A5051335844- Ocular Surface and Contact Lens
- Glaucoma and retinal disorders
- Advanced Drug Delivery Systems
- Corneal Surgery and Treatments
- Retinal Diseases and Treatments
- Circadian rhythm and melatonin
- Retinal Development and Disorders
- RNA Interference and Gene Delivery
- Corneal surgery and disorders
- Nanoparticle-Based Drug Delivery
- Proteoglycans and glycosaminoglycans research
- Nitric Oxide and Endothelin Effects
- Pharmaceutical Practices and Patient Outcomes
- Dendrimers and Hyperbranched Polymers
- Neurological Disorders and Treatments
- Advancements in Transdermal Drug Delivery
- Higher Education Teaching and Evaluation
- E-Learning and Knowledge Management
- Retinopathy of Prematurity Studies
- Rabbits: Nutrition, Reproduction, Health
- Educational Technology in Learning
- Retinal and Macular Surgery
- Intraocular Surgery and Lenses
- Ocular Disorders and Treatments
- Sleep and Wakefulness Research
Universidad Complutense de Madrid
2016-2025
Instituto de Investigación Sanitaria del Hospital Clínico San Carlos
2015-2025
Hospital Clínico San Carlos
2017-2025
Instituto de Salud Carlos III
2015-2023
UCB Pharma (Spain)
2011
Pharmaceutical Biotechnology (Czechia)
2010
The purpose of this research was to determine the potential use water-soluble anionic and cationic carbosilane dendrimers (generations 1–3) as mucoadhesive polymers in eyedrop formulations. Cationic decorated with ammonium –NH3+ groups were prepared by hydrosylilation Boc-protected allylamine followed deprotection HCl. Anionic terminal carboxylate also employed study. In vitro vivo tolerance studies performed human ocular epithelial cell lines rabbit eyes respectively. interaction...
The present study aims to develop a thermo-responsive-injectable hydrogel (HyG) based on PLGA-PEG-PLGA (PLGA = poly-(DL-lactic acid co-glycolic acid); PEG polyethylene glycol) deliver neuroprotective agents the retina over time. Two PLGA-PEG PLGA copolymers with different PEG:LA:GA ratios (1:1.54:23.1 and 1:2.25:22.5) for HyG-1 HyG-2 development respectively were synthetized characterized by techniques (gel permeation chromatography (GPC), nuclear magnetic resonance (NMR), dynamic light...
The increment in ocular drug bioavailability after topical administration is one of the main challenges pharmaceutical technology. For several years, different strategies based on nanotechnology, hydrogels or implants have been evaluated. Nowadays, tolerance ophthalmic preparations has become a critical issue and it essential to use well tolerated excipients. In present work, we explored potential gelatin nanoparticles (GNPs) loaded with timolol maleate (TM), beta-adrenergic blocker widely...
For the treatment of chronic ocular diseases such as glaucoma, continuous instillations eye drops are needed. However, frequent administrations hypotensive topical formulations can produce adverse surface effects due to active substance or other components formulation, preservatives excipients. Thus development unpreserved that well tolerated after is an important challenge improve ophthalmic therapies. Furthermore, several properties formulation in terms efficacy. In order achieve mentioned...
To develop an easy-to-perform combined model in human corneal epithelial cells (HCECs) and Balb/c mice macrophages J774.A1 (MP) for preliminary screening of potential ophthalmic therapeutic substances.HCECs were exposed to different osmolarities (350-500 mOsm/L) MTT assay was employed cell survival flow cytometry assess apoptosis-necrosis relative size (RCS) distribution. Effectiveness Betaine, L-Carnitine, Taurine at concentrations (ranging from 20 mM 200 mM) studied. Also, mucoadhesive...
This paper aims to develop smart hydrogels based on functionalized hyaluronic acid (HA) and PLGA-PEG-PLGA (PLGA,poly-(DL-lactic-co-glycolic acid); PEG,polyethylene glycol) for use as intraocular drug-delivery platforms. Anti-inflammatory agent dexamethasone-phosphate (0.2 %w/v) was the drug selected load hydrogels. Initially, different ratios of HA-aldehyde (HA-CHO) thiolated-HA (HA-SH) were assayed, selecting optimal concentrations 2 3 % (w/v), respectively. Optimized HA hydrogel...
To assess the in vitro tolerance and vivo efficacy of new unpreserved formulations timolol maleate (TM) aqueous solutions bioadhesive polymers used for dry eye treatment to compare them with three traditional TM formulations: Timabak (Thea, Madrid, Spain), benzalkonium chloride (BAK)-preserved Timoftol (Frosst Laboratories, BAK-preserved Timolol Sandoz Laboratories).New were composed (0.5%) carboxymethyl cellulose (0.5%), hyaluronic acid (0.2%), or hydroxypropylmethyl (0.3% 0.5%). In was...
Many diseases affecting the posterior segment of eye require repeated intravitreal injections with corticosteroids in chronic treatments. The periocular administration is a less invasive route attracting considerable attention for long-term therapies. In present work, dexamethasone-loaded poly(lactic-co-glycolic) acid (PLGA) microspheres (Dx-MS) were prepared using oil-in-water (O/W) emulsion solvent evaporation technique. MS characterized terms mean particle size and distribution, external...
This paper discusses the development and validation of a rapid method for reversed phase HPLC-UV quantification biodegradable poly(D,L-lactic-co-glycolic) acid (PLGA) microspheres co-loaded with two neuroprotective agents (dexamethasone melatonin) (DX-MEL-MSs) to be intravitreally administered as promising glaucoma treatment. The study was performed validate procedures that quantify content active substances entrapped into polymer matrix during an encapsulation efficiency assay amount drugs...
Glaucoma is a group of chronic irreversible neuropathies that affect the retina and optic nerve. It considered one leading causes blindness in world. Although it can be due to various causes, most important modifiable risk factor elevated intraocular pressure (IOP). In this case, treatment choice consists instilling antihypertensive formulations on ocular surface. The chronicity pathology, together with low bioavailability drugs are applied surface, make necessary instill very frequently,...
Abstract The first line of glaucoma treatment focuses on reducing intraocular pressure (IOP) through the prescription topical prostaglandin analogues, such as latanoprost (LAT). Topical ophthalmic medicines have low bioavailability due to their rapid elimination from ocular surface. Nanotechnology offers innovative ways enhancing antiglaucoma agents while administration frequency. This study aims combine LAT-loaded synthetic phosphatidylcholine liposomes with hyaluronic acid (0.2% w/v ) and...
The chronic use of hypotensive agents eventually leads to ocular surface damage and poor patient compliance during glaucoma management. Thus, new sustained drug delivery systems are needed. This work aimed develop osmoprotective latanoprost-loaded microemulsion formulations as potential treatments with protective properties. microemulsions were characterized latanoprost encapsulation efficacy determined. In-vitro tolerance, efficacy, cell internalization well cell-microemulsion interactions...