A5021086508- Periodontal Regeneration and Treatments
- Oral microbiology and periodontitis research
- Adenosine and Purinergic Signaling
- Proteoglycans and glycosaminoglycans research
- Mesenchymal stem cell research
- Fibroblast Growth Factor Research
- Peptidase Inhibition and Analysis
- dental development and anomalies
- Dental Radiography and Imaging
- Spectroscopy Techniques in Biomedical and Chemical Research
- Immune Response and Inflammation
- Oral Health Pathology and Treatment
- Bone Tissue Engineering Materials
- Extracellular vesicles in disease
- Pancreatic function and diabetes
- Cancer, Hypoxia, and Metabolism
- Dental Health and Care Utilization
- Connective tissue disorders research
- Bone and Dental Protein Studies
- Antimicrobial Peptides and Activities
- Neuroinflammation and Neurodegeneration Mechanisms
- Dental Implant Techniques and Outcomes
- Neonatal Health and Biochemistry
- HIV/AIDS oral health manifestations
- Neurological Complications and Syndromes
Osaka University
2013-2025
Institut Catholique d'Arts et Métiers
2023
In-Q-Tel
2016
Takeda (Japan)
2016
Oklahoma Medical Research Foundation
2003-2008
Abstract Objective Evidence from in vitro, vivo, and clinical studies indicates that adenosine mediates, at least part, the antiinflammatory effects of methotrexate (MTX), although biochemical events involved have not been fully elucidated. This study was undertaken to investigate whether MTX exerts mice lack ecto‐5′‐nucleotidase (ecto‐5′‐NT) (CD73) are unable convert AMP extracellularly, order determine is generated intracellularly transported into extracellular space or dephosphorylation...
Mineralization is the most fundamental process in vertebrates. It predominantly mediated by osteoblasts, which secrete mineral precursors, likely through matrix vesicles (MVs). These vesicular structures are calcium and phosphate rich contain organic material such as acidic proteins. However, it remains largely unknown how intracellular MVs transported secreted. Here, we use scanning electron-assisted dielectric microscopy super-resolution for assessing live osteoblasts mineralizing...
Background: The mechanism of stimulation human gingival epithelial cells (HGEC) by Porphyromonas gingivalis ( Pg ) has not been fully clarified yet. In order to investigate the possible activation HGEC through Toll‐like receptors (TLRs), we analyzed production chemotactic factors and activated nuclear factor‐kappa B (NF‐κB). Methods: mRNA expression TLRs protein TLR2 TLR4 in tissue were assessed using reverse transcription‐polymerase chain reaction (RT‐PCR) assay immunohistochemical...
CD731 is a GPI-anchored cell surface protein with ecto-5'-nucleotidase enzyme activity that plays crucial role in adenosine production. While the roles of receptors (AR) on osteoblasts and osteoclasts have been unveiled to some extent, CD73 CD73-generated bone tissue are largely unknown. To address this issue, we first analyzed phenotype CD73-deficient (cd73(-/-)) mice. The mutant male mice showed osteopenia, significant decreases osteoblastic markers. Levels osteoclastic markers were,...
Abstract After an inflammatory stimulus, lymphocyte migration into draining lymph nodes increases dramatically to facilitate the encounter of naive T cells with Ag-loaded dendritic cells. In this study, we show that CD73 (ecto-5′-nucleotidase) plays important role in regulating process. produces adenosine from AMP and is expressed on high endothelial venules (HEV) subsets lymphocytes. Cd73−/− mice have normal sized lymphoid organs steady state, but ∼1.5-fold larger 2.5-fold increased rates...
Abstract Fibroblast growth factor‐2 (FGF‐2) regulates a variety of functions the periodontal ligament (PDL) cell, which is key player during tissue regeneration following breakdown by disease. In this study, we investigated effects FGF‐2 on cell migration and related signaling pathways MPDL22, mouse PDL clone. activated MPDL22 cells phosphorylation phosphatidylinositol 3‐kinase (PI3K) akt. The P13K inhibitors, Wortmannin LY294002, suppressed both akt activation in suggesting that PI3K/akt...
Abstract Osteoblastic mineralization occurs during the early stages of bone formation. During this mineralization, hydroxyapatite (HA), a major component bone, is synthesized, generating hard tissue. Many mechanisms driving biomineralization remain unclear because traditional biochemical assays used to investigate them are destructive techniques incompatible with viable cells. To determine temporal changes in mineralization-related biomolecules at spots, we performed time-lapse Raman imaging...
Abstract The ultimate goal of periodontal disease treatment is the reorganization functional tissue that can regenerate lost tissue. Regeneration tissues clinically possible by using autogenic transplantation MSCs. However, autologous MSC limited depending on age, systemic and quality, thus precluding their clinical application. Therefore, we evaluated efficacy allogeneic adipose-derived multi-lineage progenitor cells (ADMPC) in a micro-mini pig defect model. ADMPC were isolated from greater...
Periodontal disease, a prevalent oral inflammatory condition, is implicated in exacerbating systemic diseases. However, the molecular mechanisms underlying this association remain unclear. In study, we performed RNA sequencing of gingival tissue samples collected from mouse model periodontal disease at multiple time points to investigate dynamic transcriptomic changes during progression. Our analysis revealed distinct temporal gene expression patterns associated with key and immune response...
