A5079872793- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Plant Pathogens and Fungal Diseases
- Infectious Diseases and Mycology
- Nail Diseases and Treatments
- Fungal and yeast genetics research
- Bacterial Identification and Susceptibility Testing
- Pneumocystis jirovecii pneumonia detection and treatment
- Mycotoxins in Agriculture and Food
- Yeasts and Rust Fungi Studies
- Plant Disease Resistance and Genetics
- RNA and protein synthesis mechanisms
- Enzyme Production and Characterization
- Fungal Biology and Applications
- Genomics and Chromatin Dynamics
- Phytoplasmas and Hemiptera pathogens
- Microbial infections and disease research
- HIV/AIDS oral health manifestations
- Phytase and its Applications
- Lung Cancer Diagnosis and Treatment
- Plant Pathogenic Bacteria Studies
- Asthma and respiratory diseases
- Tendon Structure and Treatment
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Probiotics and Fermented Foods
Instituto de Salud Carlos III
2016-2025
Centro de Investigación Biomédica en Red
2023-2025
Centro Nacional de Microbiologia
2013-2024
Centro Nacional de Epidemiología
2011-2024
Universidad de La Sabana
2022
Pediatric Oncology Group
2013
Intensive Care Society
2013
Hospital del Niño
2013
Menéndez Pelayo International University
2013
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2007
We have compared the activities of posaconazole and other currently available antifungal agents against a collection 3,378 clinical isolates yeasts filamentous fungi. A total 1,997 Candida spp., 359 yeast species, 697 strains Aspergillus 325 nondermatophyte non-Aspergillus spp. were included. The average geometric means MICs that tested 0.23 microg/ml for amphotericin B, 0.29 flucytosine, 0.97 fluconazole, 0.07 itraconazole, 0.04 voriconazole, 0.15 caspofungin, 0.03 posaconazole....
The glucan synthase complex of the human pathogenic mold Aspergillus fumigatus has been investigated. genes encoding putative catalytic subunit Fks1p and four Rho proteins A. were cloned sequenced. Sequence analysis showed that AfFks1p was a transmembrane protein very similar to other Fksp in yeasts nidulans. Heterologous expression conserved internal hydrophilic domain achieved Escherichia coli. Anti-Fks1p antibodies labeled apex germ tube, as did aniline blue fluorochrome, which specific...
The commercial technique Vitek 2 system for antifungal susceptibility testing of yeast species was evaluated. A collection 154 clinical isolates, including amphotericin B- and azole-resistant organisms, tested. Results were compared with those obtained by the reference procedures both CLSI European Committee on Antimicrobial Susceptibility Testing (EUCAST). Two other techniques approved use, Etest Sensititre YeastOne, included in comparative exercise as well. average essential agreement (EA)...
Background. The benefit of the combination serum galactomannan (GM) assay and polymerase chain reaction (PCR)–based detection Aspergillus DNA for early diagnosis therapy invasive aspergillosis (IA) in high-risk hematological patients remains unclear.
The role of Aspergillus fumigatus 14alpha-sterol demethylase (Cyp51A) in azole drug susceptibility was assessed. Targeted disruption cyp51A azole-susceptible and -resistant strains decreased MICs from 2- to 40-fold. mutants were morphologically indistinguishable the wild-type strain, retaining ability cause pulmonary disease neutropenic mice.
A study was designed to assess the reliability of serial detection Aspergillus sp. DNA diagnose invasive aspergillosis (IA) in patients with febrile neutropenia. Two blood and two serum samples were taken weekly from 83 patients. total 2,244 analyzed by real-time quantitative PCR. Twelve (14.4%) diagnosed IA. Taking consecutive positive results as diagnostic criterion, PCR detected 11 cases, 4 false positives, giving sensitivity, specificity, positive, negative predictive values 91.6%,...
Mucormycetes infections are very difficult to treat and a delay in diagnosis could be fatal for the outcome of patient. A molecular diagnostic technique based on Real Time PCR was developed simultaneous detection Rhizopus oryzae, microsporus genus Mucor spp. both culture clinical samples. The methodology used Molecular beacon species-specific probes with an internal control. This multiplex real-time (MRT-PCR) tested 22 cultured strains 12 samples from patients suffering proven mucormycosis....
