A5090548592- Gut microbiota and health
- 3D Printing in Biomedical Research
- Gastrointestinal motility and disorders
- RNA Interference and Gene Delivery
- Probiotics and Fermented Foods
- Cellular Mechanics and Interactions
- Clostridium difficile and Clostridium perfringens research
- Electrospun Nanofibers in Biomedical Applications
- Proteins in Food Systems
- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- CAR-T cell therapy research
- Nanoparticle-Based Drug Delivery
- Nanofabrication and Lithography Techniques
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Pancreatic function and diabetes
- Colorectal Cancer Screening and Detection
- Diverticular Disease and Complications
- Mesenchymal stem cell research
- Hip disorders and treatments
- Microfluidic and Bio-sensing Technologies
- Environmental DNA in Biodiversity Studies
- Polymer Surface Interaction Studies
- Advanced biosensing and bioanalysis techniques
California Institute of Technology
2014-2023
Division of Chemistry
2020
Massachusetts Institute of Technology
2008-2012
Boston Children's Hospital
2011
Yonsei University
2010
Robert Koch Institute
2010
Stanford University
2010
Moscow State University of Medicine and Dentistry
2009
Stem cells hold great potential as cell-based therapies to promote vascularization and tissue regeneration. However, the use of stem alone angiogenesis remains limited because insufficient expression angiogenic factors low cell viability after transplantation. Here, we have developed vascular endothelial growth factor (VEGF) high-expressing, transiently modified for purposes promoting angiogenesis. Nonviral, biodegradable polymeric nanoparticles were deliver hVEGF gene human mesenchymal...
The targeted delivery of therapeutics to tumors remains an important challenge in cancer nanomedicine. Attaching nanoparticles cells that have tumoritropic migratory properties is a promising modality address this challenge. Here we describe technique create nanoparticulate cellular patches remain attached the membrane for up 2 days. NeutrAvidin-coated were anchored on possessing biotinylated plasma membrane. Human bone marrow derived mesenchymal stem with retained their inherent as shown...
A fundamental goal in microbiome studies is determining which microbes affect host physiology. Standard methods for changes microbial taxa measure relative, rather than absolute abundances. Moreover, often analyze only stool, despite diversity differing substantially among gastrointestinal (GI) locations. Here, we develop a quantitative framework to abundances of individual bacterial by combining the precision digital PCR with high-throughput nature 16S rRNA gene amplicon sequencing. In...
Abstract Background The upper gastrointestinal tract plays a prominent role in human physiology as the primary site for enzymatic digestion and nutrient absorption, immune sampling, drug uptake. Alterations to small intestine microbiome have been implicated various diseases, such non-alcoholic steatohepatitis inflammatory bowel conditions. Yet, physiological functional roles of microbiota humans remain poorly characterized because complexities associated with its sampling. Rodent models are...
An integrated high-throughput polymer synthesis and rapid material/protein/cell interaction assays were developed to optimize stem cell microenvironments. Microarrayed polymers synthesized studied for the ability support growth of partially differentiated human embryonic cells. In parallel, a programmed laser scanning cytometry system was allow quantification material interaction. Detailed facts importance specialist readers are published as "Supporting Information". Such documents...
Embryonic stem cells (ESCs) are promising cell sources for tissue engineering and regenerative medicine. Scaffolds ESC-based regeneration should provide not only structural support, but also signals capable of supporting appropriate differentiation development. Extracellular matrix (ECM) is a key component the niche in vivo can influence fate via mediating attachment migration, presenting chemical physical cues, as well binding soluble factors. Here we investigated effects combinatorial...
Multistability and hysteresis is a potential mechanism to describe shifts persistence of aerobe-anaerobe communities.
Here we develop nanoparticles composed of lipid-like materials (lipidoids) to facilitate non-viral delivery small interfering RNA (siRNA) endothelial cells (ECs). Nanoparticles siRNA and lipidoids with size (~200 nm) positive charge (~34 mV) were formed by self assembly siRNA. Ten synthesized screened for their ability the into ECs. Particles leading show significantly better ECs than a commercially-available transfection reagent, Lipofectamine 2000. As model potential therapeutic...
Microscale direct writing (MDW) technology is developed for the generation of complex extracellular Matrix (ECM) protein arrays at subcellular feature size with multiple components. Cell-compatible, two-component ECM a 6–9 micrometers (see image) are systematically generated varying spacing and composition, their effects on cellular attachment spreading model cell line, mouse myofibroblasts, evaluated.
Abstract A fundamental goal in microbiome studies is to determine which microbes affect host physiology. Standard methods for determining changes microbial taxa measure relative abundances, cannot capture absolute changes. Moreover, often focus on a single site (usually stool), although demographics differ substantially among gastrointestinal (GI) locations. Here, we developed quantitative framework accurately abundances of individual bacterial by combining the precision digital PCR with...
ABSTRACT Current methods for detecting, accurately quantifying, and profiling complex microbial communities based on the 16S rRNA marker genes are limited by a number of factors, including inconsistent extraction nucleic acids, amplification interference from contaminants host DNA, different coverage PCR primers utilized quantification sequencing, potentially biases in rates among taxa during amplicon barcoding. Here, we describe single-step method that enables gene DNA copies with wide...
The lumen of the small intestine (SI) is filled with particulates: microbes, therapeutic particles, and food granules. structure this particulate suspension could impact uptake drugs nutrients function microorganisms; however, little understood about how re-structured as it transits gut. Here, we demonstrate that particles spontaneously aggregate in SI luminal fluid ex vivo. We find mucins immunoglobulins are not required for aggregation. Instead, aggregation can be controlled using polymers...