A5064331422- Bone and Dental Protein Studies
- Oral and Maxillofacial Pathology
- dental development and anomalies
- Bone Metabolism and Diseases
- Bone health and treatments
- Salivary Gland Tumors Diagnosis and Treatment
- Bone Tumor Diagnosis and Treatments
- Tumors and Oncological Cases
- Vascular Malformations and Hemangiomas
- Periodontal Regeneration and Treatments
- Proteoglycans and glycosaminoglycans research
- Cancer Diagnosis and Treatment
- Parathyroid Disorders and Treatments
- Bone health and osteoporosis research
- Enzyme function and inhibition
- Sarcoma Diagnosis and Treatment
- Oral and gingival health research
- Bone and Joint Diseases
- Peptidase Inhibition and Analysis
- Head and Neck Cancer Studies
- Connective tissue disorders research
- Glycosylation and Glycoproteins Research
- Head and Neck Surgical Oncology
- Cleft Lip and Palate Research
- Vascular Tumors and Angiosarcomas
Osaka University
2015-2025
Osaka University Hospital
2022-2023
National Medical Research Center of Dentistry and Maxillofacial Surgery
2007
Max Planck Institute for Biology
1998-2002
Osaka Dental University
1991-2001
Max Planck Society
1999-2000
Kumamoto University
1998
Targeted disruption of core binding factor α1 (Cbfa1) showed that Cbfa1 is an essential transcription in osteoblast differentiation and bone formation. Furthermore, both vitro vivo studies plays important roles matrix production mineralization. However, it remains to be clarified how controls differentiation, formation, remodelling. To understand fully the physiological functions Cbfa1, we generated transgenic mice overexpressed osteoblasts using type I collagen promoter. Unexpectedly,...
Abstract Although osteocytes are the most abundant cells in bone, little is known about their function, and no specific marker protein for has been described. Dentin matrix 1 (DMP1) an acidic phosphoprotein expressed tooth organ bone. Our previous work showed that chicken, which not capable of forming tooth, DMP1 messenger RNA (mRNA) highly bone by Northern blot analysis. To clarify significance expression mRNA its was examined chicken rat. In detected only tissues localized preosteocytes...
Periodontal ligament-associated protein-1 (PLAP-1)/asporin is a recently identified novel member of the small leucine-rich repeat proteoglycan family. PLAP-1/asporin involved in chondrogenesis, and its involvement pathogenesis osteoarthritis has been suggested. We report that also expressed specifically predominantly periodontal ligament (PDL) it negatively regulates mineralization PDL cells. In situ hybridization analysis revealed was not only an erupted tooth but dental follicle, which...
Transcellular Mg2+ transport across epithelia, involving both apical entry and basolateral extrusion, is essential for magnesium homeostasis, but molecules involved in extrusion have not yet been identified. Here, we show that CNNM4 the basolaterally located molecule. strongly expressed intestinal epithelia localizes to their membrane. CNNM4-knockout mice showed hypomagnesemia due malabsorption of magnesium, suggesting its role inner parts body. Imaging analyses revealed can extrude by...
Metabolic reprogramming is a malignant phenotype of cancer. Cancer cells utilize glycolysis to fuel rapid proliferation even in the presence oxygen, and elevated coupled lactate fermentation cancer microenvironment. Although has been recognized as metabolic waste product, it become evident that functions not only an energy source but signaling molecule through receptor G-protein-coupled 81 (GPR81) under physiological conditions. However, pathological role GPR81 remains unclear. Here, we show...
Two features make the tooth an excellent model in study of evolutionary innovations: relative simplicity its structure and fact that major tooth-forming genes have been identified eutherian mammals. To understand nature innovation at molecular level, it is necessary to identify homologs other vertebrates. As a first step toward this goal, amelogenin gene cloned characterized selected species monotremes (platypus echidna), reptiles (caiman), amphibians (African clawed toad). Comparisons...
CD731 is a GPI-anchored cell surface protein with ecto-5'-nucleotidase enzyme activity that plays crucial role in adenosine production. While the roles of receptors (AR) on osteoblasts and osteoclasts have been unveiled to some extent, CD73 CD73-generated bone tissue are largely unknown. To address this issue, we first analyzed phenotype CD73-deficient (cd73(-/-)) mice. The mutant male mice showed osteopenia, significant decreases osteoblastic markers. Levels osteoclastic markers were,...
