A5034190349- Ocular Surface and Contact Lens
- Glaucoma and retinal disorders
- Circadian rhythm and melatonin
- Retinal Development and Disorders
- Corneal Surgery and Treatments
- Advanced Drug Delivery Systems
- Photoreceptor and optogenetics research
- Retinal Diseases and Treatments
- Fibroblast Growth Factor Research
- Nitric Oxide and Endothelin Effects
- Corneal surgery and disorders
- Connective tissue disorders research
- Proteoglycans and glycosaminoglycans research
- Neurological Disorders and Treatments
- Legionella and Acanthamoeba research
- Adenosine and Purinergic Signaling
- Advanced Glycation End Products research
- Ion channel regulation and function
- Nerve injury and regeneration
- Ion Transport and Channel Regulation
- Ophthalmology and Eye Disorders
- Food Allergy and Anaphylaxis Research
- Bone Metabolism and Diseases
- Technology in Education and Healthcare
- Neurobiology and Insect Physiology Research
Universidad Complutense de Madrid
2013-2024
Weatherford College
2012-2014
Hospital Clínico San Carlos
2005
Design of advanced contact lenses (CLs) demands materials that are safe and comfortable for the wearers preserve normal eye microbiota, avoiding chronic inflammation biofilm development. This work aimed to combine natural antibiofouling phosphorylcholine antioxidant prebiotic resveratrol as integral components CLs may have additional performance preventing oxidative-stress related diseases. Different from previous uses 2-methacryloyloxyethyl (MPC) coating, we explored feasibility adding MPC...
Melatonin and its analog 5-MCA-NAT (5-methylcarboxyamino-<i>N</i>-acetyl tryptamine) are active compounds reducing intraocular pressure (IOP). This action is mediated through MT<sub>2</sub> the putative MT<sub>3</sub> melatonin receptor, producing a transient reduction of IOP that lasts for few hours has not yet been characterized. The use causing decrease in chloride efflux from rabbit nonpigmented epithelial cells (NPE), possibly explaining IOP. inhibited NPE release...
Ocular health may strongly benefit from the supply of antioxidant agents that counteract free radicals and reactive oxygen species responsible for long-term eye diseases. Additionally, natural antioxidants like resveratrol can inhibit bacteria growth restore microbiota. However, their use is hindered by limited solubility, fast degradation, low ocular permeability. This work aimed to overcome these limitations preparing single mixed micelles Pluronic® F127 casein serve as nanocarriers....
Glaucoma is a multifactorial neurodegenerative disease and the second leading cause of blindness. Detection clinically relevant biomarkers would aid better diagnoses monitoring during treatment. In glaucoma, protein composition aqueous humor (AH) for discovery biomarkers. This study analyzes AH concentrations putative in patients with primary open-angle glaucoma (POAG) compared to control group. Biomarkers were selected from known oxidative-stress inflammatory pathways. Osteopontin (OPN),...
Oral administration of cholesterol-lowering statins, HMG-CoA reductase inhibitors, is associated with beneficial effects on eye conditions. This work aims to design contact lenses (CLs) that can sustainedly deliver pravastatin and thus improve the ocular efficacy while avoiding systemic side statins. Bioinspired hydrogels were prepared monomers resemble hydrophobic (ethylene glycol phenyl ether methacrylate) amino (2-aminoethyl methacrylamide hydrochloride) functionalities active site...
Melatonin analogs topically administered evoke a potent tear secretagogue effect in rabbits. This route of drug administration requires high concentration and frequent dosing due to its reduced ocular surface retention. Therefore, contact lenses (CLs) have emerged as an alternative drug-delivery system that prolongs retention the cornea, improving therapeutic performance. study explores vitro ability five commercially available hydrogel CLs act delivery for melatonin vivo analog-loaded CLs....
Melatonin, the MT<sub>2</sub> melatonin receptor agonist IIK7 [<i>N-butanoyl</i>-2-(2-methoxy-6<i>H</i>-isoindolo[2,1-<i>a</i>]indol-11-yl)ethanamine], and putative MT<sub>3</sub> 5-MCA-NAT [5-methoxycarbonylamino-<i>N</i>-acetyltryptamine] have previously been shown to reduce intraocular pressure (IOP) in ocular normotensive rabbits. To gain a better understanding of structure-activity relationship compounds that activate receptors mediating reductions IOP, novel analogs with rationally...
