A5060130752- Polysaccharides and Plant Cell Walls
- Fungal Biology and Applications
- Antifungal resistance and susceptibility
- Kawasaki Disease and Coronary Complications
- Microbial Metabolites in Food Biotechnology
- Immune Response and Inflammation
- Enzyme Production and Characterization
- Seaweed-derived Bioactive Compounds
- Vasculitis and related conditions
- Glycosylation and Glycoproteins Research
- Coronary Artery Anomalies
- Streptococcal Infections and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Carbohydrate Chemistry and Synthesis
- Inflammasome and immune disorders
- Fungal Infections and Studies
- Galectins and Cancer Biology
- Polysaccharides Composition and Applications
- Food composition and properties
- Herbal Medicine Research Studies
- Antimicrobial Peptides and Activities
- Pharmacological Effects of Natural Compounds
- Plant pathogens and resistance mechanisms
- Immune Cell Function and Interaction
- Phytochemistry and Bioactivity Studies
Tokyo University of Pharmacy and Life Sciences
2016-2025
Pfizer (United Kingdom)
2021
Seascape Consultants (United Kingdom)
2020
Caterpillar (United States)
2020
Toho University
2019
Toho University Ohashi Medical Center
2019
Nippon Medical School
2019
Kyiv City Clinical Oncology Center
2019
The University of Tokyo
2001-2017
Institute of Science Tokyo
2017
Abstract The cytokine‐inducing activities of fungal polysaccharides were examined in human monocytes culture, with special reference to CD14 and Toll‐like receptors (TLRs). Tumor necrosis factor alpha (TNF‐α) production by was markedly induced a dose‐dependent manner upon stimulation cell walls from Candida albicans mannan Saccharomyces cerevisiae C. , although relatively high concentrations (10 100 μg/ml) stimulants required for activation as compared the lipopolysaccharide (LPS) (1 10...
Dectin 1 is a mammalian cell surface receptor for (1-->3)-beta-d-glucans. Since (1-->3)-beta-d-glucans are commonly present on fungal walls, it has been suggested that dectin important recognizing invasion. In this study we tried to deduce the amino acid residues in responsible beta-glucan recognition. HEK293 cells transfected with mouse cDNA could bind gel-forming (1-->3)-beta-d-glucan, schizophyllan (SPG). The binding of SPG transfectant was inhibited by pretreatment other beta-glucans...
Agaricus blazei is a medically important mushroom widely eaten and prescribed in Japan. Polysaccharide fractions were prepared from cultured A. by repeated extraction with hot water (AgHWE), cold NaOH (AgCA), then (AgHA). By chemical, enzymic, NMR analyses, the primary structures of AgHWE, AgCA, AgHA mainly composed 1,6-beta-glucan. Among these fractions, extracts showed antitumor activity against solid form Sarcoma 180 ICR mice. To demonstrate active component several chemical enzymic...
The ability of grifolan (GRN), a purified fungal (1-->3)-beta-D-glucan, to induce various cytokines from macrophages was examined in vitro. Interleukin-6 (IL-6) activity supernatants the culture macrophage cell line, RAW264.7 dependent on increasing doses GRN. level IL-6 induced with 500 micrograms/ml GRN comparable that lipopolysaccharide (LPS) 10 micrograms/ml. Enhancement mRNA by treatment detected reverse transcriptase-polymerase chain reaction (RT-PCR). effect production also observed...
Experiments have been carried out to characterize the binding of lysozyme (LZM) bacteriol lipopolysaccharide (LPS). The formation LPS·LZM complexes can be readily demonstrated using either physical-chemical separation techniques or a radiolabeled photoaffinity LPS probe. affinity LZM for has estimated ∼ 108 liters/mol. Binding results in loss enzymatic activity by noncompetitive inhibition, as assessed particulate soluble substrates. This interaction with is dictated primarily hydrophobic...
Candida albicans can enter skeletal tissue through a skin wound in an immunocompromised host or by contamination during orthopedic surgery. Such osteomyelitis is accompanied severe pain and bone destruction. It established that nociceptor innervation occurs bone, but the mechanisms of nociceptive modulation fungal inflammation remain unclear. In this study, we show C. stimulates Nav1.8-positive nociceptors via β-glucan receptor Dectin-1 to induce calcitonin gene-related peptide (CGRP). This...
