A5058659054- Cutaneous Melanoma Detection and Management
- Cancer Cells and Metastasis
- Melanoma and MAPK Pathways
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- CAR-T cell therapy research
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- HER2/EGFR in Cancer Research
- Advanced Biosensing Techniques and Applications
- Brain Metastases and Treatment
- Nonmelanoma Skin Cancer Studies
- Cell Image Analysis Techniques
- Cutaneous lymphoproliferative disorders research
- Sarcoma Diagnosis and Treatment
- Ocular Oncology and Treatments
- Angiogenesis and VEGF in Cancer
- vaccines and immunoinformatics approaches
- Tumors and Oncological Cases
- melanin and skin pigmentation
- Reconstructive Surgery and Microvascular Techniques
- Radiomics and Machine Learning in Medical Imaging
- Genomics and Chromatin Dynamics
- Retinal Diseases and Treatments
University Hospital of Zurich
2015-2024
University of Zurich
2012-2024
Massachusetts General Hospital
2024
Harvard University
2024
Max Planck Institute of Biochemistry
2023
Université Paris Sciences et Lettres
2021
Université Paris Cité
2021
Institut Curie
2021
Zentrum für Kinderheilkunde
2016
Innsbruck Medical University
2016
Abstract Human melanoma is composed of distinct cell types reminiscent neural crest derivatives and contains multipotent cells that express the stem markers CD271(p75NTR) Sox10. When isolated from solid tumors by using a method leaves intact surface epitopes, CD271-positive, but not CD271-negative, formed on transplantation into nude or nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. These fully mirrored heterogeneity parental could be passaged more than 5 times. In...
The function of the linea alba is to maintain abdominal muscles at a certain proximity each other. In case long-lasting increased intra-abdominal pressure, widens. Yet, as existence priori implicates physiological distance between two rectus muscles, question arises what normal width is. To evaluate alba, we examined 150 nulliparous women 20 and 45 years age with body mass index < 30 kg m(-2) by ultrasound three reference points: origin xiphoid 3 cm above 2 below umbilicus. examination...
Optimal vaccine strategies must be identified for improving T ‐cell vaccination against infectious and malignant diseases. Mel Q b G 10 is a virus‐like nano‐particle loaded with A ‐type C p ‐oligonucleotides ( ‐ ODN ) coupled to peptide 16–35 derived from M elan‐ / MART ‐1. In this phase IIa clinical study, four groups of stage III‐IV melanoma patients were vaccinated el 10, given (i) IFA ontanide) s.c.; (ii) s.c. topical I miquimod; (iii) i.d. or (iv) as intralymph node injection. total,...
Background: Clinical observations are suggesting accelerated granulation tissue formation in traumatic wounds treated with vacuum-assisted closure (VAC). Aim of this study was to determine the impact VAC therapy versus alternative Epigard application on local inflammation and neovascularization soft wounds. Methods: Thirty-two patients requiring temporary coverage (VAC n = 16; 16) were included. At each change dressing, samples wound fluid serum collected (n 80). The cytokines interleukin...
The epidermal growth factor receptor family member HER3 is overexpressed in diverse human cancers and has been associated with poor prognosis breast, lung, ovarian cancer. However, the relevance of regard to its prognostic significance function primary melanoma metastases remains largely elusive.HER3 protein expression was analyzed immunohistochemically using tissue microarrays 130 87 relative established clinical variables. possibility an influence on cell proliferation, migration,...
Melanoma is the most fatal skin cancer, but etiology of this devastating disease still poorly understood. Recently, transcription factor Sox10 has been shown to promote both melanoma initiation and progression. Reducing SOX10 expression levels in human cells a genetic mouse model, efficiently abolishes tumorigenesis by inducing cell cycle exit apoptosis. Here, we show that anti-tumorigenic effect functionally involves SOX9, related upregulated upon loss SOX10. Unlike SOX10, SOX9 not required...
Angiotropism/pericytic mimicry and vascular co-option involve tumor cell interactions with the abluminal surface. These two phenomena may be closely related. However, investigations of processes have developed in an independent fashion different explanations offered as to their biological nature. Angiotropism describes propensity cells spread distantly via continuous migration along surfaces, or extravascular migratory metastasis (EVMM). Vascular has been proposed alternative mechanism by...
Importance A standardized pathology classification system for melanocytic lesions is needed to aid both pathologists and clinicians in cataloging currently existing diverse terminologies the diagnosis treatment of patients. The Melanocytic Pathology Assessment Tool Hierarchy Diagnosis (MPATH-Dx) has been developed this purpose. Objective To revise MPATH-Dx version 1.0 tool, using feedback from dermatopathologists participating National Institutes Health–funded Reducing Errors Interpretations...
