A5071612299- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Xenotransplantation and immune response
- Virus-based gene therapy research
- Botulinum Toxin and Related Neurological Disorders
- Reproductive Physiology in Livestock
- Renal and related cancers
- Epigenetics and DNA Methylation
- Cellular transport and secretion
- Sperm and Testicular Function
- Glycosylation and Glycoproteins Research
- Prenatal Screening and Diagnostics
- Monoclonal and Polyclonal Antibodies Research
- Tissue Engineering and Regenerative Medicine
- Genetic and phenotypic traits in livestock
- Animal Virus Infections Studies
- Ovarian function and disorders
- Toxin Mechanisms and Immunotoxins
- Molecular Biology Techniques and Applications
- Neurological disorders and treatments
- Biochemical and Structural Characterization
- Bacteriophages and microbial interactions
- Lipid Membrane Structure and Behavior
Medizinische Hochschule Hannover
2014-2024
Friedrich-Loeffler-Institut
2013-2023
Center for Experimental and Clinical Infection Research
2021
Institut für Tier-, Natur- und Umweltethik
1993-2020
Schön Klinik Neustadt
2014
Schreiner Group (Germany)
2007-2009
Research Institute for Farm Animal Biology (FBN)
2008
University of Veterinary Medicine Hannover, Foundation
2007
Homerton University Hospital
2007
Faktor 10 – Institut für nachhaltiges Wirtschaften gemeinnützige
1998-2006
Clostridial neurotoxins, tetanus toxin (TeTx) and the seven related but serologically distinct botulinal neurotoxins (BoNT/A to BoNT/G), are potent inhibitors of synaptic vesicle exocytosis in nerve endings. Recently it was reported that light chains clostridial act as zinc-dependent metalloproteases which specifically cleave target proteins such synaptobrevin/VAMPs, HPC-1/syntaxin (BoNT/C1), SNAP-25 (BoNT/A). We show here BoNT/E, like BoNT/A, cleaves SNAP-25, generated by vitro translation...
This study investigated the capacity of healthy oocytes derived from follicles different size to undergo normal fertilization and early embryonic development in vitro full-term vivo. Ovaries were collected a local abattoir dissected classified as follows: group A, greater than 4-8 mm (large); B, 2-4 (medium); C, 1-2 (small). Oocytes isolated by puncturing follicular wall pressing follicle. Only healthy-looking cumulus-oocyte complexes (COC) used for maturation. fertilized frozen/thawed semen...
The large offspring syndrome (LOS) is observed in bovine and ovine following transfer of vitro-produced (IVP) or cloned embryos characterized by a multitude pathologic changes, which extended gestation length increased birthweight are predominant features. In the present study, we used blastocysts to analyze cellular parameters, i.e., number cells Day 7 size 12 elongating blastocysts, molecular relative abundance developmentally important genes: glucose transporter (Glut) 1, Glut-2, Glut-3,...
Zinc-finger nucleases (ZFNs) are powerful tools for producing gene knockouts (KOs) with high efficiency. Whereas ZFN-mediated disruption has been demonstrated in laboratory animals such as mice, rats, and fruit flies, ZFNs have not used to disrupt an endogenous any large domestic species. Here we induce a biallelic knockout of the porcine α1,3-galactosyltransferase ( GGTA1 ) gene. Primary fibroblasts were treated designed against region coding catalytic core , resulting ∼1% ZFN-treated...
Most human embryos are aneuploid. Aneuploidy frequently arises during the early mitotic divisions of embryo, but its origin remains elusive. Human zygotes that cluster their nucleoli at pronuclear interface thought to be more likely develop into healthy euploid embryos. Here, we show parental genomes with in each pronucleus within and bovine zygotes, clustering is required for reliable unification after fertilization. During migration intact pronuclei, polarize toward other a process driven...
