A5101637113- Single-cell and spatial transcriptomics
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Pregnancy and preeclampsia studies
- RNA Research and Splicing
- Prenatal Screening and Diagnostics
- Birth, Development, and Health
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Gene Regulatory Network Analysis
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
BGI Research
2024
BGI Group (China)
2018-2022
University of Chinese Academy of Sciences
2021-2022
China National GeneBank
2018-2019
Abstract Placenta plays essential role in successful pregnancy, as the most important organ connecting and interplaying between mother fetus. However, cellular characteristics molecular interaction of cell populations within fetomaternal interface is still poorly understood. Here, we surveyed single-cell transcriptomic landscape human full-term placenta revealed heterogeneity cytotrophoblast (CTB) stromal (STR) with fetal/maternal origin consecutively localized from fetal section (FS),...
Abstract Background Investigating cell fate decision and subpopulation specification in the context of neural lineage is fundamental to understanding neurogenesis neurodegenerative diseases. The differentiation process neural-tube-like rosettes vitro representative tube structures, which are composed radially organized, columnar epithelial cells give rise functional cells. However, underlying regulatory network commitment during early remains elusive. Results In this study, we investigated...
Human umbilical cord-derived mesenchymal stem/stromal cells (UMSCs) demonstrate great therapeutic potential in regenerative medicine. The use of UMSCs for clinical applications requires high quantity and good quality usually by vitro expansion. However, the heterogeneity characteristics cultured cognate human cord tissue at single-cell resolution remain poorly defined. In this study, we created a transcriptome profile culture-expanded UMSCs. Based on inferred cell clusters trajectory...
Human mesenchymal stem cells (hMSCs) are widely used in clinical research because of their multipotential, immunomodulatory, and reparative properties. Previous studies determined that hMSC spheroids from a three-dimensional (3D) culture possess higher therapeutic efficacy than conventional hMSCs monolayer (2D) culture. To date, various 3D methods have been developed to form but most them medium containing fetal bovine serum (FBS), which is not suitable for further use. Here, we demonstrate...
Mesenchymal stem/stromal cells derived from placenta (PMSCs) are an attractive source for regenerative medicine because of their multidifferentiation potential and immunomodulatory capabilities. However, the cellular molecular heterogeneity PMSCs has not been fully characterized. Here, we applied single-cell RNA sequencing (scRNA-seq) assay transposase-accessible chromatin (scATAC-seq) techniques to cultured human full-term placenta. Based on inferred characteristics cell clusters, identify...
Abstract Human mesenchymal stem cells (hMSCs) are widely used in clinical research because of their multipotential, immunomodulatory, and reparative properties. Previous studies determined that hMSC spheroids from three-dimensional (3D) culture possess higher therapeutic efficacy than conventional hMSCs monolayer (2D) culture. To date, various 3D methods have been developed to form spheroids, but most them medium containing fetal bovine serum (FBS), which is not suitable for further use....
Placenta play essential role in successful pregnancy, as the most important organ connecting and interplaying between mother fetus. However, cellular molecular characteristics of fetal origin maternal cell populations within fetomaternal interface still is poorly understood. Here, we profiled transcriptomes single cells with well-defined maternal-fetal that consecutively localized from section (FS), middle (Mid_S) to (Mat_S) human full-term placenta. Then, initially identified heterogeneity...
Abstract Background Investigating cell fate decision and subpopulation specification in the context of neural lineage is fundamental to understanding neurogenesis neurodegenerative diseases. The differentiation process neural-tube-like rosettes vitro representative tube structures, which are composed radially organized, columnar epithelial cells give rise functional cells. However, underlying regulatory network commitment during early remains elusive. Results In this study, we investigated...