A5073338263- Nerve injury and regeneration
- Mesenchymal stem cell research
- Spinal Cord Injury Research
- Parkinson's Disease Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Cerebral Palsy and Movement Disorders
- RNA Interference and Gene Delivery
- Neurogenesis and neuroplasticity mechanisms
- DNA Repair Mechanisms
- Botulinum Toxin and Related Neurological Disorders
- Muscle activation and electromyography studies
- Neurological Disease Mechanisms and Treatments
- Adipose Tissue and Metabolism
- Planarian Biology and Electrostimulation
- Neurotransmitter Receptor Influence on Behavior
- Electrospun Nanofibers in Biomedical Applications
- Lysosomal Storage Disorders Research
- Pluripotent Stem Cells Research
- Diamond and Carbon-based Materials Research
- Neuroscience and Neuropharmacology Research
- Muscle Physiology and Disorders
- Vagus Nerve Stimulation Research
- Alzheimer's disease research and treatments
- Complement system in diseases
- Nicotinic Acetylcholine Receptors Study
University of Minho
2020-2025
University of Beira Interior
2020
Polymer biomaterials are being considered for tissue regeneration due to the possibility of resembling different extracellular matrix characteristics. However, most current scaffolds cannot respond physical-chemical modifications cell microenvironment. Stimuli-responsive materials, such as electroactive smart polymers, increasingly gaining attention once they can produce electrical potentials without external power supplies. The presence piezoelectricity in human tissues like cartilage and...
Parkinson's disease (PD) is a prevalent movement disorder characterized by the progressive loss of dopaminergic neurons in substantia nigra pars compacta (SNpc). The 6-hydroxydopamine (6-OHDA) lesion still one most widely used techniques for modeling rodents. Despite commonly rats, it can be challenging to reproduce similar mice. Moreover, there lack characterization extent behavioral deficits and neuronal loss/neurotransmitter system unilateral mouse models. In this study, we present an...
Spinocerebellar ataxia type 3 (SCA3) is an adult-onset neurodegenerative disease caused by a polyglutamine expansion in the ataxin-3 (ATXN3) gene. No effective treatment available for this disorder, other than symptom-directed approaches. Bile acids have shown therapeutic efficacy models. Here, we pinpointed tauroursodeoxycholic acid (TUDCA) as efficient therapeutic, improving motor and neuropathological phenotype of SCA3 nematode mouse Surprisingly, transcriptomic functional vivo data...
Mesenchymal stem cell-based therapies have been studied for spinal cord injury (SCI) treatment due to their paracrine action upon damaged tissues. MSCs neuroregenerative role may relate the contents of secretome in anti-inflammatory cytokines and growth-permissive factors. We propose using isolated from adipose tissue—adipose tissue-derived cells (ASCs) as a cell-free based therapy SCI. In vivo studies were conducted two SCI models, Xenopus laevis mice, after complete transection. Our...
Toward the aim of reducing animal testing, innovative in vitro models are required. Here, this study proposes a novel smart polymeric microscaffold to establish an advanced 3D model dopaminergic neurons. These scaffolds fabricated with Ormocomp via Two-Photon Polymerization. The further enhanced by functionalization fluorescent nanodiamonds (FNDs), which can serve as quantum nanosensors for both magnetic and temperature sensing. material biocompatibility is tested using two different cell...
Background and Purpose Spinal cord injury (SCI) is a neurological condition that affects motor sensory functions below the site. The consequences of SCI are devastating for patients, although significant efforts have been done in last years, there no effective therapy. Baclofen has emerged few years as an interesting drug field. Already used clinical setting to control spasticity, baclofen shown important impact on recovery animal models, such lampreys mice. Experimental Approach Key Results...
Exposure to electric fields affects cell membranes impacting their potential and altering cellular excitability, nerve transmission, or muscle contraction. Furthermore, stimulation influences communication, migration, proliferation, differentiation, with therapeutic applications. In vitro platforms for electrical are valuable tools studying these effects advancing medical research. this study, we developed tested a novel multi-channel in stimulator designed The device aims facilitate...
Innovations in spinal cord injury (SCI) models are crucial for developing effective therapies. This study introduces a novel vitro SCI model using cultures of primary mixed cells from rat pups, featuring key cell types. offers distinct advantages terms feasibility, reproducibility, and cost-effectiveness, requiring only basic culture equipment. Following hyperosmotic stress via sorbitol treatment, the recapitulated pathophysiological hallmarks, with 65% reduction viability gradual death over...