Although annexin A1 (ANXA1) is known to mediate inflammatory responses through N-formyl peptide receptor 2 (FPR2), the role of ANXA1-FPR2 signaling pathway in periodontal disease remains unclear. This study investigated contribution this pathophysiology disease. Using a ligature-induced mouse model, we performed histologic analyses examine ANXA1 and FPR2 expression. We observed upregulation within gingiva ligament. In vitro analysis human ligament cells revealed that interleukin 1β...
Abstract Basic fibroblast growth factor (FGF‐2) can enhance biological potentials of periodontal ligament cells and its topical application induces considerable tissue regeneration in vivo. In this study, we examined the effect FGF‐2 on production hyaluronan (HA), an extracellular matrix playing important roles homeostasis inflammatory/wound healing responses, by human (HPDL) cells. An inhibition binding‐protein assay revealed that significantly increased HA HPDL a dose dependent manner....
The new 2018 classification of periodontal diseases is reported to be related tooth loss due disease (TLPD) during supportive therapy (SPT). However, few reports have evaluated this relationship for Asians or analyzed the association and TLPD by distinguishing between active (APT) SPT. In study, we retrospectively applied 607 Japanese periodontitis patients examined annual rates per patient respective periods. were higher in stage IV and/or grade C both APT SPT was not associated with...
ABSTRACT Periodontal tissue supports teeth in the alveolar bone socket via fibrous attachment of periodontal ligament (PDL). The PDL contains fibroblasts and stem/progenitor cells, collectively known as cells (PDLCs), on top osteoblasts cementoblasts surface cementum, respectively. However, characteristics lineage hierarchy each cell type remain poorly defined. This study identified associated protein-1 (Plap-1) a PDL-specific extracellular matrix protein. We generated knock-in mice...
There has been an increasing desire for the development of predictive periodontal regenerative therapy severe periodontitis. In this study, we investigated effect combined use fibroblast growth factor-2 (FGF-2), a drug regeneration approved in Japan, and carbonated apatite (CO3Ap), bioresorbable osteoconductive scaffold, on beagle dog model one-wall defect (severe intraosseous defect) 24 weeks comparison with CO3Ap or vehicle alone.One-wall defects were created (mesiodistal width × depth: 4...
Adenosine deaminase (ADA) can localize to the cell surface through its interaction with CD26. Using CD26-transfected cells, we demonstrate that ADA (ecto-ADA) regulate adenosine receptor engagement by degrading extracellular (Ado) inosine. This ability was dependent upon CD26 expression, extent of saturation ecto-ADA, and kinetics cAMP response. Thus, response markedly decreased when cells were incubated an exogenous source increase ecto-ADA expression. The inhibit demonstrated treatment...
Abstract Background Cellular responses to hypoxia regulate various biological events, including angiogenesis and extracellular matrix metabolism. Collagen is a major component of the in periodontal tissues its coordinated production essential for tissue homeostasis. In this study, we investigated effects on collagen human gingival fibroblasts (HGFs) ligament cells (HPDLs). Methods HGFs HPDLs were cultured under either normoxic (20% O 2 ) or hypoxic (1% conditions. Nuclear expression...
Abstract Osteopontin is a protein found in the bone‐related matrix and plays multiple regulatory roles mineralizing non‐mineralizing tissue. In osteogenic cell‐lines, expression of osteopontin increases with progression differentiation, but both function vary cell type its activation state. this study, we examined by clones established from mouse periodontal ligament, response to inorganic phosphate fibroblast growth factor (FGF)‐2, which can induce tissue regeneration. The involvement...
Iwayama T, Yanagita M, Mori K, Sawada Ozasa Kubota Miki Kojima Y, Takedachi Kitamura Shimabukuro Hashikawa Murakami S. Adiponectin regulates functions of gingival fibroblasts and periodontal ligament cells. J Periodont Res 2012; 47: 563–571. © 2012 John Wiley & Sons A/S Background Objective: is a cytokine constitutively produced by adipocytes exhibits multiple biological targeting various cell types. However, the effects adiponectin on primary cells are still unexplored. Therefore, we...
L-Ascorbic acid 2-phosphate magnesium salt (APM) is an L-ascorbic (AsA) derivative developed to improve AsA stability and display effective biochemical characteristics. This study aimed investigate the effects of APM on functions properties human gingival fibroblasts with respect prevention periodontal disease in comparison those AsA.Human were incubated presence or absence sodium (AsANa). Intracellular was analysed by HPLC. Collagen synthesis measured ELISA real-time RT-PCR. reactive oxygen...
PLAP-1 is an extracellular matrix protein that predominantly expressed in the periodontal ligament within tissue. It was previously revealed negatively regulates bone morphogenetic 2 and transforming growth factor β activity through direct interactions. However, interaction between other factors has not been defined. Here, we positively of fibroblast (FGF-2), a critical tissue homeostasis repair. In this study, isolated mouse embryonic fibroblasts (MEFs) from Plap-1 -/- mice generated our...
Diabetes is often associated with increased prevalence and severity of periodontal disease. We hypothesized that gingival epithelial cells modify disease progression predicted hyperglycemia would activate an inflammatory response in human (HGECs).We tested our hypothesis immortalized HGECs (epi 4 cells) isolated from tissue transfected the simian virus 40 T antigen. The epi were cultured high (25 mM, HG) normal (6 NG) glucose conditions.The showed interleukin-8 (IL-8) protein secretion mRNA...