A5005396831- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Nanocomposite Films for Food Packaging
- DNA and Nucleic Acid Chemistry
- Virus-based gene therapy research
- Periodontal Regeneration and Treatments
- Polymer Surface Interaction Studies
- Shoulder Injury and Treatment
- Advanced Drug Delivery Systems
- Electrospun Nanofibers in Biomedical Applications
- Tendon Structure and Treatment
- Photodynamic Therapy Research Studies
- Porphyrin and Phthalocyanine Chemistry
- Advanced Polymer Synthesis and Characterization
- Surfactants and Colloidal Systems
- Osteoarthritis Treatment and Mechanisms
- Antimicrobial Peptides and Activities
- Hydrogels: synthesis, properties, applications
- Conducting polymers and applications
- Shoulder and Clavicle Injuries
- Immunotherapy and Immune Responses
- biodegradable polymer synthesis and properties
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Hemostasis and retained surgical items
Polytechnique Montréal
2015-2025
École Polytechnique
2003-2017
Université de Montréal
2009
Medical University of Vienna
2007
Polycations having a high buffering capacity in the endosomal pH range, such as polyethylenimine (PEI), are known to be efficient at delivering nucleic acids by overcoming lysosomal sequestration possibly through proton sponge effect, although other mechanisms membrane disruption arising from an interaction between polycation and endosome/lysosome membrane, have been proposed. Chitosan is delivery vehicle for acids, yet its has thought significantly lower than that of PEI, suggesting...
The interaction of chitosan with plasmid DNA was investigated as a function pH, buffer composition, degree deacetylation (DDA), and molecular weight (Mn) chitosan, using isothermal titration microcalorimetry (ITC). Single Set Identical Sites model used to obtain the enthalpy interaction, binding constant, stoichiometry binding. chitosan−DNA shown be coupled proton transfer from revealed by dependence measured heat release on ionization buffer. almost entirely due transfer, because it...
Chitosan (CS) shows in vitro and vivo efficacy for siRNA delivery but with contradictory findings incompletely characterized systems. For understanding which parameters produce effective delivery, a library of precisely chitosans was produced at different degrees deacetylation (DDAs) average molecular weights (Mn). Encapsulation transfection efficiencies were vitro. Formulations selected to examine the influence Mn N:P ratio on nanoparticle uptake, metabolic activity, genotoxicity,...
infection (CDI) is an urgent public health threat with limited preventative options. In this work, we developed a messenger RNA (mRNA)-lipid nanoparticle (LNP) vaccine targeting
Noninvasive visualization of the distribution and persistence mRNA vaccine antigen expression in mammalian systems has implications for development evaluation future vaccines. Here, we genetically fuse E. coli dihydrofolate reductase (eDHFR) to delta furin diproline modified SARS-CoV-2 spike glycoprotein (S2P∆f) image its female mice male non-human primates using [18F]fluoropropyl-trimethoprim ([18F]FP-TMP). Whole body positron emission tomography (PET) imaging revealed transient injection...
Chitosan is a linear cationic biopolymer composed of glucosamine and N-acetyl-glucosamine that only soluble in acidic aqueous solutions precipitates when neutralized. However, it was recently discovered chitosan dissolved containing glycerol phosphate at near neutral pH produced sol−gel transition heated. Understanding this unique thermogelling system requires improved characterization the ionization solubility behaviors chitosan, particular dependencies on temperature, salt, concentration,...
This study was designed to systematically evaluate the influence of pH and serum on transfection process chitosan–DNA complexes, with objective maximizing their efficiency. The hydrodynamic diameter measured by dynamic light scattering (DLS), found increase salt from 243 nm in water 1244 PBS at 7.4 aggregation presence 10% serum. cellular uptake complexes into HEK 293 cells assessed flow cytometry confocal fluorescent imaging lower Based these data, new methodology were tested high levels...
Recently, chitosan dissolved in solutions containing glycerol phosphate (GP) were found to undergo a sol–gel transition when heated and the proposed gelling mechanism was based on increasing hydrophobic interactions with temperature. Subsequently, an investigation of ionization precipitation behavior chitosan, including dependencies temperature, added salt, fraction deacetylated monomers (fD) performed. This latter study revealed important differences temperature dependence pKa versus GP led...
The transfection efficiency (TE) of chitosan–plasmid DNA (pDNA) polyplexes can be critically modulated by the polymer degree deacetylation (DDA) and molecular weight (MW). This study was performed to test hypothesis that TE dependence on chitosan MW DDA is related polyplex stability, hence their intracellular decondensation/unpacking kinetics. Major barriers nonviral gene transfer were studied image-based quantification. Although uptake increased with DDA, it did not appear a...
Chitosan is a family of glucosamine and N-acetyl polysaccharides with poorly understood immune modulating properties. Here, functional U937 macrophage responses were analyzed in response to novel library twenty chitosans controlled degree deacetylation (DDA, 60-98%), molecular weight (1 >100 kDa), acetylation pattern (block vs. random). Specific chitosan preparations (10 or 190 kDa 80% block DDA 3, 5, 10 98% DDA) either induced macrophages release CXCL10 IL-1ra at 5-50 μg/mL, activated the...
Abstract: Chitosan, a natural polymer, is promising system for the therapeutic delivery of both plasmid DNA and synthetic small interfering RNA. Reports attempting to identify optimal parameters chitosan RNA were inconclusive with high molecular weight at amine-to-phosphate (N:P) ratios apparently required efficient transfection. Here we show, first time, that low (LMW-CS) formulations N:P are suitable in vitro LMW-CS nanoparticles positively charged (ζ-potential ~20 mV) an average size...