A5073870257- Immune Response and Inflammation
- Oral microbiology and periodontitis research
- dental development and anomalies
- Peptidase Inhibition and Analysis
- Cancer-related gene regulation
- Bone and Dental Protein Studies
- Wnt/β-catenin signaling in development and cancer
- Bone Metabolism and Diseases
- Periodontal Regeneration and Treatments
- Proteoglycans and glycosaminoglycans research
- Cell Adhesion Molecules Research
- PARP inhibition in cancer therapy
- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- Antimicrobial Peptides and Activities
- Protease and Inhibitor Mechanisms
- Oral and gingival health research
- Toxin Mechanisms and Immunotoxins
- Calcium signaling and nucleotide metabolism
- Bone health and treatments
- Tissue Engineering and Regenerative Medicine
- Fibroblast Growth Factor Research
- Immunotherapy and Immune Responses
- Extracellular vesicles in disease
- Streptococcal Infections and Treatments
Tohoku University
2015-2024
In-Q-Tel
2015
University of Washington
2006
Fundação Dom Cabral
2006
British Society of Periodontology
2004
Kumamoto University
2003
Jagiellonian University
2003
University of Georgia
2003
Niigata University
1996-2002
Okayama University
2002
Abstract The cytokine‐inducing activities of fungal polysaccharides were examined in human monocytes culture, with special reference to CD14 and Toll‐like receptors (TLRs). Tumor necrosis factor alpha (TNF‐α) production by was markedly induced a dose‐dependent manner upon stimulation cell walls from Candida albicans mannan Saccharomyces cerevisiae C. , although relatively high concentrations (10 100 μg/ml) stimulants required for activation as compared the lipopolysaccharide (LPS) (1 10...
In the oral mechanical environment, periodontal ligament cells (PDL cells) contribute to maintaining tissue homeostasis. Recent studies showed that exosomes, small vesicles secreted by a number of cell types, play pivotal role in cell-to-cell communication biological processes. We examined secretion exosomes from PDL stimulated with cyclic stretch and their inflammatory response macrophages using human macrophage line THP-1 primary monocytes/macrophages. prepared supernatants (PDL-sup)...
Abstract Cysteine proteinases (gingipains) elaborated from Porphyromonas gingivalis exhibit enzymatic activities against a broad range of host proteins and are considered key virulence factors in the onset development adult periodontitis defense evasion. In this study, we examined ability arginine-specific gingipains (high molecular mass Arg-specific gingipain (HRGP) 2) lysine-specific (KGP) to cleave monocyte CD14, main receptor for bacterial cell surface components such as LPS. Binding...
Background and Objective Dental follicle cells, putative progenitor cells for cementoblasts, osteoblasts periodontal ligament interplay with Hertwig's epithelial root sheath ( HERS ) during tooth formation, in which is considered to have an inductive role initiating cementogenesis by epithelial–mesenchymal interaction. However, the specific mechanisms controlling cementoblast/osteoblast differentiation of dental are not fully understood. Canonical Wnt signaling has been implicated increased...
Abstract The ultimate goal of periodontal disease treatment is the reorganization functional tissue that can regenerate lost tissue. Regeneration tissues clinically possible by using autogenic transplantation MSCs. However, autologous MSC limited depending on age, systemic and quality, thus precluding their clinical application. Therefore, we evaluated efficacy allogeneic adipose-derived multi-lineage progenitor cells (ADMPC) in a micro-mini pig defect model. ADMPC were isolated from greater...
This report demonstrates that incubation of cytotoxic T cells with NAD causes suppression their ability to proliferate in response stimulator or lyse targets. Effects are evident after for 3 h concentrations as low 1 microM and sustained many hours removal from culture media. Suppression is a result the failure CTL form specific conjugates targets well lower level activation TCR-mediated stimulation, although transmembrane signaling demonstrable. Metabolites such nicotinamide, ADP-ribose,...
