A5020653832- Zebrafish Biomedical Research Applications
- Chromosomal and Genetic Variations
- Genetic diversity and population structure
- Animal Genetics and Reproduction
- Genetic and phenotypic traits in livestock
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Fish Ecology and Management Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Phylogenetic Studies
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Metal complexes synthesis and properties
- Aquaculture disease management and microbiota
- Reproductive biology and impacts on aquatic species
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Cervical Cancer and HPV Research
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
Universidade de Santiago de Compostela
2016-2025
Instituto de Investigación Sanitaria de Santiago
2021-2025
Université de Montréal
2021-2025
University of Vienna
2024
Universidade de Vigo
2024
Park Terrace Care Center
2023
Leiden University
2022
Institut Català d'Oncologia
2021
Instituto Maimónides de Investigación Biomédica de Córdoba
2021
University of Verona
2021
Abstract Background Gene expression analysis by reverse transcription quantitative PCR (qPCR) is the most widely used method for analyzing of a moderate number genes and also validation microarray results. Several issues are crucial successful qPCR study, particularly selection internal reference normalization efficiency determination. There no agreement on which best to detect stable neither how perform In this study we offer comprehensive evaluation characteristics gene methods decide one...
Abstract Cellular senescence is a stress response that limits the proliferation of damaged cells by establishing permanent cell cycle arrest. Different stimuli can trigger but excessive production or impaired clearance these lead to their accumulation during aging with deleterious effects. Despite this potential negative side senescence, its physiological role as pro‐regenerative and morphogenetic force has emerged recently after identification programmed embryogenesis wound healing limb...
Abstract Pancreatic ductal adenocarcinoma (PDAC), the fourth leading cause of cancer death, has a 5-year survival rate approximately 7–9%. The ineffectiveness anti-PDAC therapies is believed to be due existence subpopulation tumor cells known as stem (CSCs), which are functionally plastic, and have exclusive tumorigenic, chemoresistant metastatic capacities. Herein, we describe 2D in vitro system for long-term enrichment pancreatic CSCs that amenable biological CSC-specific studies. By...
Previous studies by our group have shown that oxidative phosphorylation (OXPHOS) is the main pathway which pancreatic cancer stem cells (CSCs) meet their energetic requirements; therefore, OXPHOS represents an Achille's heel of these highly tumorigenic cells. Unfortunately, therapies target in CSCs are lacking. The safety and anti-CSC activity a ruthenium complex featuring bipyridine terpyridine ligands one coordination labile position (Ru1) were evaluated across primary cultures vivo, using...
Due to their extensive use, the release of zinc oxide nanoparticles (ZnO NP) into environment is increasing and may lead unintended risk both human health ecosystems. Access ZnO NP brain has been demonstrated, so potential toxicity on nervous system a matter particular concern. Although evaluation reported in several previous studies, specific effects are not completely understood and, particularly, genetic material organism behaviour poorly addressed. We evaluated toxic vitro vivo, role ions (Zn
Abstract Sex determination in fish is a labile character evolutionary terms. The sex-determining (SD) master gene can differ even between closely related species. This group an interesting model for studying the evolution of SD region and gonadal differentiation pathway. turbot (Scophthalmus maximus) flatfish great commercial value, where strong sexual dimorphism exists growth rate. Following QTL marker association approach five families natural population, we identified main at proximal end...
Abstract Cellular senescence is often considered a protection mechanism triggered by conditions that impose cellular stress. Continuous proliferation, DNA damaging agents or activated oncogenes are well-known activators of cell senescence. Apart from characteristic stable cycle arrest, this response also involves proinflammatory phenotype known as senescence-associated secretory (SASP). This, together with the widely interference pathways some oncoviruses, had led to hypothesis may be part...
Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest tumors, partly due to its intrinsic aggressiveness, metastatic potential, and chemoresistance contained cancer stem cells (CSCs). CSCs strongly rely on mitochondrial metabolism maintain their stemness, therefore representing a putative target for elimination. Since homeostasis depends tightly controlled balance between fusion fission processes, namely dynamics, we aim study this mechanism in context stemness. In human PDAC tissues, gene
The aim of this work was to develop niosomes for the ocular delivery epalrestat, a drug that inhibits polyol pathway and protects diabetic eyes from damage linked sorbitol production accumulation. Cationic were made using polysorbate 60, cholesterol, 1,2-di-O-octadecenyl-3-trimethylammonium propane. characterized dynamic light scattering, zeta-potential, transmission electron microscopy determine their size (80 nm; polydispersity index 0.3 0.5), charge (−23 +40 mV), shape (spherical)....
