A5034195360- Dental Radiography and Imaging
- Mesenchymal stem cell research
- Dental Implant Techniques and Outcomes
- Periodontal Regeneration and Treatments
- Orthodontics and Dentofacial Orthopedics
- Tissue Engineering and Regenerative Medicine
- dental development and anomalies
- Oral and Maxillofacial Pathology
- Endodontics and Root Canal Treatments
- Dental Trauma and Treatments
- Facial Trauma and Fracture Management
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced X-ray and CT Imaging
- Medical Imaging Techniques and Applications
- Electrospun Nanofibers in Biomedical Applications
- Neurogenesis and neuroplasticity mechanisms
- Temporomandibular Joint Disorders
- Bone and Dental Protein Studies
- Nerve injury and regeneration
- Pluripotent Stem Cells Research
- Cardiovascular Function and Risk Factors
- Spine and Intervertebral Disc Pathology
- Virus-based gene therapy research
- Legume Nitrogen Fixing Symbiosis
- Cleft Lip and Palate Research
Hasselt University
2016-2025
Biomedical Research Institute
2001-2022
Belgian Nuclear Research Centre
2021
Erasmus University Rotterdam
2020
Transnational University Limburg
1997-2018
Morpho (United States)
2016-2018
John Wiley & Sons (United States)
2016
Mansoura University
2015
KU Leuven
2008-2015
University of Liège
2014
Cell surface polysaccharides have an established role as virulence factors in human bacterial pathogens. Less documented are the biosynthesis and biological functions of beneficial bacteria. We identified a gene cluster that encodes enzymes regulatory transporter proteins for different steps extracellular (EPS) well-documented probiotic strain Lactobacillus rhamnosus GG. Subsequent mutation welE gene, encoding priming glycosyltransferase within this cluster, comparative phenotypic analyses...
Angiogenesis, the formation of capillaries from pre-existing blood vessels, is a key process in tissue engineering. If supply cannot be established rapidly, there insufficient oxygen and nutrient transport necrosis implanted will occur. Recent studies indicate that human dental pulp contains precursor cells, named stem cells (hDPSC) show self-renewal multilineage differentiation capacity. Since these can easily isolated, cultured cryopreserved, they represent an attractive cell source for...
In the present study, we evaluated differentiation potential of human dental pulp stem cells (hDPSCs) toward Schwann cells, together with their functional capacity regard to myelination and support neurite outgrowth in vitro. Successful cell was confirmed at morphological ultrastructural level by transmission electron microscopy. Furthermore, compared undifferentiated hDPSCs, immunocytochemistry ELISA tests revealed increased glial marker expression neurotrophic factor secretion...
Abstract Aim: To determine the human anatomic variability of nasopalatine canal and its characteristics using an anatomical, histological computed tomography (CT) scan evaluation. Materials Methods: Measurements for were carried out on 163 dry skulls 120 upper jaw spiral CT scans, taken from patients pre‐operative planning purposes implant placement in incisor region. Furthermore, four cadaver specimens imaged a high‐resolution magnetic resonance imaging (HR‐MRI) unit. Afterwards, these...
Cell-based therapies are emerging as an alternative treatment option to promote functional recovery in patients suffering from neurological disorders, which the major cause of death and permanent disability. The present study aimed differentiate human dental pulp stem cells (hDPSCs) toward functionally active neuronal vitro. hDPSCs were subjected a two-step protocol. First, induction was acquired through formation neurospheres, followed by maturation, based on cAMP neurotrophin-3 (NT-3)...
Within the field of dental tissue engineering, establishment adequate vascularization is one most important burdens to overcome. As vascular access within tooth restricted by apical foramen, it major importance implement effective strategies in order recreate viable components teeth and periodontal tissues. However, while current regenerative approaches focus on use stem cells (DSCs), little known about these their ability promote angiogenesis. Therefore, present study aimed elucidate...
