Fengtang Yang
A5008181388- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Genetic Mapping and Diversity in Plants and Animals
- Genomic variations and chromosomal abnormalities
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- DNA Repair Mechanisms
- Evolution and Paleontology Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Bat Biology and Ecology Studies
- Glioma Diagnosis and Treatment
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- Pluripotent Stem Cells Research
- Digestive system and related health
- Advanced biosensing and bioanalysis techniques
- Genetic and phenotypic traits in livestock
- RNA and protein synthesis mechanisms
- Sperm and Testicular Function
- Genomics and Rare Diseases
- Epigenetics and DNA Methylation
- Prenatal Screening and Diagnostics
Shandong University of Technology
2022-2025
Xi'an Jiaotong University
2025
First Hospital of Shanxi Medical University
2025
Shanxi Medical University
2025
Wellcome Sanger Institute
2014-2024
Hainan University
2024
Nantong University
2024
GS Engineering (United States)
2024
Ministry of Industry and Information Technology
2024
Beijing Institute of Technology
2024
Cancer is driven by somatically acquired point mutations and chromosomal rearrangements, conventionally thought to accumulate gradually over time. Using next-generation sequencing, we characterize a phenomenon, which term chromothripsis, whereby tens hundreds of genomic rearrangements occur in one-off cellular crisis. Rearrangements involving one or few chromosomes crisscross back forth across involved regions, generating frequent oscillations between two copy number states. These hallmarks...
Structural variation of the genome involves kilobase- to megabase-sized deletions, duplications, insertions, inversions, and complex combinations rearrangements. We introduce high-throughput massive paired-end mapping (PEM), a large-scale genome-sequencing method identify structural variants (SVs) approximately 3 kilobases (kb) or larger that combines rescue capture paired ends 3-kb fragments, 454 sequencing, computational approach map DNA reads onto reference genome. PEM was used SVs in an...
The cancer genome is moulded by the dual processes of somatic mutation and selection. Homozygous deletions in genomes occur over recessive genes, where they can confer selective growth advantage, fragile sites, are thought to reflect an increased local rate DNA breakage. However, most homozygous unexplained. Here we identified 2,428 746 cell lines. These overlie 11% protein-coding genes that, therefore, not mandatory for survival human cells. We derived structural signatures that distinguish...
In search of vascular smooth muscle cell differentiation markers, we identified two genes encoding members a new family type II integral membrane proteins. Both are ubiquitously expressed, and tissue-specific alternative mRNA initiation splicing generate at least major isoforms each protein, with the smaller being truncated N-terminus. We have named these proteins nesprin-1 -2 for nuclear envelope spectrin repeat, as they characterized by presence multiple, clustered repeats, bipartite...
Stem cells hold great potential as cell-based therapies to promote vascularization and tissue regeneration. However, the use of stem alone angiogenesis remains limited because insufficient expression angiogenic factors low cell viability after transplantation. Here, we have developed vascular endothelial growth factor (VEGF) high-expressing, transiently modified for purposes promoting angiogenesis. Nonviral, biodegradable polymeric nanoparticles were deliver hVEGF gene human mesenchymal...
We report full-length draft de novo genome assemblies for 16 widely used inbred mouse strains and find extensive strain-specific haplotype variation. identify characterize 2,567 regions on the current reference exhibiting greatest sequence diversity. These are enriched genes involved in pathogen defence immunity exhibit enrichment of transposable elements signatures recent retrotransposition events. Combinations alleles unique to an individual strain commonly observed at these loci,...
Abstract The National Genomics Data Center (NGDC), part of the China for Bioinformation (CNCB), provides a suite database resources to support worldwide research activities in both academia and industry. With explosive growth multi-omics data, CNCB-NGDC is continually expanding, updating enriching its core through big data deposition, integration translation. In past year, considerable efforts have been devoted 2019nCoVR, newly established resource providing global landscape SARS-CoV-2...
Here, we show CRISPR/Cas9-based targeted somatic multiplex-mutagenesis and its application for high-throughput analysis of gene function in mice. Using hepatic single guide RNA (sgRNA) delivery, large sets to induce hepatocellular carcinoma (HCC) intrahepatic cholangiocarcinoma (ICC). We observed Darwinian selection target genes, which suppress tumorigenesis the respective cellular/tissue context, such as Pten or Cdkn2a, conversely found low frequency Brca1/2 alterations, explaining...
Mouse transgenesis has provided fundamental insights into pancreatic cancer, but is limited by the long duration of allele/model generation. Here we show transfection-based multiplexed delivery CRISPR/Cas9 to pancreas adult mice, allowing simultaneous editing multiple gene sets in individual cells. We use method induce cancer and exploit mutational signatures for phylogenetic tracking metastatic disease. Our results demonstrate that CRISPR/Cas9-multiplexing enables key applications, such as...
Mutations in the ATM tumor suppressor gene confer hypersensitivity to DNA-damaging chemotherapeutic agents. To explore genetic resistance mechanisms, we performed genome-wide CRISPR-Cas9 screens cells treated with DNA topoisomerase I inhibitor topotecan. Thus, here establish that inactivating terminal components of non-homologous end-joining (NHEJ) machinery or BRCA1-A complex specifically topotecan ATM-deficient cells. We show ATM-mutant poly-(ADP-ribose) polymerase (PARP) olaparib reflects...
Piggybacking on Cancer Genes Transposons are mobile segments of DNA that can insert in or near important genes to cause mutations disrupt gene function. Rad et al. (p. 1104 , published online 14 October) adapted a mutagenic transposon called Piggybac, originally derived from moth, into tool for discovery cancer-causing mice. Mobilization Piggybac mice was associated with the development leukemias and solid tumors. In many instances causative mutations, which were identified by mapping...
Abstract Silencing and variegated transgene expression are poorly understood problems that can interfere with gene function studies in human embryonic stem cells (hESCs). We show (enhanced green fluorescent protein [EGFP]) from random integration sites hESCs is affected by variegation silencing, only half of expressing the transgene, which gradually lost after withdrawal selection differentiation. tested hypothesis a integrated into adeno-associated virus type 2 (AAV2) target region on...