A5077422723- Metabolomics and Mass Spectrometry Studies
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Cancer Research and Treatments
- Computational Drug Discovery Methods
- Pancreatic and Hepatic Oncology Research
- Glycosylation and Glycoproteins Research
- Gut microbiota and health
- Pharmacogenetics and Drug Metabolism
- Monoclonal and Polyclonal Antibodies Research
- PARP inhibition in cancer therapy
- Zebrafish Biomedical Research Applications
- Ovarian cancer diagnosis and treatment
- Advanced Breast Cancer Therapies
- Cancer, Hypoxia, and Metabolism
- Pesticide and Herbicide Environmental Studies
- Pancreatic function and diabetes
- Reproductive Biology and Fertility
- Biochemical Analysis and Sensing Techniques
- Neuroendocrine Tumor Research Advances
- Traditional Chinese Medicine Studies
- Animal testing and alternatives
- Environmental Science and Water Management
- Clostridium difficile and Clostridium perfringens research
- Molecular Biology Techniques and Applications
BASF (Germany)
2022-2025
Robert Bosch (Germany)
2024
German Cancer Research Center
2015-2022
Heidelberg University
2015-2022
BASF (United States)
2022
Heidelberg Institute for Stem Cell Technology and Experimental Medicine
2015-2021
DKFZ-ZMBH Alliance
2015-2016
Article27 November 2017Open Access Transparent process Screening drug effects in patient-derived cancer cells links organoid responses to genome alterations Julia Jabs orcid.org/0000-0002-4915-9966 Division of Theoretical Bioinformatics, German Cancer Research Center (DKFZ), Heidelberg, Germany for Quantitative Analysis Molecular and Cellular Biosystems (BioQuant), University Department Bioinformatics Functional Genomics, Institute Pharmacy Biotechnology (IPMB) BioQuant, Heidelberg...
Chemotherapy resistance resulting in incomplete pathologic response is associated with high risk of metastasis and early relapse breast cancer. The aim this study was to identify evaluate biomarkers treatment-resistant tumor cells. We performed a cell surface marker screen triple-negative cancer patient-derived xenograft models treated standard care genotoxic chemotherapy. Global expression profiling used further characterize the identified subpopulations. High sialyl-glycolipid...
While grouping/read-across is widely used to fill data gaps, chemical registration dossiers are often rejected due weak category justifications based on structural similarity only. Metabolomics provides a route robust categories via evidence of shared molecular effects across source and target substances. To gain international acceptance, this approach must demonstrate high reliability, best-practice guidance required. The MetAbolomics ring Trial for CHemical groupING (MATCHING), comprising...
Abstract The gut microbiome catalyzes biotransformation reactions that influence intestinal absorption as a basis of microbiome-host interactions. A better understanding microbiota capacity, and its alteration in dysregulated states, would enable the prediction individual responses to drugs toxins improve safety assessment. Here, we profiled chemical activities rat ex vivo, quantified capacity changes induced by oral exposure eight dietary compounds: tobramycin, colistin, acesulfame...
A crucial component of a substance registration and regulation is the evaluation human prenatal developmental toxicity. Current toxicological tests are based on mammalian models, but these costly, time consuming may pose ethical concerns. The zebrafish embryo has evolved as promising alternative model to study However, implementation embryotoxicity test challenged by lacking information relevance observed morphological alterations in fish for Elucidating mechanism toxicity could help...
Abstract Angiogenesis is a key process in embryonic development, disruption of this can lead to severe developmental defects, such as limb malformations. The identification molecular perturbations representative antiangiogenesis zebrafish embryo (ZFE) may guide the assessment toxicity from an endpoint- mechanism-based approach, thereby improving extrapolation findings humans. Thus, aim study was discover changes characteristic and toxicity. We exposed ZFEs two antiangiogenic drugs (SU4312,...
The diversity of microbial species in the gut has a strong influence on health and development host. Further, there are indications that variation expression bacterial metabolic enzymes is less diverse than taxonomic profile, underlying importance microbiome functionality, particularly from toxicological perspective. To address these relationships, composition Wistar rats was altered by 28 day oral treatment with antibiotics tobramycin or colistin sulfate. On basis 16S marker gene sequencing...
Cell-based metabolomics provides multiparametric physiologically relevant readouts that can be highly advantageous for improved, biologically based decision making in early stages of compound development. Here, we present the development a 96-well plate LC-MS/MS-based targeted screening platform classification liver toxicity modes action (MoAs) HepG2 cells. Different parameters workflow (cell seeding density, passage number, cytotoxicity testing, sample preparation, metabolite extraction,...
An understanding of the changes in gut microbiome composition and its associated metabolic functions is important to assess potential implications thereof on host health. Thus, elucidate connection between fecal plasma metabolomes, two poorly bioavailable carbapenem antibiotics (doripenem meropenem), were administered a 28-day oral study male female Wistar rats. Additionally, recovery metabolomes doripenem-exposed rats studied one weeks after antibiotic treatment (i.e., doripenem-recovery...
Abstract Omics techniques have been increasingly recognized as promising tools for Next Generation Risk Assessment. Targeted metabolomics offer the advantage of providing readily interpretable mechanistic information about perturbed biological pathways. In this study, a high-throughput LC–MS/MS-based broad targeted system was applied to study nitrofurantoin metabolic dynamics over time and concentration provide mechanistic-anchored approach point departure (PoD) derivation. Upon exposure at...
Cancer cell lines are widely used as in vitro models to study tumor biology and for efficacy testing of novel anti-cancer therapeutics. In the past, most this work has been done using established that have cultured decades. Extensive propagation shown lead acquisition multiple genetic epigenetic alterations lines, leading decreased heterogeneity lack initiating well multi-lineage differentiation capacity. Consequently, only insufficiently resemble characteristics tumors thus limited...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is a very aggressive disease with poor prognosis. Treatment gemcitabine, the FOLFIRINOX scheme or nab-paclitaxel offer only modest increase in overall survival. For number of other carcinomas, tumor subtypes have been uncovered that allow use targeted therapies. Although PDAC were described, this malignancy clinically still treated as single disease. We established patient-derived models representing full spectrum previously identified...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive disease with poor prognosis. Treatment gemcitabine, the FOLFIRINOX scheme or nab-paclitaxel offer only modest increase in overall survival. For number of other carcinomas, tumor subtypes have been uncovered that allow use targeted therapies. Although PDAC were described, this malignancy clinically still treated as single disease. We established patient-derived models representing full spectrum previously identified...
Abstract PDAC is a highly aggressive disease with dismal prognosis [1, 2]. Despite extensive research and the discovery of several drug candidates, little progress has been reported since approval gemcitabine erlotinib [1]. Moreover, recent trials targeted therapies have shown only limited or no benefit For number other carcinomas, tumor subclasses uncovered that allow use therapies. The mutational landscape complex heterogeneous, raising question whether also exist in [3]. Collisson et al....