ABSTRACT To identify the role in periodontal inflammatory diseases of human gingival fibroblasts (HGF), major constituents tissue, expression CD14, a possible lipopolysaccharide (LPS) receptor, and release soluble CD14 (sCD14) by HGF were examined. Among samples from nine donors tested, more than 50% five expressed but less 20% other four did so, as determined flow cytometric analysis. The on cell surface was correlated with mRNA. skin lung tested no CD18, which indicates that do not possess...
Periodontal diseases are the most prevalent infectious with irreversible loss of tooth supporting apparatus. To reestablish stable health periodontal tissues after healing, concept regenerative surgical procedures has been developed over past three decades, including various grafting materials, guided tissue regeneration (GTR), and use enamel matrix derivatives (EMD) in a clinical setting. More recently, engineering strategies have also applied for regeneration: (1) stem cell therapies; (2)...
Abstract Post-translational modifications are important in regulating the functions of signal proteins. This is well established for intracellular proteins, but little known case extracellular domains cell surface molecules. We recently described a protein, mono-ADP-ribosyltransferase (ADPRT), on cytotoxic T cells and showed that it mediates attachment ADP-ribose to Concomitantly, cytolytic activity proliferation inhibited. Here we report one principal proteins modified by this enzyme...
Receptor-mediated activation of T lymphocytes involves protein phosphorylation by several tyrosine kinases, among those the src-related enzymes p56lck and p59fyn. Accumulating evidence supports notion that these are regulated dephosphorylation, but much is yet to be learned about regulation their activity. Here we demonstrate not p59fyn exists as a complex with 40-kDa protein, which in its ADP-ribosylated form inhibits kinase ADP-ribosylation this mediated an arginine-specific...
Although cementoblasts express Toll-like receptors (TLR)-2 and -4, little is known regarding the possible participation of in inflammatory response. We investigated effects Porphyromonas gingivalis lipopolysaccharide (LPS), tetra- penta-acylated lipid A species (designated PgLPS 1435/1449 1690 , respectively), on gene expression osteoclastogenesis-associated molecules murine cementoblasts. Real-time quantitative RT-PCR analysis revealed that receptor activator NF-κB ligand (RANKL),...
Abstract Aim To evaluate effects of probiotic Lactobacillus reuteri ( L. ) lozenges as an S/RSD adjunct on site‐level changes at molars with deep pockets. Materials and methods 447 molar sites pockets ≥ 5 mm from a previous randomized clinical trial adjunctive for 28 days were analyzed. Multilevel mixed‐effect models (MLM) constructed to analyze outcomes “change in CAL” “pocket closure” (residual PPD < mm) placebo groups 90 180 days. Possible patient‐, tooth‐, predictors analyzed...
Arginine-specific cysteine proteinases (gingipains-R) from periodontopathic Porphyromonas gingivalis cleaved CD14, a bacterial pattern recognition receptor, on human gingival fibroblasts (HGF). Consequently, gingipains-R reduced lipopolysaccharide-induced interleukin-8 production by HGF, indicating that inhibited CD14-dependent HGF activation and are involved in immune evasion the bacterium periodontal tissues.
Cysteine proteinases (gingipains) from Porphyromonas gingivalis are considered key virulence factors of severe periodontitis and host immune evasion. Since expression intercellular adhesion molecule-1 (ICAM-1) on gingival epithelium is indispensable in polymorphonuclear leukocyte (PMN) migration at the site periodontitis, we examined effects gingipains ICAM-1 human oral epithelial cell lines (KB HSC-2) by flow cytometry Western blotting. We found that three purified forms efficiently reduced...
Primary immune responses are initiated by dendritic cells (DC) that inform naive T helper about invading pathogens. DC undergo sequential events leading to irreversible maturation upon bacterial stimulation. To investigate the of during periodontal infection, we studied effects LPS from Porphyromonas gingivalis on DC. generated human peripheral monocytes culture with IL-4 and GM-CSF were incubated P. (Pg LPS) or Escherichia coli (Ec LPS). Flow cytometry real-time quantitative RT-PCR analysis...