Parkinson’s Disease (PD) is characterized by the massive loss of dopaminergic neurons, leading to appearance several motor impairments. Current pharmacological treatments, such as use levodopa, are yet unable cure disease. Therefore, there a need for novel strategies, particularly those that can combine in an integrated manner neuroprotection and neuroregeneration properties. In vitro vivo models have recently revealed secretome mesenchymal stem cells (MSCs) holds promising potential...
Parkinson’s disease (PD) is the most common movement disorder, characterized by progressive loss of dopaminergic neurons from nigrostriatal system. Currently, there no treatment that retards progression or reverses damage prior to time clinical diagnosis. Mesenchymal stem cells (MSCs) are one extensively studied cell sources for regenerative medicine applications, particularly due release soluble factors and vesicles, known as secretome. The main goal this work was address therapeutic...
Parkinson's disease (PD) is the second most common neurodegenerative disorder and characterized by degeneration of dopamine (DA) neurons in substantia nigra pars compacta, leading to a loss DA basal ganglia. The presence aggregates alpha-synuclein (α-synuclein) seen as main contributor pathogenesis progression PD. Evidence suggests that secretome mesenchymal stromal cells (MSC) could be potential cell-free therapy for However, accelerate integration this clinical setting, there still need...
The failure of axons to regenerate after a spinal cord injury (SCI) remains one the greatest challenges in neuroscience. initial mechanical trauma is followed by secondary cascade, creating hostile microenvironment, which not only permissive regeneration but also leads further damage. One most promising approaches for promoting axonal maintain levels cyclic adenosine monophosphate (cAMP), specifically phosphodiesterase-4 (PDE4) inhibitor expressed neural tissues. Therefore, our study, we...
Introduction The inflammatory response after spinal cord injury (SCI) is an important contributor to secondary damage. Infiltrating macrophages can acquire a spectrum of activation states, however, the microenvironment at SCI site favors macrophage polarization into pro-inflammatory phenotype, which one reasons why transplantation has failed. Methods In this study, we investigated therapeutic potential secretome for recovery. We effect in vitro using peripheral and CNS-derived neurons human...
Priming strategies that improve the functionality of MSCs may be required to address issues limiting successful clinical translation MSC therapies. For conditions requiring high trophic support such as brain and spinal cord injuries, priming produce higher levels factors instrumental facilitate current We developed tested a novel molecular paradigm using docosahexaenoic acid (DHA) prime adipose tissue-derived mesenchymal stromal cells (ASCs) enhance secretome neuroregulatory potential.
Neural injuries in cerebral malaria patients are a significant cause of morbidity and mortality. Nevertheless, comprehensive research approach to study this issue is lacking, so herein we propose an vitro system human using cellular approaches. Our first goal was establish identify the molecular alterations brain vasculature cells that resemble blood–brain barrier (BBB) (CM). Through transcriptomic analysis, characterized specific gene expression profiles microvascular endothelial (HBMEC)...
Over 90% of chronic pain (CP) patients receive opioids-based treatments, which led to a public health crisis with lasting impacts on social and economic wellbeing based opioid addiction. Opioids act through activation μ (MOR), δ (DOR), κ (KOR) receptors, are broadly differentially distributed throughout the brain. Chronic consumption leads brain changes such as alterations neurotransmission, dendritic branching, spine density, well an increase in apoptosis. To overcome opioid-related issues,...
Machado–Joseph disease (MJD), also known as spinocerebellar ataxia type 3 (SCA3), is an autosomal dominant neurodegenerative disorder (ND). While most research in NDs has been following a neuron-centric point of view, microglia are now recognized crucial the brain. Previous work revealed alterations that to increased activation state brain CMVMJD135 mice, MJD mouse model replicates motor symptoms and neuropathology human condition. Here, we investigated extent which actively contributing...
In recent years, several studies have demonstrated the crucial role played by sympathetic spleen-innervating neurons in regulating immune cell function involving fighting pathogens or tissue injury. These findings sparked interest across different research fields with a common goal of understanding and manipulating splenic activity to modulate inflammation. However, anatomical identification presents considerable challenge, given multi-compartmentalized location cellular components.This...
Abstract SH‐SY5Y is a human neuroblastoma cell line that can be differentiated into several neuronal phenotypes, depending on culture conditions. For this reason, has been widely used as an in vitro model of neurodegenerative conditions, such Parkinson's disease (PD). However, most studies published to date fetal bovine serum (FBS) medium supplement for differentiation. We report the testing platelet lysate (hPL) support culture. Both standard hPL and fibrinogen‐depleted (FD‐hPL)...