A5027012940- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Advanced Drug Delivery Systems
- Glaucoma and retinal disorders
- Corneal surgery and disorders
- RNA Interference and Gene Delivery
- Circadian rhythm and melatonin
- Proteoglycans and glycosaminoglycans research
- Dendrimers and Hyperbranched Polymers
- Biochemical effects in animals
- Nanoparticle-Based Drug Delivery
- Medicinal Plants and Bioactive Compounds
- Photodynamic Therapy Research Studies
- Safe Handling of Antineoplastic Drugs
- Antibiotics Pharmacokinetics and Efficacy
- Sleep and Wakefulness Research
- Advancements in Transdermal Drug Delivery
- Ophthalmology and Eye Disorders
- Stress Responses and Cortisol
- Polydiacetylene-based materials and applications
- Immunotherapy and Immune Responses
- Tryptophan and brain disorders
- Hydrogels: synthesis, properties, applications
- Cholinesterase and Neurodegenerative Diseases
- Ocular Infections and Treatments
Universidad Complutense de Madrid
2013-2025
Instituto de Investigación Sanitaria del Hospital Clínico San Carlos
2014-2025
Hospital Clínico San Carlos
2022-2025
Instituto de Salud Carlos III
2014-2023
UCB Pharma (Spain)
2011
To investigate the effect of topical insulin on epithelization in persistent epithelial defects (PED) refractory to usual treatment compared autologous serum.Retrospective, consecutive case-control series.The charts 61 patients with PED treated (case group) and 23 serum (control were reviewed. Primary efficacy end points percentage which was achieved, as well rate time until epithelization. Secondary point need for amniotic membrane transplantation (AMT) or other surgeries.Mean between...
Purpose.: Dry eye (DE) includes a group of diseases related to tear film disorders. Current trends for DE therapy focus on providing lipid components replace the damaged layer. Formulations that contain aqueous and mucin-like compounds may have additional therapeutic benefits patients. The aim this work was design evaluate novel formulations having potential become topical treatment DE. Methods.: Unpreserved liposomal composed phosphatidylcholine (PC), cholesterol, α-tocopherol (vit E) were...
The purpose of this research was to determine the potential use water-soluble anionic and cationic carbosilane dendrimers (generations 1–3) as mucoadhesive polymers in eyedrop formulations. Cationic decorated with ammonium –NH3+ groups were prepared by hydrosylilation Boc-protected allylamine followed deprotection HCl. Anionic terminal carboxylate also employed study. In vitro vivo tolerance studies performed human ocular epithelial cell lines rabbit eyes respectively. interaction...
Dry eye is commonly treated with artificial tears; however, developing tears similar to natural difficult due the complex nature of tears. We characterized and evaluated a novel tear formulation components lipid aqueous constituents Nano-liposomes, composed in part phosphatidylcholine, were dispersed an solution bioadhesive sodium hyaluronate. Liposome size, zeta potential, physicochemical properties fresh stored (4 °C) liposomal analyzed. In vitro tolerance was tested using human corneal...
For the treatment of chronic ocular diseases such as glaucoma, continuous instillations eye drops are needed. However, frequent administrations hypotensive topical formulations can produce adverse surface effects due to active substance or other components formulation, preservatives excipients. Thus development unpreserved that well tolerated after is an important challenge improve ophthalmic therapies. Furthermore, several properties formulation in terms efficacy. In order achieve mentioned...
The aim of this study is to assess if an adhesive biopolymer, sodium hyaluronate (NaHA), has synergistic effects with s-PRGF (a serum derived from plasma rich in growth factors and a blood derivative that already shown efficacy corneal epithelial wound healing), reduce time healing or posology. In vitro proliferation migration studies, both human (HCE) cells rabbit primary (RPCE) cultures, were carried out. addition, we performed studies vivo rabbits treated s-PRGF, NaHA, the combination...
This paper aims to develop smart hydrogels based on functionalized hyaluronic acid (HA) and PLGA-PEG-PLGA (PLGA,poly-(DL-lactic-co-glycolic acid); PEG,polyethylene glycol) for use as intraocular drug-delivery platforms. Anti-inflammatory agent dexamethasone-phosphate (0.2 %w/v) was the drug selected load hydrogels. Initially, different ratios of HA-aldehyde (HA-CHO) thiolated-HA (HA-SH) were assayed, selecting optimal concentrations 2 3 % (w/v), respectively. Optimized HA hydrogel...
