A5018744564- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Lung Cancer Treatments and Mutations
- Cancer Immunotherapy and Biomarkers
- Esophageal Cancer Research and Treatment
- Genetic factors in colorectal cancer
- Cancer Cells and Metastasis
- Gastric Cancer Management and Outcomes
- Epigenetics and DNA Methylation
- Lung Cancer Research Studies
- Neuroendocrine Tumor Research Advances
- Radiomics and Machine Learning in Medical Imaging
- Peptidase Inhibition and Analysis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- RNA modifications and cancer
- Head and Neck Cancer Studies
- Sarcoma Diagnosis and Treatment
- Gastrointestinal Tumor Research and Treatment
- HER2/EGFR in Cancer Research
- Monoclonal and Polyclonal Antibodies Research
- Lung Cancer Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Immunotherapy and Immune Responses
- Renal cell carcinoma treatment
- Advanced Proteomics Techniques and Applications
Technical University of Munich
2016-2025
Heidelberg University
2016-2025
German Cancer Research Center
2016-2025
Deutschen Konsortium für Translationale Krebsforschung
2015-2024
Klinikum rechts der Isar
2016-2024
University Hospital Heidelberg
2013-2023
German Cancer Society
2016-2023
National Center for Tumor Diseases
2010-2022
RWTH Aachen University
2022
Essen University Hospital
2011-2022
PURPOSE Preclinical data suggest a contribution of the immune system to chemotherapy response. In this study, we investigated prespecified hypothesis that presence lymphocytic infiltrate in cancer tissue predicts response neoadjuvant chemotherapy. METHODS We intratumoral and stromal lymphocytes total 1,058 pretherapeutic breast core biopsies from two anthracycline/taxane-based studies (GeparDuo, n = 218, training cohort; GeparTrio, 840, validation cohort). Molecular parameters lymphocyte...
Since the introduction of positron emission tomography (PET) imaging with 68Ga-PSMA-HBED-CC (=68Ga-DKFZ-PSMA-11), this method has been regarded as a significant step forward in diagnosis recurrent prostate cancer (PCa). However, published data exist for small patient cohorts only. The aim evaluation was to analyse diagnostic value 68Ga-PSMA-ligand PET/CT large cohort and influence several possibly interacting variables. We performed retrospective analysis 319 patients who underwent from 2011...
Purpose Our aim was to analyze and validate the prognostic impact of novel International Association for Study Lung Cancer (IASLC)/American Thoracic Society (ATS)/European Respiratory (ERS) proposal an architectural classification invasive pulmonary adenocarcinomas (ADCs) across all tumor stages. Patients Methods The pattern a large cohort 500 patients with resected ADCs (stages I IV) retrospectively analyzed in 5% increments classified according their predominant architecture (lepidic,...
High activity of histone deacetylases (HDACs) causes epigenetic alterations associated with malignant cell behaviour. Consequently, HDAC inhibitors have entered late-phase clinical trials as new antineoplastic drugs. However, little is known about expression and function specific isoforms in human tumours including prostate cancer. We investigated the class I HDACs 192 carcinomas by immunohistochemistry correlated our findings to clinicopathological parameters follow-up data. Class were...
The aim of this study was to develop a treatment protocol for <sup>225</sup>Ac-PSMA-617 α-radiation therapy in advanced-stage, metastatic castration-resistant prostate cancer patients with prostate-specific membrane antigen (PSMA)–positive tumor phenotype. <b>Methods:</b> A dosimetry estimate calculated on the basis time–activity curves derived from serially obtained <sup>177</sup>Lu-PSMA-617 scans extrapolated physical half-life <sup>225</sup>Ac, assuming instant decay unstable daughter...
Altered bacterial diversity of the intestinal mucosa-associated microbiota may reflect net influence lifestyle factors associated with development chronic inflammatory bowel diseases (IBD). While a reduced has been reported in IBD, little is known about fungal microbiota. The aim this study was to carry out systematic analysis IBD.The 104 colonic biopsy tissues from 47 controls and 57 IBD patients investigated using metagenomic 18S rDNA-based denaturing gradient gel electrophoresis (DGGE),...
Abstract Metabolites are the end products of cellular regulatory processes, and their levels can be regarded as ultimate response biological systems to genetic or environmental changes. We have used a metabolite profiling approach test hypothesis that quantitative signatures primary metabolites characterize molecular changes in ovarian tumor tissues. Sixty-six invasive carcinomas nine borderline tumors ovary were analyzed by gas chromatography/time-of-flight mass spectrometry (GC-TOF MS)...
Recently, several studies reported a strong functional link between histone deacetylases (HDAC) and the development of tumors large intestine. However, despite importance these molecules, comparably little is known on expression patterns functions specific HDAC isoforms in colorectal cancer.We characterized class I isoform cohort 140 carcinomas by immunohistochemistry. In addition, effects inhibition valproic acid suberoylanilide hydroxamic acid, knockdown short interfering RNA, were...