Melatonin is currently considered a promising drug for glaucoma treatment because of its ocular hypotensive and neuroprotective effects. We have investigated the effect melatonin analog 5-methoxycarbonylamino-<i>N</i>-acetyltryptamine, 5-MCA-NAT, on <i>β</i><sub>2</sub>/<i>α</i><sub>2A</sub>-adrenergic receptor mRNA as well protein expression in cultured rabbit nonpigmented ciliary epithelial cells. Quantitative polymerase chain reaction immunocytochemical assays revealed significant...
The purpose was to quantify ocular dopamine in rabbits after stimulation of the optic nerve head with short-wavelength (blue) light activate melanopsin expressed axons intrinsically photosensitive retinal ganglion cells (ipRGCs). Dopamine levels tears, aqueous humor, vitreous body, and retina (including choroid) were quantified blue 15 an optical fiber for 1 min, 10 or no (n = 5, each group). left eye all operated on introduce stimulate nerve, while contralateral served as internal control....
Abstract The first line of glaucoma treatment focuses on reducing intraocular pressure (IOP) through the prescription topical prostaglandin analogues, such as latanoprost (LAT). Topical ophthalmic medicines have low bioavailability due to their rapid elimination from ocular surface. Nanotechnology offers innovative ways enhancing antiglaucoma agents while administration frequency. This study aims combine LAT-loaded synthetic phosphatidylcholine liposomes with hyaluronic acid (0.2% w/v ) and...
Purpose . To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days original increasing concentration BAC from 0.1% to 0.2%. Materials and Methods An experimental, prospective, randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control ( n = 10) with 0.2% treatment 10). Saline solution (control) were instilled for consecutive days, twice daily. Tear...
We have previously demonstrated that melatonin and its analogue, 5-methoxycarbonylamino-N-acetyltryptamine (5-MCA-NAT), reduce intraocular pressure (IOP) in New Zealand rabbits. More recently, we shown 5-MCA-NAT can also regulate ciliary adrenoceptor gene expression. Like adrenoceptors, carbonic anhydrase (CA) enzymes are involved aqueous humour secretion by the ocular epithelium. Moreover, CA been reported to be regulated melatonin. Hence, aim of this study was investigate whether...
Melatonin has been shown to enhance tear secretion associated with dinucleotide diadenosine tetraphosphate. This study investigated the isolated action of melatonin and its analogs, agomelatine, <i>N</i>-butanoyl-2-(2-methoxy-6<i>H</i>-isoindolo[2,1-<i>a</i>]indol-11-yl) ethanamine (IIK7), 5-methoxycarbonylamino-<i>N</i>-cetyltryptamine (5-MCA-NAT) (10 <i>µ</i>l at 100 <i>µ</i>M), on when applied topically in rabbit cornea relationship MT1, MT2, MT3/quinone reductase QR2 receptors. The...
(1) Background: The purpose of this study was to synthesize melatonin-eluting contact lenses (CLs) and evaluate both the ocular kinetics released melatonin its effect on tear volume intraocular pressure. (2) Methods: In vitro, CLs were synthesized by using non-functionalized (HEMA) functionalized (HEMA/APMA) monomers. vivo, a short-term prospective randomized performed 15 rabbits divided into two groups: 12 wearing 3 without as control. levels in tears, aqueous humor, vitreous body retina,...
Introduction : Sarcopenia is defined as a loss of muscle mass and strength. ATP homeostasis crucial during myogenesis. We determined how the purinergic system modulates myogenesis using dipyridamole (blocks adenosine taken up by cells) tenofovir (inhibits release) in myoblast cell line. Methods: C2C12 cells were differentiated presence/absence tenofovir/dipyridamole, with/without A2B selective inhibitor PSB-603. Extra-/intracellular nucleotides examined via HPLC. The expression...
We have demonstrated that 5-methoxycarbonylamino-N-acetyltryptamine (5-MCA-NAT), reduces intraocular pressure (IOP) in rabbits. In addition, we reported a link between hypotensive effect of 5-MCA-NAT and sympathetic nervous system. Moreover, it is known aqueous humour production controlled by the activation adrenoceptors (ADRs) present ocular ciliary epithelium. Thus, aim this study to investigate if due regulation ADR genes expression. To confirm followed on rabbit IOP for 144 consecutive...
Contact lenses (CLs) have been suggested as drug delivery platforms capable of increasing the residence time on cornea and therefore its bioavailability. However, when targeting posterior segment eye, released from CLs still encounters barrier effect ocular tissues, which considerably reduces efficacy administration. This work aims at development able to simultaneously deliver an anti-inflammatory (dexamethasone sodium phosphate) a cell-penetrating peptide (penetratin), latter acting carrier...