Abstract A consensus microsatellite-based linkage map of the turbot (Scophthalmus maximus) was constructed from two unrelated families. The mapping panel derived a gynogenetic family 96 haploid embryos and biparental diploid 85 full-sib progeny with known phase. total 242 microsatellites were mapped in 26 groups, six markers remaining unlinked. length 1343.2 cM, an average distance between 6.5 ± 0.5 cM. Similar female male maps evidenced. However, mean recombination at common intervals...
Zebrafish (Danio rerio) is a model organism that has emerged as tool for cancer research, being the second most common cause of death after cardiovascular disease humans in developed world. useful xenotransplantation human cells and toxicity studies different chemotherapeutic compounds vivo. Compared to murine model, zebrafish faster, can be screened using high-throughput methods lower maintenance cost, making it possible affordable create personalized therapies. While several cell...
Abstract Zebrafish has become a popular research model in the last years, and several diseases affecting zebrafish facilities have been reported. However, only one case of naturally occurring viral infections was described for this species. In 2015, infectious spleen kidney necrosis virus ( ISKNV ) detected from facility Spain. Affected fish showed lethargy, loss appetite, abnormal swimming, distention coelomic cavity and, most severe cases, respiratory distress, pale gills petechial...
The limitations of platinum complexes in cancer treatment have motivated the extensive investigation into other metal such as ruthenium. We herein present synthesis and characterization a new family ruthenium compounds 1a-5a with general formula [Ru(bipy)2L][CF3SO3]2 (bipy = 2,2'-bipyridine; L bidentate ligand: N,N; N,P; P,P; P,As) which been characterized by elemental analysis, ES-MS, 1H 31P-{1H} NMR, FTIR conductivity measurements. molecular structures four Ru(ii) were determined single...
Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal cancer, requiring novel treatments to target both cancer cells and stem (CSCs). Altered splicing emerging as hallmark an attractive therapeutic target. The core factor SF3B1 heavily altered in can be inhibited by Pladienolide-B, but its actionability PDAC unknown. We explored the presence role of interrogated potential actionable target.SF3B1 was analyzed tissues, RNA-seq dataset, publicly available databases, examining associations...
Pancreatic ductal adenocarcinoma (PDAC) has limited therapeutic options, particularly with immune checkpoint inhibitors. Highly chemoresistant 'stem-like' cells, known as cancer stem cells (CSCs), are implicated in PDAC aggressiveness. Thus, comprehending how this subset of evades the system is crucial for advancing novel therapies.
Forkhead box L2 ( FOXL2 ) encodes a transcription factor essential for sex determination, and ovary development maintenance. Mutations in this gene are implicated syndromes involving premature ovarian failure granulosa cell tumors (GCTs). This rare cancer accounts less than 5% of diagnosed cancers is causally associated with the c.402C>G, p.C134W mutation 97% adult cases (AGCTs). In study, we employed CRISPR technology to specifically eliminate c.402C>G tumor cells. Our results show...
Protocol for staining iPSC-derived neurons lipid peroxidation measurements using Live/Dead and C11-Bodipy 581/591 dyes, via flow cytometry
Halide perovskites have emerged at the forefront of semiconductor materials for photovoltaic and light-emitting devices. However, long-term stability toxicity are major barriers to their commercialization. In particular, lead (Pb) its environmental impact prompted exploration lead-free alternatives like tin (Sn) bismuth (Bi). Herein, a cytotoxicity study Pb-based perovskite (CsPbBr3 CsPbI3) Cs2AgBiBr6 nanocrystals in lung liver cell lines blood cells reported, revealing that both Pb Bi...
The first examples of Ru(II) h6-arene (benzene and p-cymene) complexes containing a bidentate triazolylidene-triazolide ligand have been prepared fully characterized. Their antiproliferative effect has investigated against tumour cells A2780 (ovarian carcinoma), HCT116 (colorectal HCT116dox carcinoma resistant to doxorubicin), in human dermal fibroblasts. Ru complex bearing the p-cymene arene group exhibited stronger across all tested cell lines, while benzene-containing displayed higher...
Background/Objectives: Metabolomic studies of different parasite-derived biomolecules, such as lipids, are needed to broaden the discovery novel targets and overcome anthelmintic resistance. Lipids involved in diverse functions biological systems, including parasitic helminths, but little is known about their role biology these organisms impact on host–parasite interactions. This study aimed characterize lipid profile secreted by Haemonchus contortus, major nematodes farm ruminants. Methods:...
CRISPR/Cas9 (clustered regulatory interspaced short palindromic repeats/CRISPR-associated 9) has revolutionized gene editing technology by offering high precision and efficiency. Despite its powerful potential, significant challenges remain to completely avoid side effects improve delivery systems. This review focuses on system cargoes the different types of vehicles. Emphasis is placed advantages, disadvantages efficiency each method as well introducing vehicles such lipid nanoparticles,...