The purpose of the study is to present and discuss a workflow regarding computer-assisted surgical planning for bimaxillary surgery intermediate splint fabrication. This describes protocol starting from wax bite registration fabrication necessary splint. procedure proof concept replace not only model but also facebow transfer articulator. Three different modalities were utilized obtain this goal: cone beam computed tomography (CBCT), optical dental scanning, 3-dimensional printing. A...
Failure of remyelination underlies the progressive nature demyelinating diseases such as multiple sclerosis. Macrophages and microglia are crucially involved in formation repair demyelinated lesions. Here we show that myelin uptake temporarily skewed these phagocytes toward a disease-resolving phenotype, while sustained intracellular accumulation induced lesion-promoting phenotype. This phenotypic shift was controlled by stearoyl-CoA desaturase-1 (SCD1), an enzyme responsible for...
Scarce access to primary samples and lack of efficient protocols generate oligodendrocytes (OLs) from human pluripotent stem cells (hPSCs) are hampering our understanding OL biology the development novel therapies. Here, we demonstrate that overexpression transcription factor SOX10 is sufficient surface antigen O4-positive (O4+) myelin basic protein-positive OLs hPSCs in only 22 days, including patients with multiple sclerosis or amyotrophic lateral sclerosis. The SOX10-induced O4+...
To evaluate the characteristic artefact patterns associated with teeth root filled Gutta-percha when scanned four cone-beam CT devices.Whilst using soft tissue simulation, ten human premolars were placed in empty sockets a dry skull. Subsequently, skull was 3D Accuitomo 170(®) , WhiteFox(®) Cranex 3D(®) and Scanora following clinical protocols highest resolution reduction. After proper image registration OnDemand3D(®) software (Cybermed, Seoul, Korea), each slice evaluated by three trained...
Mesenchymal stromal cells (MSCs) are multipotent stem with immunosuppressive and trophic support functions. While MSCs from different sources frequently display a similar appearance in culture, they often show differences their surface marker gene expression profiles. Although bone marrow is considered the "gold standard" tissue to isolate classical (BM-MSC), MSC-like currently also derived more easily accessible extra-embryonic tissues such as umbilical cord. In this study, we defined best...
Despite the spontaneous regenerative capacity of peripheral nervous system, large gap nerve injuries (PNIs) require bridging strategies. The limitations and suboptimal results obtained with autografts or hollow conduits in clinic urge need for alternative treatments. Recently, we have described promising neuroregenerative capacities Schwann cells derived from differentiated human dental pulp stem (d-hDPSCs) vitro. Here, extended vitro assays to show pro-angiogenic effects d-hDPSCs, such as...
Adequate vascularization, a restricting factor for the survival of engineered tissues, is often promoted by addition stem cells or appropriate angiogenic growth factors. In this study, human dental pulp (DPSCs) and from apical papilla (SCAPs) were applied in an vivo model regeneration order to compare their regenerative potential confirm previously demonstrated paracrine properties. 3D-printed hydroxyapatite scaffolds containing DPSCs and/or SCAPs subcutaneously transplanted into...
Tooth autotransplantation (TAT) offers a viable biological approach to tooth replacement in children and adolescents. The aim of this study was evaluate the outcome cone-beam computed tomographic (CBCT)–guided TAT compared conventional protocol assess 3-dimensional (3D) patterns healing after CBCT-guided (secondary aim). This included 100 autotransplanted teeth 88 patients. Each experimental group consisted 50 transplants 44 patients (31 males 19 females). mean (SD) age at time surgery 10.7...
Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system (CNS) characterized by focal inflammatory lesions and prominent demyelination. Even though currently available therapies are effective in treating initial stages disease, they unable to halt or reverse progression into progressive stage. Thus far, no repair-inducing treatments for MS patients. Hence, there an urgent need development new therapeutic strategies either targeting destructive immunological...
Abstract Ameloblasts are the specialized dental epithelial cell type responsible for enamel formation. Following completion of development in humans, ameloblasts lost and biological repair or regeneration is not possible. In past, vitro models to study epithelium ameloblast biology were limited freshly isolated primary cells immortalized lines, both with translational potential. recent years, large strides have been made induced pluripotent stem organoid this essential lineage – enabling...