Stem cell-like cells have recently been identified in melanoma cell lines, but their relevance for pathogenesis is controversial. To characterize the stem signature of melanoma, expression markers BMI-1 and nestin was studied 64 cutaneous melanomas, 165 metastases as well 53 lines. renewal factor a transcriptional repressor Ink4a/Arf locus encoding p16(ink4a) p14(Arf). Increased nuclear detectable 41 (64%) primary 117 (71%) 15 (28%) High observed 14 56 melanomas (25%), 84 (50%) 21 lines...
// Marieke I. G. Raaijmakers 1 , Daniel S. Widmer Apurva Narechania 2 Ossia Eichhoff Sandra N. Freiberger 1, 4 Judith Wenzina Phil F. Cheng Daniela Mihic-Probst 3 Rob Desalle Reinhard Dummer Mitchell P. Levesque Department of Dermatology, University Zurich, Hospital Zürich, Switzerland American Museum Natural History, New York, USA Pathology, Zürich Skin and Endothelium Research Division, Medical Vienna, Austria Correspondence to: Levesque, email: Mitchell.Levesque@usz.ch Keywords: melanoma,...
<b><i>Introduction:</i></b> In December 2019, the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic broke out. The virus rapidly spread globally, resulting in a major world public-health crisis. disease manifestation occurs tract. However, further studies documented other systemic involvement. This study investigates histopathologic eye changes postmortem material of coronavirus 2019 (COVID-19) patients....
Immune checkpoint inhibitors are standard-of-care for the treatment of advanced melanoma, but their use is limited by immune-related adverse events. Proteomic analyses and multiplex cytokine chemokine assays from serum at baseline event onset indicated aberrant T cell activity with differential expression type I III immune signatures. This was in line finding an increase proportion CD4+ cells IL-17A peripheral blood using flow cytometry. Multiplex immunohistochemistry spatial transcriptomics...
Baseline staging in patients with primary cutaneous malignant melanoma (MM) is routine, but the diagnostic accuracy and impact on clinical outcome are still unclear.To evaluate sensitivity specificity of baseline early detection regional lymph node metastases or distant MM.One hundred consecutive MM Breslow's tumour thickness over 1.0 mm were enrolled. All had an extensive including physical examination, ultrasound (US) abdomen nodes, chest X-ray, whole-body positron emission tomography...
Abstract Dynamic polarisation of tumour cells is essential for metastasis. While the role during dedifferentiation and migration well established, metastasising phases detachment has not been investigated. Here we identify characterise a type maintained by single in liquid phase termed single-cell (sc) polarity investigate its We demonstrate that sc an inherent feature from different entities observed circulating patients. Functionally, propose pole directly involved early attachment,...
Drivers of Spitz neoplasms include activating point mutations in HRAS and Spitz-associated genomic fusions. It has become evident that some BRAF -mutated melanocytic can morphologically mimic tumors (STs). These have been termed mutated spitzoid (BAMS). In this study, 17 experts from the International Melanoma Pathology Study Group assessed 54 cases which included 40 BAMS 14 true STs. The participants reviewed blinded to data selected among several diagnostic options, including BAMS, ST,...
Lymph node (LN) status is an important prognostic factor in melanoma patients. p16 expression and proliferation rate (MIB-1) of primary melanomas have been suggested as a marker metastatic potential. In this study, the correlation with LN tumor-specific survival was investigated melanomas. MIB-1 were analyzed by immunohistochemistry 64 patients cutaneous melanoma. Thirty four nevi used control. All underwent sentinel lymph staging. Three different staining patterns observed: combination...
Recent molecular studies provide evidence for a significant transcriptional plasticity of tumor cell subpopulations that facilitate an active contribution to vasculature. This feature is accompanied by morphological changes both in vitro and vivo. Herein, we investigated the cells with special focus on vasculogenic mimicry neovascularisation human melanoma mouse xenografts lines. In xenograft experiments, different vessel markers green fluorescent protein expression were used show how...
Twenty per cent of sentinel lymph node (SLN)-positive melanoma patients have positive non-SLN nodes in completion dissection (CLND). We investigated SLN tumour load, non-sentinel positivity and disease-free survival (DFS) to assess whether certain could be spared CLND. Sentinel biopsy was performed on 392 between 1999 2005. Median observation period 38.8 months. load did not predict positivity: 30.8% with macrometastases (⩾2 mm) 16.4% micrometastases (⩽2 had (P=0.09). Tumour recurrences...