A5040773671- Microbial metabolism and enzyme function
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- FOXO transcription factor regulation
- Dermatological and Skeletal Disorders
- Cancer Cells and Metastasis
- Muscle Physiology and Disorders
- CRISPR and Genetic Engineering
- Macrophage Migration Inhibitory Factor
- Pediatric Urology and Nephrology Studies
- Cardiac, Anesthesia and Surgical Outcomes
- Nuclear Structure and Function
- Vascular anomalies and interventions
- Spatial Neglect and Hemispheric Dysfunction
- PI3K/AKT/mTOR signaling in cancer
- Kruppel-like factors research
- Motor Control and Adaptation
- Histone Deacetylase Inhibitors Research
- Porphyrin Metabolism and Disorders
- Advanced Breast Cancer Therapies
- Cancer Diagnosis and Treatment
Instituto de Salud Carlos III
2012-2025
Instituto de Investigación de Enfermedades Raras
2020-2025
Weatherford College
2023
Instituto de Investigaciones Biomédicas Sols-Morreale
2011-2022
Universidad Autónoma de Madrid
2011-2022
Centro de Investigación Biomédica en Red de Cáncer
2017-2019
Spanish National Cancer Research Centre
2003-2014
Centro Nacional de Epidemiología
2003-2012
Hôpital Avicenne
2011
Unidades Centrales Científico-Técnicas
2010
Basal‐like breast carcinoma is characterized by the expression of basal/myoepithelial markers, undifferentiated phenotype, highly aggressive behaviour and frequent triple negative status (ESR−, PR−, Her2neu−). We have previously shown that epithelial–mesenchymal transition (EMT) occurs in basal‐like tumours identified Lysyl‐oxidase‐like 2 (LOXL2) as an EMT player poor prognosis marker squamous cell carcinomas. now show LOXL2 mRNA overexpressed human Breast lines with phenotype a specific...
The lysyl oxidase-like protein LOXL2 has been suggested to contribute tumor progression and metastasis, but in vivo evidence lacking. Here we provide functional that is a key driver of breast cancer metastasis two conditional transgenic mouse models PyMT-induced cancer. ablation mammary cells dramatically decreased lung whereas overexpression promoted metastatic growth. depletion or does not affect extracellular matrix stiffness organization primary tumors, implying function for independent...
Objective The lysyl oxidase-like protein 2 (LOXL2) contributes to tumour progression and metastasis in different entities, but its role pancreatic ductal adenocarcinoma (PDAC) has not been evaluated immunocompetent vivo PDAC models. Design Towards this end, we used patient data sets, patient-derived xenograft vitro models, four conditional genetically-engineered mouse models (GEMMS) dissect the of LOXL2 PDAC. For GEMM-based studies, K-Ras +/LSL-G12D ; Trp53 LSL-R172H Pdx1-Cre mice (KPC) (KC)...
Forkhead box L2 ( FOXL2 ) encodes a transcription factor essential for sex determination, and ovary development maintenance. Mutations in this gene are implicated syndromes involving premature ovarian failure granulosa cell tumors (GCTs). This rare cancer accounts less than 5% of diagnosed cancers is causally associated with the c.402C>G, p.C134W mutation 97% adult cases (AGCTs). In study, we employed CRISPR technology to specifically eliminate c.402C>G tumor cells. Our results show...
The Cip/Kip family, namely, p21Cip1, p27Kip1, and p57Kip2, are stoichiometric cyclin-dependent kinase inhibitors (CKIs). Paradoxically, they have been proposed to also act as positive regulators of Cdk4/6-cyclin D by stabilizing these heterodimers. Loss p21Cip1 p27Kip1 reduces complexes, although with limited phenotypic consequences compared the embryonic lethality Cdk4/6 or triple cyclin deficiency. This milder phenotype was attributed Cdk2 compensatory mechanisms. To address this...
Most human tumors harbor mutations that misregulate the early phases of cell cycle. Here, we summarize genetic evidence, mostly obtained in our laboratory using strains gene-targeted mice, provides direct experimental support for a role Cdk4 tumor development. Moreover, these studies challenge some well-established concepts regarding Cdks during For instance, they have illustrated and Cdk6 are not essential division embryonic development except hematopoietic system. More surprisingly, mice...
Background: The goal of this study was to determine if adenovirus-delivered LOXL2 protects against progressive knee osteoarthritis (OA), assess its specific mechanism action; and the overexpression in transgenic mice can protect development OA-related cartilage damage joint disability. Methods: Four-month-old Cho/+ male female were intraperitoneally injected with either Adv-RFP-LOXL2 or an empty vector twice a month for four months. proteoglycan levels expression anabolic catabolic genes...
Amyloid-β 40 peptides [Aβ1-40 (Aβ40)] are present within amyloid plaques in the brains of patients with Alzheimer's disease (AD). Even though Aβ considered neurotoxic, they can mediate many biological processes, both adult and throughout brain development. However, physiological function these remains poorly understood, existing data sometimes controversial. Here, we analyze compare effects monomeric Aβ40 on biology differentiating human neural stem cells (human NSCs). For that purpose, used...
Lysyl oxidase-like 2 (LOXL2) is a copper-dependent monoamine oxidase that contributes to the remodelling of extracellular matrix (ECM) by cross linkage collagen and elastin fibres has emerged as potential therapeutic target in cancer fibrosis. In skin, LOXL2 essential for epidermal cell polarity differentiation. However, its role dermis not been evaluated. We found Loxl2 dispensable mouse dermal development, maturation homeostasis, yet affects stiffness. Neither loss nor increased expression...
Laminopathies are causally associated with mutations on the Lamin A/C gene (LMNA). To date, more than 400 in LMNA have been reported patients. These widely distributed throughout entire and a wide range of phenotypes. Unfortunately, little is known about mechanisms underlying effect majority these mutations. This case 40 that located at exon 4. Using CRISPR/Cas9 technology, we generated collection Lmna 4 mutants mouse C2C12 myoblasts. cell models included different types deletions presence...
Laminopathies are causally associated with mutations on Lamin A gene (LMNA). To date, more than 400 in LMNA have been reported patients. These widely distributed throughout the entire and a wide range of phenotypes. Unfortunately, little is known about mechanisms underlying effect majority these mutations. This case 40 that located at exon 4. Using CRISPR/Cas9 technology, we generated collection Lmna 4 mutants mouse C2C12 myoblasts. cell models include different types deletions presence...
Abstract FOXL2 is a transcription factor essential for sex determination and ovary development maintenance. Mutations in this gene are implicated syndromes involving premature ovarian failure granulosa cell tumors (GCTs). This rare cancer accounts less than 5% of diagnosed cancers causally associated with the c.402C>G, p.C134W mutation 97% adult cases (AGCTs). In study, we employed CRISPR technology to specifically eliminate c.402C>G tumor cells. Our results show that Cas9-mediated...