Dectin-1 (gene Clec7a), a receptor for β-glucans, plays important roles in the host defense against fungi and immune homeostasis of intestine. Although this molecule is also suggested to be involved regulation tumorigenesis, role intestinal tumor development remains elucidated. In study, we find that azoxymethane-dextran-sodium-sulfate-induced ApcMin-induced tumorigenesis are suppressed Clec7a-/- mice independently from commensal microbiota. preferentially expressed on myeloid-derived...
Sparassis crispa is an edible mushroom recently cultivable in Japan. Polysaccharide fractions were prepared from the cultured S. by repeated extraction with hot water (SCHWE), cold NaOH (SCCA), and then (SCHA). HWE was further separated 1 volume (SCHWE1v) or 4 volumes (SCHWE4v) of ethanol-precipitable fractions. By chemical, enzymic, NMR analyses, primary structures SCHWE1v, SCCA, SCHA 6-branched 1, 3-β-glucan, having one branch approximately every third mainchain unit. All these showed...
In a previous study, we reported that one of the gel-forming (1-->3)-beta-D-glucans, grifolan (from Grifola frondosa, GRN), stimulated cytokine production from macrophages in vitro. However, several other such as sonifilan (SPG) and SSG, did not induce macrophages. The ultrastructure especially triple- single-helix, does affect cytokine-inducing activity. action on tumor necrosis factor alpha (TNF alpha) release was correlated with molecular weight GRN, since highest fraction Mr > or =...
Abstract The mechanism of host cell recognition Cryptococcus neoformans, an opportunistic fungal pathogen in immunocompromised patients, remains poorly understood. In the present study, we asked whether DNA this yeast activates mouse bone marrow-derived myeloid dendritic cells (BM-DCs). BM-DCs released IL-12p40 and expressed CD40 upon stimulation with cryptococcal DNA, response was abolished by treatment DNase, but not RNase. production expression were attenuated chloroquine, bafilomycin A,...
The innate immune system of humans recognizes the human pathogenic fungus Candida albicans via sugar polymers present in cell wall, such as mannan and beta-glucan. Here, we examined whether nucleic acids from C. activate dendritic cells. DNA induced interleukin-12p40 (IL-12p40) production CD40 expression by murine bone marrow-derived myeloid cells (BM-DCs) a dose-dependent manner. BM-DCs that lacked Toll-like receptor 4 (TLR4), TLR2, dectin-1, which are pattern recognition receptors for...
Abstract Dectin‐1 is known as a sole receptor for β‐glucan, major cell wall component of fungal microorganisms. In the current study, we examined role this molecule in host defense to Cryptococcus neoformans , an opportunistic pathogen AIDS patients. There was no significant difference clinical course and cytokine production between dectin‐1 gene‐deficient control mice. These results indicate that not likely essential development protective responses C. .
Environmental triggers, including those from pathogens, are thought to play an important role in triggering autoimmune diseases, such as vasculitis, genetically susceptible individuals. The mechanism by which activation of the innate immune system contributes vessel-specific autoimmunity vasculitis is not known. Systemic administration Candida albicans water-soluble extract (CAWS) induces aortic root and coronary arteries mice that mimics human Kawasaki disease. We found Dectin-2 signaling...
(1→3)-β-D-Glucans exhibit a variety of biological and immunopharmacological activities, the significance these activities is dependent on structure glucans such as molecular weight, degree branching, conformation. Based generally accepted evidence that conformation clinically used Sonifilan (SPG) triple helix, we prepared alkaline treated SPG (SPG-OH) single helix conformer. In this report, examined (A) antitumor effect solid form tumor in vivo, (B) hematopoietic response cyclophosphamide...
Abstract Polysaccharide β-1,3-D-glucans (β-glucans) are components of the cell wall various fungi and show immunomodulatory activities. β-Glucans have been reported to enhance neutrophil accumulation during pathogenic fungi-induced lung inflammation. Therefore, we examined whether β-glucans themselves possess chemotactic activities for human neutrophils. Among several kinds β-glucans, β-1,6-long glucosyl side-chain-branched β-glucan, isolated from Candida albicans [Candida soluble β-D-glucan...