Runx2 is an essential transcription factor for bone and tooth development whose function in odontoblast differentiation remains to be clarified. To pursue this issue, we examined transgenic mice under the control of Col1a1 promoter (Tg(Col1a1-Runx2) mice). Endogenous protein was detected nuclei preodontoblasts, immature odontoblasts, mesenchymal cells dental sac, osteoblasts, while transgene expression odontoblasts osteoblasts. Odontoblasts Tg(Col1a1-Runx2) lost their columnar shape dentin...
Intercellular adhesion molecule-1 (ICAM-1) is a transmembrane glycoprotein in the immunoglobulin superfamily, which plays an important role cell and signal transduction. Although ICAM-1 believed to play several malignancies, it still uncertain whether or not expression contributes cancer progression. In this study, we performed clinicopathological biological analyses of oral squamous carcinoma (SCC). First, examined tongue SCC immunohistochemically, revealed that was expressed predominantly...
RUNX2 and SP7 are essential transcription factors for osteoblast differentiation at an early stage. Although inhibits a late stage, the function of stage is not fully elucidated. Thus, we pursued in differentiation. induced Sp7 expression Runx2−/− calvarial cells. Adenoviral transfer sh-Sp7 into primary osteoblasts reduced Alpl, Col1a1, Bglap2 mineralization, whereas that mineralization transgenic mice under control 2.3 kb Col1a1 promoter showed osteopenia woven-bone like structure cortical...
ADP-ribosylation factor (ARF)-like 4c (ARL4C) expression, induced by a combination of Wnt/β-catenin and EGF/Ras signaling, has been demonstrated to form epithelial morphogenesis. ARL4C overexpression, due signaling alterations, was involved in tumorigenesis. It also reported that expression correlates with DNA hypomethylation the 3'-untranslated region (UTR) gene during lymphogenesis. The current study conducted investigate functions lung tongue cancers. Immunohistochemical analyses tissue...
ABSTRACT Active cell proliferation and turnover in the growth plate is essential for embryonic postnatal bone growth. We performed a lineage tracing of Wnt/β-catenin signaling responsive cells (Wnt-responsive cells) using Axin2CreERT2;Rosa26ZsGreen mice found novel population that resides outermost layer facing Ranvier's groove (RG; perichondrium adjacent to plate). These Wnt-responsive rapidly expanded contributed formation outer from neonatal growing stage but stopped expanding at young...
The novel, severe acute respiratory syndrome coronavirus (SARS-CoV-2) was firstly reported in late December of 2019 and subsequently caused a global outbreak. It has been shown that SARS-CoV-2 uses ACE2 (Angiotensin Converting Enzyme 2) as cellular receptor for host cell entry through the surface unit SARS-CoV spike glycoprotein. In this brief report, we analyze protein expression localization human salivary gland, propose possible role saliva pathogenesis Coronavirus disease (COVID-19).
A case of sclerosing odontogenic carcinoma (SOC) admixed with a benign fibro-osseous lesion (BFOL) is reported herein. 67-year-old male had paresthesia in the mental region. Computed tomography detected an intragnathic mass that was focally expansile disappearance cortical bone, and contained radiolucency radio-opacity. Under pathological diagnosis as lesion, it surgically removed by curettage. Microscopic analysis showed few parts resected materials dispersed thin cords small nests...
Venous malformations (VMs) are the most common vascular malformations. TEK and PIK3CA causal genes of VMs, may be involved in PI3K/AKT pathway. However, downstream mechanisms underlying or mutations VMs not completely understood. This study aimed to identify a possible association between genetic clinicopathological features. A retrospective clinical, pathological, 114 patients with was performed. TEK, PIK3CA, combined TEK/PIK3CA were identified 49 (43%), 13 (11.4%), 2 (1.75%) patients,...
Abstract Background Squamous cell carcinoma (SCC) of the dorsum tongue is extremely rare, and it clinically resembles various benign lesions. Somatic mutations in TP53 some driver genes were implicated development SCC; however, somatic genetic characteristics dorsal SCC remain unknown. With a detailed analysis gene SCC, we aimed to better understand its biology. Methods Four cases initially occurring on evaluated for clinical histological findings immunohistochemical expression p53 p16. Gene...