POU transcription factors are involved in transcriptional regulation during early embryonic development and cell differentiation. Oct-4, a member of this family, has been shown to be under strict murine development. The expression Oct-4 correlates with the undifferentiated phenotype mouse preimplantation embryo. In study, gene construct consisting selected parts region upstream from as promoter/enhancer, enhanced green fluorescent protein (EGFP) reporter five exons (GOF18-ΔPE EGFP) was...
Tetanus toxin is a potent neurotoxin that inhibits the release of neurotransmitters from presynaptic nerve endings. The mature composed heavy and light chain are linked via disulfide bridge. After entry tetanus into cytoplasm, released causes block neurotransmitter release. Recent evidence suggests L-chain may act as metalloendoprotease. Here we demonstrate blockade neurotransmission by in isolated terminals associated with selective proteolysis synaptobrevin, an integral membrane protein...
The importance of soluble N-ethyl maleimide (NEM)-sensitive fusion protein (NSF) attachment (SNAP) receptors (SNAREs) in synaptic vesicle exocytosis is well established because it has been demonstrated that clostridial neurotoxins (NTs) proteolyze the SNAREs (v-SNAREs) vesicle-associated membrane (VAMP)/brevins and their partners, target (t-SNAREs) syntaxin 1 SNAP25. Yet, several exocytotic events, including apical epithelial cells, are insensitive to numerous NTs, suggesting presence...
The seven serologically different botulinum neurotoxins are highly potent protein toxins that inhibit neurotransmitter release from peripheral cholinergic synapses. activated consist of the toxifying A-subunits (Mr approximately 50,000) linked by a disulfide bond to receptor-binding BC-subunits 100,000). We have established complete sequence neurotoxin type A (BoNT/A; 1,296 amino acid residues, Mr = 149,425) and partial E (273 residues) as deduced corresponding nucleotide sequences...
Cellubrevin is a member of the synaptobrevin/VAMP family SNAREs, which has broad tissue distribution. In fibroblastic cells it concentrated in vesicles recycle transferrin receptors but its role membrane trafficking and fusion remains to be demonstrated. Cellubrevin, like synaptic vesicle proteins synaptobrevins I II, can cleaved by tetanus toxin, metallo-endoprotease blocks neurotransmitter release. However, nonneuronal are unaffected toxin due lack cell surface for heavy chain. To...
In the eukaryotic cell, both secreted and plasma membrane proteins are synthesized at endoplasmic reticulum, then transported, via Golgi complex, to cell surface. Each of compartments this transport pathway carries out particular metabolic functions, therefore presumably contains a distinct complement proteins. Thus, mechanisms must exist for localizing such their respective destinations. However, major obstacle study is that isolation detailed analysis internal pose formidable technical...
The enzyme telomerase is active in germ cells and early embryonic development crucial for the maintenance of telomere length. Whereas different length telomeres somatic well documented, information on regulation during embryogenesis lacking. In this study, we demonstrate a elongation program at transition from morula to blastocyst mice cattle that establishes specific set point embryogenesis. We show process restores cloned embryos derived fibroblasts, regardless donor nuclei, stage...
Equal expression of X-linked genes such as G6PD and PGK in females males the initiation X-chromosome inactivation are critically dependent on X-inactive specific transcript (Xist). The objective present study was to determine effects vitro production (IVP) nuclear transfer (NT) relative abundance (RA) transcripts G6PD, PGK, Xist preimplantation bovine embryos. In experiment 1, sex-determined IVP or vivo-produced embryos were analyzed for mRNA 3 genes. sex ratio 36% vs. 64% blastocysts thus...
Abstract : Tetanus toxin and the seven serologically distinct botulinal neurotoxins (BoNT/A to BoNT/G) abrogate synaptic transmission at nerve endings through action of their light chains (L chains), which proteolytically cleave VAMP (vesicle‐associated membrane protein)/synaptobrevin, SNAP‐25 (synaptosome‐associated protein 25 kDa), or syntaxin. BoNT/C was reported proteolyze both syntaxin SNAP‐25. Here, we demonstrate that cleavage occurs between Arg 198 Ala 199 , depends on presence...