Apart from genetic alterations, development and progression of colorectal cancer has been linked to influences nutritional intake, hyperalimentation, cellular metabolic changes that may be the basis for new diagnostic therapeutic approaches. However, in contrast genomics proteomics, comprehensive metabolomic investigations alterations malignant tumors have rarely conducted. In this study we investigated a set paired samples normal colon tissue with gas-chromatography time-of-flight...
Human colon cancer harbors a small subfraction of tumor-initiating cells (TICs) that is assumed to be functionally homogeneous stem-cell-like population driving tumor maintenance and metastasis formation. We found unexpected cellular heterogeneity within the TIC compartment, which contains three types TICs. Extensively self-renewing long-term TICs (LT-TICs) maintained formation in serial xenotransplants. Tumor transient amplifying (T-TACs) with limited or no self-renewal capacity contributed...
// Jan Budczies 1,2,6,* , Moritz von Winterfeld 1,* Frederick Klauschen 1 Michael Bockmayr Jochen K. Lennerz 3 Carsten Denkert 1,6 Thomas Wolf 4,6 Arne Warth 4 Manfred Dietel Ioannis Anagnostopoulos Wilko Weichert 4,6,7 Daniel Wittschieber 5 and Albrecht Stenzinger Institute of Pathology, Charité University Hospital, Berlin, Germany 2 German Cancer Research Center (DKFZ), Heidelberg, Massachusetts General Hospital/Harvard Medical School, Department Boston, MA, USA Hospital Legal...
We hypothesized that the combination of oncolytic virotherapy with immune checkpoint modulators would reduce tumor burden by direct cell lysis and stimulate antitumor immunity. In this study, we have generated attenuated Measles virus (MV) vectors encoding antibodies against CTLA-4 PD-L1 (MV-aCTLA-4 MV-aPD-L1). characterized in terms growth kinetics, antibody expression, cytotoxicity vitro. Immunotherapeutic effects were assessed a newly established, fully immunocompetent murine model...
Leiomyosarcoma (LMS) is an aggressive mesenchymal malignancy with few therapeutic options. The mechanisms underlying LMS development, including clinically actionable genetic vulnerabilities, are largely unknown. Here we show, using whole-exome and transcriptome sequencing, that tumors characterized by substantial mutational heterogeneity, near-universal inactivation of TP53 RB1, widespread DNA copy number alterations chromothripsis, frequent whole-genome duplication. Furthermore, detect...
Biliary tract cancers (BTC) are relatively rare malignant tumours with poor prognosis. It is known from other solid neoplasms that antitumour inflammatory response has an impact on tumour behaviour and patient outcome. The aim of this study was to provide a comprehensive characterisation in human BTC. Tumour-infiltrating T lymphocytes (CD4+, CD8+, Foxp3+), natural killer cells (perforin+), B (CD20+), macrophages (CD68+) as well mast (CD117+) were assessed by immunohistochemistry 375 BTC...
Tumor spread, in general, is the most important factor determining outcome almost all malignant tumors. Lung tumors are unique with respect to potential routes for tumor dissemination, as apart from vascular, nodal, and distant spread of cells, through air spaces (STAS) might also occur. However, morphologic criteria STAS its prognostic impact have not been defined yet. We evaluated a series 569 resected pulmonary adenocarcinomas (ADCs) predefined limited extensive correlated our findings...
In breast cancer, the role of epigenetic alterations including modifications acetylation status histones in carcinogenesis has been an important research focus during last years. An increased deacetylation leads to cell proliferation, migration, angiogenesis and invasion. Class 1 histone deacetylases (HDAC) seem be most carcinogenesis.The immunhistochemical expression HDAC1, 2 3 was analyzed on tissue microarrays (TMAs) from 238 patients with primary cancer. We nuclear staining intensity...
Predicting the clinical outcome of cancer patients based on expression marker genes in their tumors has received increasing interest past decade. Accurate predictors and response to therapy could be used personalize thereby improve therapy. However, state art methods so far often found with limited prediction accuracy, reproducibility, unclear biological relevance. To address this problem, we developed a novel computational approach identify prognostic for that couples gene measurements from...
Abstract We used whole-genome and transcriptome sequencing to identify clinically actionable genomic alterations in young adults with pancreatic ductal adenocarcinoma (PDAC). Molecular characterization of 17 patients PDAC enrolled a precision oncology program revealed gene fusions amenable pharmacologic inhibition by small-molecule tyrosine kinase inhibitors all KRAS wild-type (KRASWT) tumors (4 17). These included recurrent NRG1 rearrangements predicted drive development through aberrant...