A5000189692- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Antibiotic Resistance in Bacteria
- Plant Pathogens and Fungal Diseases
- Nail Diseases and Treatments
- Pneumocystis jirovecii pneumonia detection and treatment
- Bacterial Identification and Susceptibility Testing
- Essential Oils and Antimicrobial Activity
- Mycotoxins in Agriculture and Food
- Bacterial biofilms and quorum sensing
- Genital Health and Disease
- Antimicrobial agents and applications
- Reproductive tract infections research
- Urinary Tract Infections Management
- Enterobacteriaceae and Cronobacter Research
- Infection Control in Healthcare
- Parasitic Infections and Diagnostics
- Pharmaceutical and Antibiotic Environmental Impacts
- Nanocomposite Films for Food Packaging
- Syphilis Diagnosis and Treatment
- Breast Implant and Reconstruction
- Probiotics and Fermented Foods
- Infectious Diseases and Mycology
- Infection Control and Ventilation
- Burn Injury Management and Outcomes
Universidade do Porto
2015-2024
Centre for Health Technology and Services Research
2015-2024
Science Oxford
2023
Hospital de São João
2013-2022
Technologies pour la Santé
2021
Universidade Luterana do Brasil
2019
Inovamais
2017
John Radcliffe Hospital
2012-2017
University of Oxford
2017
Queen's Medical Centre
2016
The composition of the essential oil Thymus pulegioides and its antifungal activity on Candida, Aspergillus dermatophyte fungal strains were studied. Essential from aerial parts plant was obtained by hydrodistillation analysed GC GC-MS. showed high contents carvacrol thymol. MIC minimal lethal concentration used to evaluate against Candida (seven clinical isolates four ATCC type strains), [five isolates, two Colección Española de Cultivos Tipo (CECT) strains] five strains. Antifungal...
We have performed the characterization of adhesion profile, biofilm formation, cell surface hydrophobicity (CSH) and antifungal susceptibility 184 Candida clinical isolates obtained from different human reservoirs. Adhesion was quantified using a flow cytometric assay formation evaluated two methodologies: XTT crystal violet assay. CSH with microbial to hydrocarbons test while planktonic assessed accordingly CLSI protocol for yeast M27-A3 S4. Yeast cells non-albicans species exhibit...
ABSTRACT The increasing recognition and importance of fungal infections, the difficulties encountered in their treatment increase resistance to antifungals have stimulated search for therapeutic alternatives. Essential oils been used empirically. essential Thymus ( vulgaris , T. zygis subspecies mastichina ) often folk medicine. aim present study was evaluate objectively antifungal activity according classical bacteriological methodologies − determination minimal inhibitory concentration...
Five new microsatellite loci were described and characterized for use as molecular markers the identification genetic differentiation of Candida albicans strains. Following typing 72 unrelated clinical isolates, analysis revealed that they all polymorphic, presenting from 5 to 30 alleles 8 46 different genotypes. The discriminatory power obtained by combining information generated three microsatellites used in a multiplex PCR amplification strategy was 0.99, highest ever reported. later test...
Candida parapsilosis, an emergent agent of nosocomial infections, was previously made up a complex three genetically distinct groups (groups I, II, and III). Recently, the C. parapsilosis have been renamed as species: sensu stricto, orthopsilosis, metapsilosis. In Portugal, no data pertaining to distribution antifungal susceptibility these species are yet available. present report, we describe incidence metapsilosis among 175 clinical environmental isolates identified by conventional methods...
Background: Silicone gel breast implants are associated with long-term adverse events, including capsular contracture, reported incidence rates as high 50 percent. However, it is not clear how long the follow-up period should be and whether there any association estrogen or menopausal status. In addition, placement of Baker grade II subjects in majority reports has been data sets controls instead contracture. Methods: A retrospective medical study (1998 to 2004) was performed women (n = 157)...
ABSTRACT In the human fungal pathogen Candida albicans , CUG codon is translated 97% of time as serine and 3% leucine, which potentially originates an array proteins resulting from translation a single gene. Genes encoding cell surface are enriched in codons; thus, mistranslation may influence interactions organism with host. To investigate this, we compared C. strain that misincorporates 28% leucine at CUGs wild-type parental strain. The first displayed increased adherence to inert host...
Biofilms are commonly involved in medical device-related infections. The purpose of this study was to determine the antimicrobial and anti-biofilm activity polyethyleneimine (PEI) PEI-based nanoparticles (nanoPEI) against Staphylococcus aureus, epidermidis, Acinetobacter baumannii Candida albicans (clinical ATCC strains), evaluate their effect upon biofilm formation on polyurethane (PUR)-like catheters. MICs minimal lethal concentrations PEI nanoPEI were determined according CLSI...
ABSTRACT Herein we describe the changes in gene expression profile of Candida parapsilosis associated with acquisition experimentally induced resistance to azole antifungal drugs. Three resistant strains C. were obtained following prolonged vitro exposure a susceptible clinical isolate constant concentrations fluconazole, voriconazole, or posaconazole. We found that after incubation fluconazole became both azoles but not posaconazole, although susceptibility this decreased, whereas strain...
Objectives . The aim of this study was to determine whether irrigation with sodium hypochlorite, chlorhexidine, and ozone gas, alone or in combination, were effective against Enterococcus faecalis Candida albicans ; these are microorganisms frequently isolated from teeth periapical lesions resistant endodontic treatment. Material Methods 220 single root teeth, recently extracted, inoculated formulations tested hypochlorite at 1, 3, 5% chlorhexidine 0.2% 2% gas applied for different periods...
Genetic defects that affect intestinal epithelial barrier function can present with very early-onset inflammatory bowel disease (VEOIBD). Using whole-genome sequencing, a novel hemizygous defect in NOX1 encoding NAPDH oxidase 1 was identified patient ulcerative colitis-like VEOIBD. Exome screening of 1,878 pediatric patients further seven male (IBD) rare mutations. Loss-of-function validated p.N122H and p.T497A, to lesser degree p.Y470H, p.R287Q, p.I67M, p.Q293R as well the previously...
Abstract Background Rapid antimicrobial susceptibility testing (AST) is urgently needed to provide safer treatment counteract resistance. This critical in septic patients, because resistance increases empiric therapy uncertainty and the risk of a poor outcome. We validate novel 2h flow cytometry AST assay directly from positive blood cultures (PBC) by using room temperature stable FASTgramneg FASTgrampos kits (FASTinov® Porto, Portugal) three sites: FASTinov (site-1), Hospital Ramon y Cajal,...
Ibuprofen, a non-steroidal anti-inflammatory drug, exhibited antimicrobial activity against Candida albicans and non-albicans strains. At 10 mg/ml, ibuprofen showed rapid cidal exponential growth phase C. albicans, accompanied by extensive leakage of intracellular K+, permeation to propidium iodide, lysis spheroplasts severe membrane ultrastructural alterations. These results indicate that the killing cells is due direct damage cytoplasmic membrane. 5 inhibited growth; however, it did not...
ABSTRACT The objective of our study was to evaluate parameters influencing the germination Aspergillus conidia. Inoculum concentration and age significantly influenced germination. Different incubation temperatures revealed significant differences among species. internal human milieu provides ideal conditions for development invasive disease by fumigatus but restricts invasion flavus niger .