A5082134039- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- Protein Structure and Dynamics
- Virus-based gene therapy research
- Manufacturing Process and Optimization
- Immunotherapy and Immune Responses
- Chromosomal and Genetic Variations
- SARS-CoV-2 and COVID-19 Research
- Ubiquitin and proteasome pathways
- Connective tissue disorders research
- Phytochemicals and Antioxidant Activities
- Flexible and Reconfigurable Manufacturing Systems
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Wnt/β-catenin signaling in development and cancer
- Glycosylation and Glycoproteins Research
- Knowledge Management and Sharing
- Antioxidant Activity and Oxidative Stress
- COVID-19 Clinical Research Studies
- Cancer-related molecular mechanisms research
- Assembly Line Balancing Optimization
- Religion, Theology, and Education
Universitätsklinikum Würzburg
2023
University Medical Center Freiburg
2022
University of Freiburg
2022
Technical University of Munich
2015-2022
Technische Hochschule Ulm
2021
Klinikum rechts der Isar
2015-2017
Imperial College London
2017
German Cancer Research Center
2015
Heidelberg University
2015
Here, we show CRISPR/Cas9-based targeted somatic multiplex-mutagenesis and its application for high-throughput analysis of gene function in mice. Using hepatic single guide RNA (sgRNA) delivery, large sets to induce hepatocellular carcinoma (HCC) intrahepatic cholangiocarcinoma (ICC). We observed Darwinian selection target genes, which suppress tumorigenesis the respective cellular/tissue context, such as Pten or Cdkn2a, conversely found low frequency Brca1/2 alterations, explaining...
Mouse transgenesis has provided fundamental insights into pancreatic cancer, but is limited by the long duration of allele/model generation. Here we show transfection-based multiplexed delivery CRISPR/Cas9 to pancreas adult mice, allowing simultaneous editing multiple gene sets in individual cells. We use method induce cancer and exploit mutational signatures for phylogenetic tracking metastatic disease. Our results demonstrate that CRISPR/Cas9-multiplexing enables key applications, such as...
Abstract Immunization with two mRNA vaccine doses elicits robust spike-specific CD8 + T cell responses, but reports of waning immunity after COVID-19 vaccination prompt the introduction booster campaigns. However, effect on response remains unclear. Here we show that cells are activated and expanded in all analyzed individuals receiving 3 rd 4 th shots. This boost is followed by a contraction phase lasts only for about 30-60 days. The memory stem pool not affected vaccination. Both...
Lung diseases impose a huge economic and health burden worldwide. A key aspect of several adult lung diseases, such as idiopathic pulmonary fibrosis (IPF) chronic obstructive disease (COPD), including emphysema, is aberrant tissue repair, which leads to an accumulation damage impaired respiratory function. Currently, there are few effective treatments available for these their incidence rising. The planar cell polarity (PCP) pathway critical the embryonic development many organs, kidney...
Abstract The aim of the present study was to systematically investigate possibilities stabilizing a bulk oil rich in long‐chain polyunsaturated fatty acids under ambient conditions. Combinations different antioxidants (α‐, γ‐ and/or δ‐tocopherol, rosmarinic acid and rosemary extract carnosic acid) as well lecithin citric were investigated. Efficient stabilization achieved by choosing combination tocopherols or δ‐tocopherol low α‐tocopherol, including tocopherol‐sparing synergists like...
Genetically engineered mouse models (GEMM) are an elegant tool to study liver carcinogenesis in vivo. Newly designed need detailed (histopathological) phenotyping when described for the first time avoid misinterpretation and misconclusions. Many chemically induced hepatocarcinogenesis comprise a huge variety of histologically benign malignant neoplastic, as well non-neoplastic, lesions. Such comprehensive categorization data GEMM still missing. In this study, 874 microscopically categorized...
This paper presents a new multi-criteria evaluation method in the field of assembly system design using graph-based languages. After introducing concept planning phase design, this contribution illustrates current approaches and criteria to evaluate results process. Afterwards, mathematical principles optimization are portrayed. The newly developed as core basis for decision making combines advantages two approaches: manufacturing value well meta-objective function. information-technical...