A5056191434- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Neuroendocrine Tumor Research Advances
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- Pancreatic function and diabetes
- Chromatin Remodeling and Cancer
- Cancer Research and Treatments
- Renal and related cancers
- Hedgehog Signaling Pathway Studies
- FOXO transcription factor regulation
- Microtubule and mitosis dynamics
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Angiogenesis and VEGF in Cancer
- Pluripotent Stem Cells Research
- Cancer, Hypoxia, and Metabolism
- Cancer, Stress, Anesthesia, and Immune Response
- Lung Cancer Research Studies
- Telomeres, Telomerase, and Senescence
- Chemical Reactions and Isotopes
- Chemokine receptors and signaling
- Immunotherapy and Immune Responses
- Mesenchymal stem cell research
- Gastric Cancer Management and Outcomes
Universität Ulm
2015-2024
University Hospital Ulm
2015-2024
Spanish National Cancer Research Centre
2009-2015
Clinical Research Organization
2012
Ludwig-Maximilians-Universität München
2007-2011
Nottingham Trent University
2010-2011
Helmholtz Zentrum München
2011
University of Nottingham
2010
Cancer Clinic
2010
LMU Klinikum
2007
Objective The generation of acinar and ductal cells from human pluripotent stem (PSCs) is a poorly studied process, although various diseases arise this compartment. Design We designed straightforward approach to direct PSCs towards pancreatic organoids resembling progeny. Results Extensive phenotyping the not only shows appropriate marker profile but also ultrastructural, global gene expression functional hallmarks pancreas in dish. Upon orthotopic transplantation into immunodeficient mice,...
Chronic pancreatitis and pancreatic ductal adenocarcinoma (PDAC) are characterized by extensive fibrosis. Importantly, in PDAC, this results poor vascularization impaired drug delivery to the cancer cells. Therefore, combined targeting of tumor stroma chemotherapy should enhance response rates, but negative outcome a recent phase III clinical trial for combination hedgehog pathway inhibition suggests that other means also need be considered. Emerging data indicate elimination stem cells as...
<h3>Objectives</h3> The tumour stroma/microenvironment not only provides structural support for development, but more importantly it cues to cancer stem cells (CSCs) that regulate their self-renewal and metastatic potential. This is certainly true pancreatic ductal adenocarcinomas (PDAC), where tumour-associated fibroblasts, stellate immune create an abundant paracrine niche CSCs via microenvironment-secreted factors. Thus understanding the role stroma play in PDAC development CSC biology of...
Abstract Pancreatic ductal adenocarcinoma (PDAC), the fourth leading cause of cancer death, has a 5-year survival rate approximately 7–9%. The ineffectiveness anti-PDAC therapies is believed to be due existence subpopulation tumor cells known as stem (CSCs), which are functionally plastic, and have exclusive tumorigenic, chemoresistant metastatic capacities. Herein, we describe 2D in vitro system for long-term enrichment pancreatic CSCs that amenable biological CSC-specific studies. By...
Abstract Pancreatic cancer stem cells (PaCSCs) drive pancreatic tumorigenesis, chemoresistance and metastasis. While eliminating this subpopulation of would theoretically result in tumor eradication, PaCSCs are extremely plastic can successfully adapt to targeted therapies. In study, we demonstrate that increase expression interferon-stimulated gene 15 (ISG15) protein ISGylation, which essential for maintaining their metabolic plasticity. CRISPR-mediated ISG15 genomic editing reduces overall...
Objective The lysyl oxidase-like protein 2 (LOXL2) contributes to tumour progression and metastasis in different entities, but its role pancreatic ductal adenocarcinoma (PDAC) has not been evaluated immunocompetent vivo PDAC models. Design Towards this end, we used patient data sets, patient-derived xenograft vitro models, four conditional genetically-engineered mouse models (GEMMS) dissect the of LOXL2 PDAC. For GEMM-based studies, K-Ras +/LSL-G12D ; Trp53 LSL-R172H Pdx1-Cre mice (KPC) (KC)...
Previous studies by our group have shown that oxidative phosphorylation (OXPHOS) is the main pathway which pancreatic cancer stem cells (CSCs) meet their energetic requirements; therefore, OXPHOS represents an Achille's heel of these highly tumorigenic cells. Unfortunately, therapies target in CSCs are lacking. The safety and anti-CSC activity a ruthenium complex featuring bipyridine terpyridine ligands one coordination labile position (Ru1) were evaluated across primary cultures vivo, using...
Cord blood-derived human endothelial colony-forming cells (ECFCs) bear a high proliferative capacity and potently enhance tissue neovascularization in vivo. Here, we investigated whether the leading mechanism for functional improvement relates to their physical vascular incorporation or perivascular paracrine effects can be further enhanced by dual-cell-based therapy, including mesenchymal stem (MSCs).ECFCs MSCs were lentivirally transduced with thymidine kinase suicide gene driven...
Abstract The identification of novel approaches to specifically target the DNA-damage checkpoint response in chemotherapy-resistant cancer stem cells (CSC) solid tumors has recently attracted great interest. We show here colon cell lines and primary that inhibition checkpoint-modulating phosphoinositide 3-kinase-related (PIK) kinases preferentially depletes chemoresistant exclusively tumorigenic CD133+ fraction. observed a time- dose-dependent disproportionally pronounced loss consecutive...
Organotypic cultures derived from pancreatic ductal adenocarcinoma (PDAC) termed cancer organoids (PDOs) recapitulate the primary and can be or metastatic biopsies. Although isolation culture of patient-derived were established several years ago, pros cons for individualized medicine have not been comprehensively investigated to date.We conducted a feasibility study, systematically comparing head-to-head xenograft tumor (PDX) PDX-derived by rigorous immunohistochemical molecular...
(ATM) is the most frequently mutated DNA damage response gene, involved in homologous recombination (HR), pancreatic ductal adenocarcinoma (PDAC).
Objective— Vasculogenic progenitor cell therapy for ischemic diseases bears great potential but still requires further optimization justifying its clinical application. Here, we investigated the effects of in vivo tissue engineering by combining vasculogenic progenitors with injectable scaffolds releasing controlled amounts proangiogenic growth factors. Methods and Results— We produced biodegradable, polylactic coglycolic acid–based single factors or combinations vascular endothelial factor,...
Stem and progenitor cell therapy is a novel strategy to enhance cardiovascular regeneration. Cell isolation procedures are crucial for the functional activity of administered cellular product. Therefore, new techniques have be evaluated in comparison Ficoll procedure as current gold standard. Here we prospectively point-of-care device (Harvest BMAC System) concentration bone marrow total nucleated cells (TNC) mononucleated (MNC). The yield numbers TNC was 2.4-fold higher Harvest compared...