced vulnerabilities in pancreatic cancer patients using functional model systems Katja Peschke Medical Clinic and Polyclinic II, Klinikum rechts der Isar, Technical University Munich, München, Germany Contribution: Conceptualization, Formal analysis, Validation, Investigation, Methodology, Writing - original draft, review & editing Search for more papers by this author Hannah Jakubowsky Institute...
In patients with pancreatic ductal adenocarcinoma (PDAC), intratumoural and intertumoural heterogeneity increases chemoresistance mortality rates. However, such morphological phenotypic diversities are not typically captured by organoid models of PDAC. Here we show that branched organoids embedded in collagen gels can recapitulate the landscape seen murine human PDAC, pronounced molecular is governed defined transcriptional programmes (notably, epithelial-to-mesenchymal plasticity),...
One of the major challenges in using pancreatic cancer patient–derived organoids (PDOs) precision oncology is time from biopsy to functional characterization. This particularly true for endoscopic ultrasound-guided fine-needle aspiration biopsies, typically resulting specimens with limited tumor cell yield. Here, we tested conditioned media individual PDOs cell-free DNA detect driver mutations already early on during expansion process accelerate genetic characterization as well subsequent...
The 2019 World Health Organization (WHO) classification of colorectal carcinoma (CRC) profoundly reclassified CRC subtypes and introduces tumor budding as a second major grading criterion, while condensing conventional grade into 2-tiered system. So far it remains largely unexplored how these parameters interact with each other whether they truly have an independent impact on patient prognosis. We large single-center cohort 1004 CRCs spanning 2 decades for adjusted WHO (low vs. high),...
Abstract Background In neuro-oncology, the inclusion of tumor patients in molecular board has only become increasingly widespread recent years, but so far there are no standards for indication, procedure, evaluation, therapy recommendations and implementation neuro-oncological patients. The present work examines current handling included boards Germany. Methods We created an online based survey with questions covering neuro-oncologic patient inclusion, annotation genetic analyses, management...
Although most hepatocellular carcinoma (HCC) cases are driven by hepatitis and cirrhosis, a subset of patients with chronic B develop HCC in the absence advanced liver disease, indicating oncogenic potential virus (HBV). We investigated role HBV transcripts proteins on development inflammation HBV-transgenic mice.
Cholangiocarcinoma (CC) is curable only in early stages by complete surgical resection. Thus, advanced disease which a removal of the tumor mass no longer possible and palliative chemotherapy achieves modest success, therapeutics employing new methods action are desperately needed. Oncolytic viruses employed clinical studies have been shown to spread preferentially cancer cells. Beyond that, virotherapeutic cell killing can be enhanced virus-based expression suicide genes. We engineered...
The molecular carcinogenesis of intraductal tubulopapillary neoplasms (ITPN), recently described as rare in the pancreato-biliary tract with a favorable prognosis despite high incidence associated adenocarcinoma, is still poorly understood. To identify driver genes, chromosomal gains and losses, mutational signatures, key signaling pathways, potential therapeutic targets, profile 11 biliary 6 pancreatic ITPNs, invasive adenocarcinoma 14/17 cases, are studied by whole exome sequencing (WES)....
Background: Colorectal mixed adenoneuroendocrine carcinomas (MANECs) are clinically highly aggressive neoplasms. MANECs composed of variable adenocarcinoma components combined with morphologically distinct neuroendocrine carcinoma components, which confirmed by synaptophysin immunohistochemistry, the gold standard marker a differentiation. However, biological behavior adenocarcinomas that express but do not show typical morphology remains unclear. Methods: We investigated expression in 1002...