A5043187815- Osteoarthritis Treatment and Mechanisms
- Mesenchymal stem cell research
- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- RNA Interference and Gene Delivery
- Knee injuries and reconstruction techniques
- Periodontal Regeneration and Treatments
- Cancer Cells and Metastasis
- Silk-based biomaterials and applications
- Orthopaedic implants and arthroplasty
- Proteoglycans and glycosaminoglycans research
- Pluripotent Stem Cells Research
- Bone fractures and treatments
- Cell Adhesion Molecules Research
- Connective tissue disorders research
- Total Knee Arthroplasty Outcomes
- Angiogenesis and VEGF in Cancer
- Polymer Surface Interaction Studies
- Orthopedic Infections and Treatments
- Extracellular vesicles in disease
- Virus-based gene therapy research
- Hydrogels: synthesis, properties, applications
Stanford University
2016-2025
Palo Alto University
2017-2024
Stratford University
2024
Nanjing Institute of Railway Technology
2024
Uppsala University
2024
Sir Run Run Shaw Hospital
2023
Henan University of Science and Technology
2020-2023
Ningxia Water Conservancy
2023
Zhejiang University
2023
Second Xiangya Hospital of Central South University
2023
Shear-thinning, self-healing hydrogels are promising vehicles for therapeutic cargo delivery due to their ability be injected using minimally invasive surgical procedures. We present an injectable hydrogel a novel combination of dynamic covalent crosslinking with thermoresponsive engineered proteins.
Glioblastoma (GBM) is the most common and aggressive form of primary brain tumor with a median survival 12-15 months, mechanisms underlying GBM progression remain largely elusive. Given importance niche signaling in driving progression, there strong need to develop vitro models facilitate analysis cell-niche interactions physiologically relevant controllable manner. Here we report development bioengineered 3D model help elucidate effects matrix stiffness on cell fate using...
Abstract The biofabrication of three-dimensional (3D) tissues that recapitulate organ-specific architecture and function would benefit from temporal spatial control cell-cell interactions. Bioprinting, while potentially capable achieving such control, is poorly suited to organoids with conserved cytoarchitectures are susceptible plastic deformation. Here, we develop a platform, termed Spatially Patterned Organoid Transfer (SPOT), consisting an iron-oxide nanoparticle laden hydrogel...
Apert syndrome is an autosomal dominant disorder characterized by malformations of the skull, limbs and viscera. Two-thirds affected individuals have a S252W mutation in fibroblast growth factor receptor 2 (FGFR2). To study pathogenesis this condition, we generated knock-in mouse model with mutation. The Fgfr2(+/S252W) mutant mice abnormalities skeleton, as well other organs including brain, thymus, lungs, heart intestines. In neurocranium, found midline sutural defect craniosynostosis...
Bioresponsive and intelligent biomaterials are a vehicle for manipulating cell function to promote tissue development and/or engineering. A photopolymerized hydrogel based on phosphoester– poly(ethylene glycol) polymer (PhosPEG) was synthesized application marrow-derived mesenchymal stem (MSC) encapsulation engineering of bone. The phosphor-containing hydrogels were hydrolytically degradable the rate degradation increased in presence bone-derived enzyme, alkaline phosphatase. Gene expression...
Stem cell-based bone tissue engineering with adipose-derived stromal cells (ASCs) has shown great promise for revolutionizing treatment of large deficits. However, there is still a lack consensus on cell surface markers identifying osteoprogenitors. Fluorescence-activated sorting identified subpopulation CD105(low) enhanced osteogenic differentiation. The purpose the present study was to compare ability CD90 (Thy-1) identify osteoprogenitors relative CD(105).Unsorted cells, CD90(+), CD90(-),...
Since its introduction in the early 1990s, layer-by-layer (LbL) self-assembly of films has been widely used fields nanoelectronics, optics, sensors, surface coatings, and controlled drug delivery. The growth this industry is propelled by ease film manufacture, low cost, mild assembly conditions, precise control coating thickness, versatility materials. Despite wealth research on LbL for biomolecule delivery, clinical translation limited slow. This review provides an overview methods...
Human induced pluripotent stem cells (hiPSCs) have demonstrated great potential for hyaline cartilage regeneration. However, current approaches chondrogenic differentiation of hiPSCs are complicated and inefficient primarily due to intermediate embryoid body formation, which is required generate endodermal, ectodermal, mesodermal cell lineages. We report a new, straightforward highly efficient approach hiPSCs, avoids formation. differentiated directly into mesenchymal /stromal (MSC)...
Diabetes mellitus (DM) is a metabolic disease frequently associated with impaired bone healing. Despite its increasing prevalence worldwide, the molecular etiology of DM-linked skeletal complications remains poorly defined. Using advanced stem cell characterization techniques, we analyzed intrinsic and extrinsic determinants mouse (mSSC) function to identify specific mSSC niche-related abnormalities that could impair repair in diabetic (Db) mice. We discovered high serum concentrations tumor...
Significance Current treatment for glioblastoma multiforme (GBM) fails to address its highly infiltrative nature; often leaves behind microscopic neoplastic satellites, resulting in eventual tumor recurrence. Here we report polymeric nanoparticle-engineered human adipose-derived stem cells (hADSCs) overexpressing the cancer-specific TNF-related apoptosis-inducing ligand targeting and eradicating cells. Engineered hADSCs exhibited long-range directional migration toward patient-derived GBM...
Abstract Naturally‐derived cell membranes have shown great promise in functionalizing nanoparticles to enhance biointerfacing functions for drug delivery applications. However, its potential macroporous scaffolds tissue regeneration vivo remains unexplored. Engineering with immunomodulatory represents an exciting strategy but is largely limited soft tissues. Critical‐sized bone defects cannot heal on their own, and the role of adaptive immune cells scaffold‐mediated healing cranial unknown....
Glioblastoma multiforme (GBM) progression is associated with changes in matrix stiffness, and different regions of the tumor niche exhibit distinct stiffnesses. Using elastic hydrogels, previous work has demonstrated that stiffness modulates GBM behavior drug responses. However, brain tissue viscoelastic, how impacts invasive phenotype response to therapy within a viscoelastic remains largely unclear. Here, we report three-dimensional (3D) hydrogel system models heterogeneity present niche....
Angiogenesis is essential to wound repair, and vascular endothelial growth factor (VEGF) a potent stimulate angiogenesis. Here, we examine the potential of VEGF-overexpressing adipose-derived stromal cells (ASCs) for accelerating healing using nonviral, biodegradable polymeric vectors. Mouse ASCs were transfected with DNA plasmid encoding VEGF or green fluorescent protein (GFP) poly (β-amino) esters (PBAE). Cells Lipofectamine 2000, commercially available transfection reagent, included as...