A5080569326- Zebrafish Biomedical Research Applications
- Neurogenesis and neuroplasticity mechanisms
- Neuropeptides and Animal Physiology
- Nerve injury and regeneration
- Neuroscience and Neuropharmacology Research
- Neuroendocrine regulation and behavior
- Spinal Cord Injury Research
- Retinal Development and Disorders
- Neurobiology and Insect Physiology Research
- Receptor Mechanisms and Signaling
- Developmental Biology and Gene Regulation
- Congenital heart defects research
- Ichthyology and Marine Biology
- Aquatic life and conservation
- Neuroinflammation and Neurodegeneration Mechanisms
- Axon Guidance and Neuronal Signaling
- Photoreceptor and optogenetics research
- Regulation of Appetite and Obesity
- Nicotinic Acetylcholine Receptors Study
- MicroRNA in disease regulation
- Stress Responses and Cortisol
- Echinoderm biology and ecology
- Mitochondrial Function and Pathology
- Single-cell and spatial transcriptomics
- Hypothalamic control of reproductive hormones
Universidade de Santiago de Compostela
2016-2025
Centro de Investigaciones Biológicas Margarita Salas
2022
Cibus (United States)
2021
Queen Mary University of London
2018-2021
University of Edinburgh
2011-2016
Instituto Nacional de Psiquiatría
1996-2014
Temple University
2010-2012
Temple University Hospital
2012
University of California, Los Angeles
1994-1998
West Los Angeles College
1996
Opiate alkaloids are potent analgesics that exert multiple pharmacological effects in the nervous system by activating G protein-coupled receptors. Receptor internalization upon stimulation may be important for desensitization and resensitization, which affect cellular responsiveness to ligands. Here, we investigated agonist-induced of mu opioid receptor (MOR) vivo using guinea pig ileum as a model immunohistochemistry with an affinity-purified antibody C terminus rat MOR. Antibody...
In adult zebrafish, relatively quiescent progenitor cells show lesion-induced generation of motor neurons. Developmental neuron from the spinal domain (pMN) sharply declines already at 48 hours post-fertilisation (hpf). After that, mostly oligodendrocytes are generated same domain. We demonstrate here that within after a lesion or specific genetic ablation neurons 72 hpf, pMN reverts to expense oligodendrogenesis. contrast, dorsal Pax2-positive interneurons was not altered. Larval...
Abstract Cellular senescence is a stress response that limits the proliferation of damaged cells by establishing permanent cell cycle arrest. Different stimuli can trigger but excessive production or impaired clearance these lead to their accumulation during aging with deleterious effects. Despite this potential negative side senescence, its physiological role as pro‐regenerative and morphogenetic force has emerged recently after identification programmed embryogenesis wound healing limb...
Abstract The vertebrate brain emerged more than ~500 million years ago in common evolutionary ancestors. To systematically trace its cellular and molecular origins, we established a spatially resolved cell type atlas of the entire sea lamprey—a jawless species whose phylogenetic position affords reconstruction ancestral traits—based on extensive single-cell RNA-seq situ sequencing data. Comparisons this to neural data from mouse other jawed vertebrates unveiled various shared features that...
Abstract Monoaminergic innervation of the spinal cord has important modulatory functions for locomotion. Here we performed a quantitative study to determine plastic changes tyrosine hydroxylase‐positive (TH1 + ; mainly dopaminergic), and serotonergic (5‐HT ) terminals cells during successful regeneration in adult zebrafish. TH1 is derived from brain. After transection, immunoreactivity completely lost caudal cord. Terminal varicosities increase density rostral lesion site compared with...
Spinal muscular atrophy (SMA) is characterized by the selective loss of spinal motor neurons owing to reduced levels survival neuron (Smn) protein. In addition its well-established role in assembling constituents spliceosome, diverse cellular functions have been proposed for Smn, but reason why low this widely expressed protein result pathology still debated. longitudinal studies exon-level changes SMA mouse model tissues, designed determine contribution splicing dysfunction disease, we...
Sulfakinin (SK)/cholecystokinin (CCK)-type neuropeptides regulate feeding and digestion in protostomes (e.g. insects) chordates. Here, we characterised SK/CCK-type signalling for the first time a non-chordate deuterostome – starfish Asterias rubens (phylum Echinodermata). In this species, two (ArSK/CCK1, ArSK/CCK2) derived from precursor protein ArSK/CCKP act as ligands an receptor (ArSK/CCKR) these peptides/proteins are expressed nervous system, digestive tube feet, body wall. Furthermore,...
