- Antifungal resistance and susceptibility
- Plant Pathogens and Fungal Diseases
- Fungal Infections and Studies
- Mycotoxins in Agriculture and Food
- Gut microbiota and health
- Oral microbiology and periodontitis research
- Dialysis and Renal Disease Management
- Infectious Diseases and Mycology
- Nematode management and characterization studies
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Plant-Microbe Interactions and Immunity
- Cystic Fibrosis Research Advances
- Oral and gingival health research
- Plant Disease Resistance and Genetics
- Clostridium difficile and Clostridium perfringens research
- Bacterial Identification and Susceptibility Testing
- Indoor Air Quality and Microbial Exposure
- Mycorrhizal Fungi and Plant Interactions
- HIV/AIDS oral health manifestations
- Diet and metabolism studies
- Nail Diseases and Treatments
- Plant Pathogenic Bacteria Studies
- Bacterial biofilms and quorum sensing
- Pediatric health and respiratory diseases
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2015-2024
Stemmatters (Portugal)
2024
Universidade do Porto
2012-2023
National Institute of Engineering, Technology and Innovation
2022-2023
Instituto Nacional de Engenharia Biomédica
2022-2023
Centro Hospitalar Tondela-Viseu
2022
Flinders University
2016-2021
Instituto Chico Mendes de Conservação da Biodiversidade
2021
University of South Australia
2020
Flinders Medical Centre
2016-2020
Oral microbiota is one of the most complex and diverse microbial communities in human body. In present study, we aimed to characterize oral fungi biodiversity stability over time a group healthy participants with good health. health fungal were evaluated 40 individuals. A follow-up 10 was carried out 28 weeks 30 after first sampling. rinse collected incubated selective medium at 25ºC 37ºC for 7 days. Fungi identified based on macro- microscopic morphology. API/ID32C used yeast...
The endosphere and the rhizosphere are pertinent milieus with microbial communities that perturb agronomic traits of crop plants through beneficial or detrimental interactions. In this study, we challenged these by adding Streptomyces biocontrol strains to wheat seeds in soils severe Rhizoctonia solani infestation. Wheat were grown a glasshouse standardized system, bacterial fungal microbiomes 233 samples roots (endosphere) monitored for 20 weeks, from seed mature plant stage. results showed...
ABSTRACT The objective of our study was to evaluate parameters influencing the germination Aspergillus conidia. Inoculum concentration and age significantly influenced germination. Different incubation temperatures revealed significant differences among species. internal human milieu provides ideal conditions for development invasive disease by fumigatus but restricts invasion flavus niger .
Vascular calcification (VC) is a frequent condition in chronic kidney disease (CKD) and well-established risk factor for the development of cardiovascular (CVD). Gut dysbiosis may contribute to CVD inflammation CKD patients. Nonetheless, role gut blood microbiomes CKD-associated VC remains unknown. Therefore, this pilot study aimed explore link between patients on peritoneal dialysis (CKD-PD). Our results showed relative changes specific taxa CKD-PD with without VC, namely Coprobacter,...
Abstract Background New fungal species that are morphologically similar to Aspergillus fumigatus were recently described and included in section Fumigati . Misidentification of such species, particularly the human pathogens, lentulus , Neosartorya fischeri hiratsukae pseudofischeri udagawae has been increasingly reported by numerous clinical labs. Nevertheless, A. still accounts for more than 90% all invasive aspergillosis cases. The purpose present study was develop a rapid method molecular...
Abstract Two Pythium ‐infested soils were used to compare the wheat root and rhizosphere soil microbial communities from plants grown in field or greenhouse trials their stability presence of biocontrol agents. Bacteria showed highest diversity at early stages growth both trials, while fungal increased later on, 12 weeks crop cycle. The stable roots samples across types this study. Such was also observed irrespective cultivation system (field greenhouse) addition coatings seeds control...
Factors influencing the occurrence of peritoneal dialysis (PD)-related infections are still far from fully understood. Recent studies described existence specific microbiomes in body sites previously considered microbiome-free, unravelling new microbial pathways human body. In present study, we analyzed peritoneum end-stage kidney disease (ESKD) patients to determine if they harbored a microbiome and it is altered on PD therapy. We conducted cross-sectional study where ESKD with intact...
Amphotericin B and posaconazole susceptibility patterns were determined for the most prevalent Mucorales, following EUCAST (European Committee on Antimicrobial Susceptibility Testing) broth microdilution guidelines. In parallel, Etest was performed evaluated against EUCAST. The overall agreement of MICs gained with 75.1%; therefore, cannot be recommended antifungal testing Mucorales. active drug Mucorales species in vitro, while activities more restricted.
Mucormycosis infections are infrequent yet aggressive and serious fungal infections. Early diagnosis of mucormycosis its discrimination from other is required for targeted treatment more favorable patient outcomes. The majority the molecular assays use 18 S rDNA. In current study, we aimed to explore potential mitochondrial rnl (encoding large-subunit-ribosomal-RNA) gene as a novel marker suitable research diagnostics. Rnl was evaluated for: (1) Mucorales family, (2) species identification...
A new and easy polymerase chain reaction (PCR) multiplex strategy, for the identification of most common fungal species involved in invasive infections (IFI) was developed this work. Two panels with species-specific markers were designed, Candida Panel species, Filamentous Fungi Aspergillus Rhizopusarrhizus. The method allowed correct all targeted pathogens using extracted DNA or by colony PCR, showed no cross-reactivity nontargeted different mixed infections. Sensitivity reached 10 to 1 pg...