A5003385861- Antifungal resistance and susceptibility
- Polysaccharides and Plant Cell Walls
- Fungal Infections and Studies
- Fungal and yeast genetics research
- Fungal Biology and Applications
- Mycotoxins in Agriculture and Food
- Enzyme Production and Characterization
- Bacterial biofilms and quorum sensing
- Studies on Chitinases and Chitosanases
- Entomopathogenic Microorganisms in Pest Control
- Plant Pathogens and Fungal Diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Peptidase Inhibition and Analysis
- Neuropeptides and Animal Physiology
- Carbohydrate Chemistry and Synthesis
- Biofuel production and bioconversion
- Galectins and Cancer Biology
- Glycosylation and Glycoproteins Research
- Insect Resistance and Genetics
- Infectious Diseases and Mycology
- Microbial Metabolites in Food Biotechnology
- Probiotics and Fermented Foods
- Plant-Microbe Interactions and Immunity
- Plant and fungal interactions
- Protease and Inhibitor Mechanisms
Institut Pasteur
2012-2021
Aspergillus fumigatus is the most common cause of invasive mold disease in humans. The mechanisms underlying adherence this to host cells and macromolecules have remained elusive. Using mutants with different adhesive properties comparative transcriptomics, we discovered that gene uge3, encoding a fungal epimerase, required for through mediating synthesis galactosaminogalactan. Galactosaminogalactan functions as dominant adhesin A. mediates plastic, fibronectin, epithelial cells. In...
Pulmonary infections due to Aspergillus fumigatus result from the development of a colony tightly associated hyphae in contact with air, either alveoli (invasive aspergillosis) or an existing cavity (aspergilloma). The fungal ball observed vivo resembles aerial obtained agar medium vitro more than mycelial mass liquid shaken conditions that have been classically used date study A. physiology. For this reason, we embarked on analysis characteristics colonies grown static conditions. (i) Under...
The in vivo composition of the mycelial extracellular matrix (ECM) Aspergillus fumigatus during host invasion is reported here for first time. A new galactosaminogalactan and galactomannan were major polysaccharides ECM. ECM varied with aspergillosis pathologies.
The glucan synthase complex of the human pathogenic mold Aspergillus fumigatus has been investigated. genes encoding putative catalytic subunit Fks1p and four Rho proteins A. were cloned sequenced. Sequence analysis showed that AfFks1p was a transmembrane protein very similar to other Fksp in yeasts nidulans. Heterologous expression conserved internal hydrophilic domain achieved Escherichia coli. Anti-Fks1p antibodies labeled apex germ tube, as did aniline blue fluorochrome, which specific...
Abstract The ability of the fungus Aspergillus fumigatus to activate, suppress, or subvert host immune response during life cycle in vivo through dynamic changing cell wall structure and secretion implicates discriminative sensing distinct fungal components. In this study, we have comparatively assessed secreted- membrane-anchored proteins, glycolipids, polysaccharides for induce vaccine-dependent protection transplanted mice Th cytokine production by human-specific CD4+ T clones. results...
Summary Yeast cell walls are critical for maintaining integrity, particularly in the face of challenges such as growth mammalian hosts. The pathogenic fungus Cryptococcus neoformans additionally anchors its polysaccharide capsule to surface via α(1‐3) glucan wall. Cryptococcal cells disrupted their alpha synthase gene were sensitive stresses, including temperature, and showed difficulty dividing. These lacked capsule, although they continued shed material into environment. Electron...
Abstract The opportunistic fungal pathogen Aspergillus fumigatus is increasingly found as a coinfecting agent along with Pseudomonas aeruginosa in cystic fibrosis patients. Amongst the numerous molecules secreted by P. during its growth, phenazines constitute major class. usually four phenazines, pyocyanin (PYO), phenazine-1-carboxamide (PCN), 1-hydroxyphenazine (1-HP) and phenazine-1-carboxylic acid (PCA). These inhibited growth of A. but underlying mechanisms impact these on biology were...