The poor regenerative capacity of descending neurons is one the main causes lack recovery after spinal cord injury (SCI). Thus, it crucial importance to find ways promote axonal regeneration. In addition, prevention retrograde degeneration leading atrophy/death an obvious prerequisite activate Lampreys show amazing SCI. Recent histological work in lampreys suggested that GABA, which massively released a SCI, could survival neurons. Here, we aimed study if acting through GABAB receptors,...
Ocular health may strongly benefit from the supply of antioxidant agents that counteract free radicals and reactive oxygen species responsible for long-term eye diseases. Additionally, natural antioxidants like resveratrol can inhibit bacteria growth restore microbiota. However, their use is hindered by limited solubility, fast degradation, low ocular permeability. This work aimed to overcome these limitations preparing single mixed micelles Pluronic® F127 casein serve as nanocarriers....
Background and Purpose Spinal cord injury (SCI) is a neurological condition that affects motor sensory functions below the site. The consequences of SCI are devastating for patients, although significant efforts have been done in last years, there no effective therapy. Baclofen has emerged few years as an interesting drug field. Already used clinical setting to control spasticity, baclofen shown important impact on recovery animal models, such lampreys mice. Experimental Approach Key Results...
By their phylogenetic position and marked epithalamic asymmetries, lampreys are relevant models for understanding the formation evolution of this trait across vertebrates. In study, we use a transcriptomic approach to identify novel signature markers characterize highly asymmetric, bipartite organization habenulae in lampreys. Lamprey subdivided into two complementary subdomains related, respectively, lateral/ventral medial/dorsal jawed vertebrates: dorsal, right-restricted subdomain...
Zebrafish (Danio rerio) has become a pivotal vertebrate model in biomedical research, renowned for its genetic similarity to humans, optical transparency, rapid embryonic development, and amenability experimental manipulation. In recent years, the derivation of cell lines from zebrafish embryos unlocked new possibilities vitro studies across developmental biology, toxicology, disease modeling, engineering. These embryo-derived cultures offer scalable, reproducible, ethically favorable...
Neuropeptides are diverse and evolutionarily ancient regulators of physiological/behavioural processes in animals. Here we have investigated the evolution comparative physiology luqin-type neuropeptide signalling, which has been characterised previously protostomian invertebrates. Phylogenetic analysis indicates that receptors tachykinin-type paralogous probably originated a common ancestor Bilateria. In deuterostomian lineage, signalling lost chordates but interestingly it retained...
The vertebrate brain emerged more than ∼500 million years ago in common evolutionary ancestors. To systematically trace its cellular and molecular origins, we established a spatially resolved cell type atlas of the entire sea lamprey – jawless species whose phylogenetic position affords reconstruction ancestral traits based on extensive single-cell RNA-seq situ sequencing data. Comparisons this to neural data from mouse other jawed vertebrates unveiled various shared features that enabled...
Abstract Neuroactive substances such as serotonin and other monoamines have been suggested to be involved in the transmission of gustatory signals from taste bud cells afferent fibers. Lampreys are earliest vertebrates that possess buds, although these differ structure buds jawed vertebrates, their neurochemistry remains unknown. We used immunofluorescence methods with antibodies raised against serotonin, tyrosine hydroxylase (TH), γ‐aminobutyric acid (GABA), glutamate, calcitonin...
Apoptosis is a major feature in neural development and important traumatic diseases. The presence of active caspases widely accepted marker apoptosis. We report here the method to study neuronal apoptotic death whole-mounted brain preparations using fluorochrome-labeled inhibitors (FLICA). As model we used axotomy-induced retrograde CNS larval sea lampreys. Once inside cell, FLICA reagents bind covalently causing cells fluoresce, whereas nonapoptotic remain unstained. fluorescent probe, poly...
Despite the importance of glutamate as a major excitatory neurotransmitter in brain, distribution glutamatergic populations brain most vertebrates is still unknown. Here, we studied for first time neurons forebrain sea lamprey (Petromyzon marinus), belonging to ancient group (agnathans). For this, used situ hybridization with probes vesicular transporter (VGLUT) larvae and immunofluorescence antiglutamate antibodies both adults. We also compared γ-aminobutyric acid (GABA) immunoreactivities...