Abstract The first line of glaucoma treatment focuses on reducing intraocular pressure (IOP) through the prescription topical prostaglandin analogues, such as latanoprost (LAT). Topical ophthalmic medicines have low bioavailability due to their rapid elimination from ocular surface. Nanotechnology offers innovative ways enhancing antiglaucoma agents while administration frequency. This study aims combine LAT-loaded synthetic phosphatidylcholine liposomes with hyaluronic acid (0.2% w/v ) and...
To assess the in vitro tolerance and vivo efficacy of new unpreserved formulations timolol maleate (TM) aqueous solutions bioadhesive polymers used for dry eye treatment to compare them with three traditional TM formulations: Timabak (Thea, Madrid, Spain), benzalkonium chloride (BAK)-preserved Timoftol (Frosst Laboratories, BAK-preserved Timolol Sandoz Laboratories).New were composed (0.5%) carboxymethyl cellulose (0.5%), hyaluronic acid (0.2%), or hydroxypropylmethyl (0.3% 0.5%). In was...
This paper discusses the development and validation of a rapid method for reversed phase HPLC-UV quantification biodegradable poly(D,L-lactic-co-glycolic) acid (PLGA) microspheres co-loaded with two neuroprotective agents (dexamethasone melatonin) (DX-MEL-MSs) to be intravitreally administered as promising glaucoma treatment. The study was performed validate procedures that quantify content active substances entrapped into polymer matrix during an encapsulation efficiency assay amount drugs...
Glaucoma is a group of chronic irreversible neuropathies that affect the retina and optic nerve. It considered one leading causes blindness in world. Although it can be due to various causes, most important modifiable risk factor elevated intraocular pressure (IOP). In this case, treatment choice consists instilling antihypertensive formulations on ocular surface. The chronicity pathology, together with low bioavailability drugs are applied surface, make necessary instill very frequently,...
The chronic use of hypotensive agents eventually leads to ocular surface damage and poor patient compliance during glaucoma management. Thus, new sustained drug delivery systems are needed. This work aimed develop osmoprotective latanoprost-loaded microemulsion formulations as potential treatments with protective properties. microemulsions were characterized latanoprost encapsulation efficacy determined. In-vitro tolerance, efficacy, cell internalization well cell-microemulsion interactions...
Compounded insulin eye drops were prepared at 1 IU/mL from commercially available subcutaneous by dilution in saline solution or artificial tears. Physicochemical characterization and vitro tolerance testing human conjunctival cells followed a 28-day short-term stability study under various conditions. The formulations isotonic (280–300 mOsm/L), had pH close to neutral (7–8), medium surface-tension values (<56 MN/m−1), low (≈1 mPa·s) (≈5 viscosities (compounded normal tear-based...
For the majority of cytotoxic drug preparations, such as bortezomib, unit dose information is not available. In addition, there a lack on physicochemical stability pharmaceutical preparation after opening; this crucial for its administration to patients in successive visits, and per-patient cost can be affected. The purpose our proposed study determine shelf life reconstituted liquid product under refrigeration clinical practice conditions. This evaluation was extended both vials...
Purpose: Anti‐inflammatory and antioxidant evaluation of idebenone (IDB) in ARPE‐19 cell line subsequent encapsulation PLGA (polylactic‐co‐glycolic‐acid)/vitamin E microspheres (MS), together with neurotrophic factors, to confer additional neuroprotective activity technological advantages. Methods: ARPE‐19, a human RPE line, were incubated for 24 IDB (0.5‐150 μM) determine viability by MTT assay. For anti‐inflammatory evaluation, cells pre‐treated (10 3 h then, stimulated LPS μg/mL;24 h)....
Aims/Purpose: The design of new topical ophthalmic nanosystems is linked to obtain non‐harmful carriers capable increasing drug bioavailability and reduce the frequency its instillations. Hypotensive actives such as latanoprost (L) are used decrease intraocular pressure (IOP) in glaucoma patients. Two novel formulations, L‐loaded liposomes (LL) microemulsions (LME), both including hyaluronic acid for mucoadhesive properties were elaborated. efficacy reducing IOP formulations (48 h) was...