A5041805267- Bone health and treatments
- Bone Metabolism and Diseases
- Bone and Dental Protein Studies
- Bone health and osteoporosis research
- Parathyroid Disorders and Treatments
- Dental Radiography and Imaging
- Orthodontics and Dentofacial Orthopedics
- Dental Implant Techniques and Outcomes
- Oral and Maxillofacial Pathology
- Bone Tissue Engineering Materials
- Connective tissue disorders research
- Fibroblast Growth Factor Research
- Protease and Inhibitor Mechanisms
- Temporomandibular Joint Disorders
- dental development and anomalies
- Facial Nerve Paralysis Treatment and Research
- Facial Trauma and Fracture Management
- Bone Tumor Diagnosis and Treatments
- Vitamin K Research Studies
- Periodontal Regeneration and Treatments
- Alkaline Phosphatase Research Studies
- Body Image and Dysmorphia Studies
- Orthopaedic implants and arthroplasty
- Forensic Anthropology and Bioarchaeology Studies
- Heterotopic Ossification and Related Conditions
Universidade Federal de Sergipe
2015-2024
Universidade de Brasília
2018
Niigata University
2005-2017
Hokkaido University
2009-2017
Hospital Estadual Mário Covas
2011-2017
Nagasaki University
2016-2017
Evidence supports that daily and once-weekly administration of teriparatide, human (h)PTH(1–34), enhance bone mass in osteoporotic patients. However, it is uncertain whether different frequencies hPTH(1–34) would induce formation similarly terms quantity quality. To investigate issue, mice were subjected to PTH administration, their bones histologically examined. Frequencies 1 time/2 days, time a day, 2 4 times day. Mice allocated either control or 3 dosing regimens: 80 μg/kg per injection...
This study aimed to evaluate whether the immunolocalization of fibroblast growth factor (FGF) 23 and dentin matrix protein 1 (DMP1) is associated with spatial regularity osteocyte lacunar canalicular system(s) (OLCS). Femora 12-weeks-old male ICR mice were fixed 4% paraformaldehyde, decalcified a 10% EDTA solution then embedded in paraffin. We have devised triple staining procedure that combines silver impregnation, alkaline phosphatase (ALPase) immunohistochemistry tartrate-resistant acid...
To elucidate the histological events that follow administration of eldecalcitol, a second-generation vitamin D analog currently awaiting approval as drug for treatment osteoporosis, we employed ovariectomy (OVX) rat model. OVX rats received vehicle or 30ng/kg and sham-operated animals only. Rats were sacrificed after 12weeks had their femora tibiae removed processed histochemical histomorphometrical analyses. When compared with group, osteoclastic number bone resorption parameters...
Abstract This study aimed to investigate the behavior and ultrastructure of osteoblastic cells after intermittent PTH treatment attempted elucidate role osteoclasts on mediation PTH-driven bone anabolism. After administering intermittently wildtype c-fos−/− mice, immunohistochemical, histomorphometrical, ultrastructural, statistical examinations were performed. Structural kinetic parameters related formation increased in PTH-treated whereas osteoclast-deficient there no significant...
To assess the reliability of CEFBOT, an artificial intelligence (AI)-based cephalometry software, for cephalometric landmark annotation and linear angular measurements according to Arnett's analysis.Thirty lateral radiographs acquired with a Carestream CS 9000 3D unit (Carestream Health Inc., Rochester/NY) were used in this study. The 66 landmarks 10 selected analysis identified on each radiograph by trained human examiner (control) CEFBOT (RadioMemory Ltd., Belo Horizonte, Brazil). For both...
Summary Intermittent administration of human parathyroid hormone (1-34) (hPTH(1-34)) promotes anabolic action in bone by stimulating remodeling, while eldecalcitol, an analog active vitamin D3, suppresses osteoclastic resorption, and forms new minimodeling. We have examined the biological effects combined eldecalcitol hPTH(1-34) on 9-week-old Wistar rats that underwent ovariectomy (OVX) or Sham operation. They were divided into a group, OVX with vehicle (OVX group), 10 µg/kg/day (PTH 20...
Osteogenic disorder shionogi (ODS) rats carry a hereditary defect in ascorbic acid synthesis, mimicking human scurvy when fed with an acid-deficient (aa-def) diet. As aa-def ODS were shown to feature disordered bone formation, we have examined the mineralization this rat model. A fibrous tissue layer surrounding trabeculae of tibial metaphyses was found rats, and showed intense alkaline phosphatase activity proliferating cell nuclear antigen-immunopositivity. Many osteoblasts detached from...
This study aimed to elucidate the ultrastructural role of Gla proteins in bone mineralization by means a warfarin-administration model. Thirty-six 4-week-old male F344 rats received warfarin (warfarin group) or distilled water (control group), and were fixed after 4, 8 12 weeks with an aldehyde solution. Tibiae femora employed for histochemical analyses alkaline phosphatase, osteocalcin tartrate-resistant acid histomorphometry electron microscopy. After weeks, there no marked...
We have histologically examined vascular invasion and calcification of the hypertrophic zone during endochondral ossification in matrix metalloproteinase (MMP)-9 deficient (MMP-9-/-) mice their littermates at 3 days, weeks 6 after birth. Capillaries osteoclasts chondro-osseous junction showed an intense MMP-9 immunopositivity, suggesting that they recognize chemical properties cartilaginous matrices, then release for cartilage degradation. CD31-positive capillaries tartrate-resistant acid...
Sclerostin, an osteocyte-derived molecule, has been reported to serve as a negative regulator of osteoblastic activity well bone remodeling. However, there is no report that verified the regional difference for sclerostin synthesis, and in this study we have investigated immunolocalization by comparing dentin matrix protein (DMP) 1, factor broadly expressed tibial metaphyses cortical bone. In metaphyseal primary trabecules, site modeling, strong DMP1-reactivity was observed osteocytic...
Abstract Osteocytes are organized in functional syncytia collectively referred to as the osteocytic lacunar‐canalicular system (OLCS). The osteocytes interconnected through gap junctions between their cytoplasmic processes, which pass narrow passageways canaliculi. There two possible ways molecules can be transported throughout OLCS: via processes and junctions, pericellular space Transport of minerals small a spatially well‐organized OLCS is vital for bone mineral homeostasis,...
Verifying whether periostin affects the distribution of type I collagen, fibronectin and tenascin C in periodontal ligament (PDL) is important to contribute a more thorough understanding that protein's functions. In this study, we have histologically examined incisor PDL mandibles 20 week-old male wild-type periostin-deficient (periostin-/-) mice, by means fibronectin, C, proliferating cell nuclear antigen, matrix metallo-proteinase (MMP)-1 F4/80-positive monocyte/macrophage immunostaining,...
Abstract Purpose To assess bone microarchitecture in maxillary sites grafted with autogenous or xenogenous grafts as well to demonstrate the usefulness of micro CT dental implant research. Materials and Methods Samples ( n = 12) consisting titanium fixation screws covered by at least 0.5–1 mm human were obtained from 17 materials prepared for scanning conventional histology. Bone histomorphometric parameters evaluated three distinct regions (graft region, transitional native region)....
It has been reported that the Mg-insufficient bone is fragile upon mechanical loading, despite its high mineral density, while vitamin K2 (MK-4: menatetrenone) improved strength of bone. Therefore, we aimed to elucidate ultrastructural properties in rats with dietary Mg insufficiency and without MK-4 supplementation. Morphological examinations including histochemistry, transmission electron microscopy, probe microanalysis (EPMA) X-ray diffraction were conducted on femora tibiae 4-week-old...