ABSTRACT A molecular diagnostic technique based on real-time PCR was developed for the simultaneous detection of three most frequent causative agents fungal opportunistic pneumonia in AIDS patients: Pneumocystis jirovecii , Histoplasma capsulatum and Cryptococcus neoformans / gattii . This tested cultured strains clinical samples from HIV-positive patients. The methodology used involved species-specific beacon probes targeted to internal transcribed spacer regions rDNA. An control also...
The diagnosis of invasive fungal infections (IFIs) is usually based on the isolation fungus in culture and histopathological techniques. However, these methods have many limitations often delaying definitive diagnosis. In recent years, molecular diagnostics emerged as a suitable alternative for IFI When there not clear suspicion involved IFI, panfungal real-time PCR assays been used, allowing amplification any DNA. this approach requires subsequent amplicon sequencing to identify species...
ABSTRACT Galactofuranose-containing molecules have been repeatedly shown to be important antigens among human fungal pathogens, including Aspergillus fumigatus . Immunogenic galactofuran determinants poorly characterized chemically, however. We reported here the characterization of two glycoproteins A. with an N-glycan containing galactofuranose. These proteins are a phospholipase C and phytase. Chemical indicates that it is mixture Hex 5-13 HexNAc 2 oligosaccharides, major molecular species...
Activities of 35 combinations antifungal agents against Scedosporium spp. were analyzed by a checkerboard microdilution design and the summation fractional concentration index. An average indifferent effect was detected apart from azole echinocandins apiospermum. Antagonism absent for all both species.
BackgroundNew techniques, such as those based on multiplex quantitative real-time PCR (MRT-PCR), can improve the detection of invasive candidiasis (IC).
A retrospective analysis of real-time PCR (RT-PCR) results for 151 biopsy samples obtained from 132 patients with proven invasive fungal diseases was performed. PCR-based techniques proved to be fast and sensitive enabled definitive diagnosis in all cases studied, detection a total 28 species.
Although nebulized liposomal amphotericin B (NLAB) is being used in invasive pulmonary aspergillosis (IPA) prophylaxis, no clinical trial has shown its efficacy as a therapeutic strategy. NAIFI the inaugural randomized, controlled designed to examine safety and effectiveness of NLAB (dosage: 25 mg 6 mL, three times per week for weeks) against placebo, auxiliary treatment IPA. Throughout three-year trial, thirteen patients (six NLAB, seven placebo) were included, with 61% onco-hematological...
The antifungal susceptibility results for 32 clinical isolates of Scopulariopsis brevicaulis are presented. Flucytosine and itraconazole were inactive in vitro, MICs amphotericin B, voriconazole, terbinafine all high, with geometric means 13, 25.8, 14.4 microg/ml, respectively.
Interactions in vitro between amphotericin B, itraconazole, voriconazole, and caspofungin against itraconazole-resistant Aspergillus fumigatus clinical strains were determined. Differential results obtained depending on the criteria (MIC or minimal effective concentration) used. Caspofungin voriconazole exhibited most potent interactions, with synergy at least 50% of isolates, average fractional concentration index was 0.38. Antagonism not found for any combination.
The activities of 10 combinations antifungal agents against 25 clinical isolates Scopulariopsis brevicaulis were tested by the checkerboard technique. An average indifferent effect was detected for all combinations. Synergy observed some and combinations, particularly with posaconazole-terbinafine (68% strains), amphotericin B-caspofungin (60%), posaconazole-caspofungin (48%).
The isolation of the pathogenic fungus Histoplasma capsulatum from cultures together with visualization typical intracellular yeast in tissues are gold standard methods for diagnosis histoplasmosis. However, time-consuming, require level 3 containment and experienced personnel, usually call an additional confirmation test. Matrix-Assisted Laser Desorption Ionization Time Flight Mass Spectrometry (MALDI-ToF MS) has been established as a suitable tool microbial identification several clinical...