In Aspergillus fumigatus, the conidial surface contains dihydroxynaphthalene (DHN)-melanin. Six-clustered gene products have been identified that mediate sequential catalysis of DHN-melanin biosynthesis. Melanin thus produced is known to be a virulence factor, protecting fungus from host defense mechanisms. present study, individual deletion genes involved in initial three steps melanin biosynthesis resulted an altered with masked rodlet layer, leaky cell wall allowing deposition proteins on...
α-(1,3)-Glucan is a major component of the cell wall Aspergillus fumigatus, an opportunistic human fungal pathogen. There are three genes (AGS1, AGS2 and AGS3) controlling biosynthesis α-(1,3)-glucan in this species. Deletion all AGS resulted triple mutant that was devoid its wall; however, growth germination identical to parental strain vitro. In experimental murine aspergillosis model, less pathogenic than strain. The deletion extensive structural modification conidial wall, especially...
Aspergillus fumigatus is the most common and deadly pulmonary fungal infection worldwide. In lung, fungus usually forms a dense colony of filaments embedded in polymeric extracellular matrix. To identify candidate genes involved this biofilm (BF) growth, we used RNA-Seq to compare transcriptomes BF liquid plankton (PL) growth. Sequencing mapping tens millions sequence reads against A. transcriptome identified 3,728 differentially regulated two conditions. Although many these genes, including...
Mucormycosis is a life-threatening respiratory fungal infection predominantly caused by Rhizopus species. has incompletely understood pathogenesis, particularly how abnormalities in iron metabolism compromise immune responses. Here we show how, as opposed to other filamentous fungi, spp. establish intracellular persistence inside alveolar macrophages (AMs). Mechanistically, lack of swelling conidia results surface retention melanin, which induces phagosome maturation arrest through...
Resistance of Aspergillus fumigatus conidia to desiccation and their capacity reach the alveoli are partly due presence a hydrophobic layer composed protein from hydrophobin family, called RodA, which covers conidial surface. In A. there seven hydrophobins (RodA-RodG) belonging class I III. Most them have never been studied. We constructed single multiple hydrophobin-deletion mutants until generation hydrophobin-free mutant. The phenotype, immunogenicity, virulence were RODA is most...
Summary The first fungal glycosylphosphatidylinositol an‐chored β(1–3)glucanosyltranferase (Gel1p) has been described in Aspergillus fumigatus and its encoding gene GEL1 identified. Glycosylphosphatidylinositol‐anchored glucanosyltransferases play an active role the biosynthesis of cell wall. We characterize here GEL2 , a homologue . Both homologues share common characteristics: (i) are constitutively expressed during over range growth conditions; (ii) Gel2p is also putative GPI‐anchored...
ABSTRACT α(1-3) glucan is a main component of the Aspergillus fumigatus cell wall. In spite its importance, synthesis this amorphous polymer has not been investigated to date. Two genes in A. , AGS1 and AGS2 are highly homologous AGS Schizosaccharomyces pombe which encode putative synthases. The predicted Ags proteins have an estimated molecular mass 270 kDa. were disrupted . Both Δags mutants similar altered hyphal morphologies reduced conidiation levels. Only Δags1 presented reduction...
Galactofuranose (Galf) is a major molecule found in cell wall polysaccharides, secreted glycoproteins, membrane lipophosphoglycans and sphingolipids of Aspergillus fumigatus. The initial step the Galf synthetic pathway re-arrangement UDP-galactopyranose to UDP-Galf through action mutase. A mutant lacking AfUGM1 gene encoding mutase has been constructed. In mutant, though there moderate reduction mycelial growth associated with an increased branching, it remains as pathogenic resistant...
ABSTRACT A triple α1,3 glucan synthase mutant of Aspergillus fumigatus obtained by successive deletions the three genes ( AGS1 , AGS2 and AGS3 ) has a cell wall devoid glucans. The lack glucans affects neither conidial germination nor mycelial vegetative growth is compensated an increase in β1,3 and/or chitin content.